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XYLT2 (NM_022167) Human Tagged ORF Clone
CAT＃: RG208572
- TrueORF®
XYLT2 (tGFP-tagged) - Human xylosyltransferase II (XYLT2)
ORF Plasmid: DDK
"NM_022167" in other vectors (6)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PXYLT2; SOS; XT-II; XT2; xylT-II |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208572 representing NM_022167
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACGGCGCTGGCCA TCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAGGACGAGGCGGGCGAGAAAGG AAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGTTCCAAGGACACAGACAGTTCAGCAGGGCGA CGGGGCAGCACAGGCAGAAGGCATGGGCGCTGGCGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCA AGGTGGTACGGGCAGTAACCAGCCGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGC CCCAGGCCGCCAGAACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCA CACGGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCCAAGTGCGAGA TCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAGTGCCAGCAGGAGATCGCCAA TGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAGGCTGTGCCCCGGCACTGTCAGCTGACTGGG AAGATGAGCCCCGGCATCCAGTGGGATGAGAGCCAAGCCCAGCAGCCCATGGATGGCCCCCCGGTGCGAA TCGCCTACATGCTGGTGGTTCACGGCCGCGCCATCCGCCAGCTGAAGCGTCTCCTCAAGGCCGTTTATCA CGAGCAGCACTTCTTTTACATCCATGTGGACAAGCGTTCCGACTACCTGCACCGGGAGGTGGTGGAGCTG GCCCAGGGCTATGATAACGTGCGGGTGACGCCCTGGCGCATGGTTACCATCTGGGGCGGGGCCAGCCTCC TGACGATGTACCTGCGGAGCATGCGGGACCTGCTAGAGGTGCCTGGCTGGGCCTGGGACTTCTTCATCAA CCTCAGTGCCACTGACTATCCAACCAGGACCAATGAGGAGCTGGTGGCATTCCTATCCAAGAACCGGGAC AAGAATTTCCTCAAGTCACATGGCCGGGACAACTCCAGGTTCATCAAGAAACAGGGCCTGGACCGGCTCT TCCATGAGTGCGACTCACACATGTGGCGCCTGGGCGAGCGGCAGATCCCAGCAGGCATTGTGGTGGATGG CGGTTCTGACTGGTTCGTGCTGACACGCAGCTTTGTGGAGTATGTGGTGTACACAGATGACCCGCTTGTG GCCCAGCTGCGCCAGTTCTACACATACACACTGCTCCCAGCCGAGTCCTTCTTCCACACGGTGCTGGAGA ACAGCCTGGCCTGTGAGACCCTCGTGGACAACAACCTGCGGGTCACCAACTGGAACCGCAAGCTGGGCTG CAAGTGCCAGTACAAGCACATTGTGGACTGGTGTGGCTGCTCCCCCAACGACTTCAAGCCACAGGACTTC CTCCGGCTGCAGCAAGTCTCCAGACCCACCTTCTTCGCCCGGAAGTTCGAGTCGACTGTGAACCAGGAGG TGCTGGAAATCCTGGACTTCCACCTGTACGGCAGCTACCCCCCCGGCACGCCAGCCCTCAAGGCCTACTG GGAGAACACCTACGACGCGGCTGATGGCCCCAGTGGGCTCAGTGATGTCATGCTCACTGCTTACACAGCC TTCGCCCGCCTCAGCCTGCACCATGCCGCCACTGCTGCACCCCCAATGGGCACCCCACTCTGCAGGTTTG AGCCCAGGGGCTTGCCGTCCAGCGTGCACCTGTATTTCTATGACGACCATTTCCAGGGCTACCTGGTGAC GCAGGCGGTGCAGCCCTCAGCCCAGGGGCCGGCAGAGACGCTTGAGATGTGGCTGATGCCCCAAGGGTCG CTGAAGCTGTTGGGGCGCAGTGACCAGGCCAGCCGGCTCCAGAGTCTGGAGGTTGGCACTGATTGGGACC CCAAAGAGCGTCTTTTCCGGAACTTTGGGGGGTTACTGGGGCCGCTGGACGAGCCTGTGGCCGTGCAGCG CTGGGCCCGGGGCCCCAACCTCACAGCCACAGTGGTCTGGATCGACCCAACCTATGTGGTGGCCACATCT TATGACATCACAGTAGATACGGAGACTGAGGTCACGCAATACAAGCCCCCACTGAGCCGGCCCCTGCGGC CAGGGCCCTGGACTGTTCGACTCCTTCAGTTCTGGGAACCGCTGGGTGAGACCCGCTTCCTTGTGCTGCC CTTGACCTTCAACCGCAAACTACCTCTCAGGAAAGATGATGCCAGCTGGCTGCACGCAGGGCCACCCCAC AACGAGTACATGGAGCAGAGTTTCCAGGGCCTGAGTAGCATCCTGAACCTGCCTCAGCCGGAGCTCGCGG AGGAGGCTGCCCAGCGGCACACACAGCTCACAGGCCCTGCGCTCGAGGCCTGGACAGACAGGGAACTGAG CAGCTTCTGGTCCGTGGCTGGACTGTGTGCCATAGGCCCCTCTCCCTGCCCCTCCCTGGAGCCCTGCAGA CTGACCAGCTGGAGCTCTCTGTCCCCCGACCCCAAATCAGAGCTGGGGCCTGTCAAAGCAGACGGGCGAC TCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208572 representing NM_022167
Red=Cloning site Green=Tags(s) MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEAGEKGRQRKPRPLDPGEGSKDTDSSAGR RGSTGRRHGRWRGRAESPGVPVAKVVRAVTSRQRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPP HGDTGSVEGAPQPTDNGFTPKCEIVGKDALSALARASTKQCQQEIANVVCLHQAGSLMPKAVPRHCQLTG KMSPGIQWDESQAQQPMDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSDYLHREVVEL AQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRD KNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAGIVVDGGSDWFVLTRSFVEYVVYTDDPLV AQLRQFYTYTLLPAESFFHTVLENSLACETLVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDF LRLQQVSRPTFFARKFESTVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDAADGPSGLSDVMLTAYTA FARLSLHHAATAAPPMGTPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMWLMPQGS LKLLGRSDQASRLQSLEVGTDWDPKERLFRNFGGLLGPLDEPVAVQRWARGPNLTATVVWIDPTYVVATS YDITVDTETEVTQYKPPLSRPLRPGPWTVRLLQFWEPLGETRFLVLPLTFNRKLPLRKDDASWLHAGPPH NEYMEQSFQGLSSILNLPQPELAEEAAQRHTQLTGPALEAWTDRELSSFWSVAGLCAIGPSPCPSLEPCR LTSWSSLSPDPKSELGPVKADGRLR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022167 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022167.1, NP_071450.1 |
| RefSeq Size | 3567 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 64132 |
| UniProt ID | Q9H1B5 |
| Domains | Branch |
| Protein Families | Transmembrane |
| Protein Pathways | Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208572 | XYLT2 (Myc-DDK-tagged)-Human xylosyltransferase II (XYLT2) |
CNY 3,990.00 |
|
| RC208572L1 | Lenti ORF clone of Human xylosyltransferase II (XYLT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208572L2 | Lenti ORF clone of Human xylosyltransferase II (XYLT2), mGFP tagged |
CNY 5,890.00 |
|
| RC208572L3 | Lenti ORF clone of Human xylosyltransferase II (XYLT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208572L4 | Lenti ORF clone of Human xylosyltransferase II (XYLT2), mGFP tagged |
CNY 5,890.00 |
|
| SC112785 | XYLT2 (untagged)-Human xylosyltransferase II (XYLT2) |
CNY 6,344.00 |
