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PPM1B (NM_177969) Human Tagged ORF Clone
CAT＃: RG209425
- TrueORF®
PPM1B (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3
ORF Plasmid: DDK
"NM_177969" in other vectors (5)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209425 representing NM_177969
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCATTTTTGGATAAACCCAAAACTGAAAAACATAATGCTCATGGTGCTGGGAATGGTTTACGTT ATGGCCTGAGCAGCATGCAAGGATGGAGAGTGGAAATGGAAGATGCACACACAGCTGTTGTAGGTATTCC TCACGGCTTGGAAGACTGGTCATTTTTTGCAGTTTATGATGGTCATGCTGGATCCCGAGTGGCAAATTAC TGCTCAACACATTTATTAGAACACATCACTACTAACGAAGACTTTAGGGCAGCTGGAAAATCAGGATCTG CTCTTGAGCTTTCAGTGGAAAATGTTAAGAATGGTATCAGAACTGGATTTTTGAAAATTGATGAATACAT GCGTAACTTTTCAGACCTCAGAAACGGGATGGACAGGAGTGGTTCAACTGCAGTGGGAGTTATGATTTCA CCTAAGCATATCTACTTTATCAACTGTGGTGATTCACGTGCTGTTCTGTATAGGAATGGACAAGTCTGCT TTTCTACCCAGGATCACAAACCTTGCAATCCAAGGGAAAAGGAGCGAATCCAAAATGCAGGAGGCAGCGT GATGATACAACGTGTTAATGGTTCATTAGCAGTATCTCGTGCTCTGGGGGACTATGATTACAAGTGTGTT GATGGCAAGGGCCCAACAGAACAACTTGTTTCTCCAGAGCCTGAGGTTTATGAAATTTTAAGAGCAGAAG AGGATGAATTTATCATCTTGGCTTGTGATGGGATCTGGGATGTTATGAGTAATGAGGAGCTCTGTGAATA TGTTAAATCTAGGCTTGAGGTATCTGATGACCTGGAAAATGTGTGCAATTGGGTAGTGGACACTTGTTTA CACAAGGGAAGTCGAGATAACATGAGTATTGTACTAGTTTGCTTTTCAAATGCTCCCAAGGTCTCAGATG AAGCGGTGAAAAAAGATTCAGAGTTGGATAAGCACTTGGAATCACGGGTTGAAGAGATTATGGAGAAGTC TGGCGAGGAAGGAATGCCTGATCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATATCCCAAATTTG CCTCCTGGGGGAGGTCTTGCTGGCAAGCGTAATGTTATTGAAGCTGTTTATAGTAGACTGAATCCACATA GAGAAAGTGATGGGGCCTCCGATGAAGCAGAGGAAAGTGGATCACAGGGAAAATTGGTGGAAGCTCTCAG GCAAATGAGAATTAATCATAGGGGAAACTACCGACAACTTCTGGAGGAGATGCTGACTAGTTACAGGCTA GCTAAAGTAGAGGGAGAAGAAAGCCCTGCTGAACCAGCTGCCACAGCTACTTCTTCGAACAGTGATGCTG GAAACCCAGTGACAATGCAGGAAAGCCATACTGAATCAGAAAGTGGTCTTGCTGAATTAGACAGCTCTAA TGAAGATGCAGGGACAAAGATGAGTGGTGAAAAAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209425 representing NM_177969
Red=Cloning site Green=Tags(s) MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANY CSTHLLEHITTNEDFRAAGKSGSALELSVENVKNGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMIS PKHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCV DGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEYVKSRLEVSDDLENVCNWVVDTCL HKGSRDNMSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNL PPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRL AKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177969 |
| ORF Size | 1437 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177969.2, NP_808908.1 |
| RefSeq Size | 1776 bp |
| RefSeq ORF | 579 bp |
| Locus ID | 5495 |
| UniProt ID | O75688 |
| Protein Families | Druggable Genome, Phosphatase, Stem cell - Pluripotency |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209425 | PPM1B (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3 |
CNY 3,656.00 |
|
| RC209425L3 | Lenti-ORF clone of PPM1B (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3 |
CNY 5,890.00 |
|
| RC209425L4 | Lenti-ORF clone of PPM1B (mGFP-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3 |
CNY 5,890.00 |
|
| SC121298 | PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3 |
CNY 2,400.00 |
|
| SC324040 | PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3 |
CNY 2,400.00 |
