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PAK2 (NM_002577) Human Tagged ORF Clone
CAT＃: RG210165
- TrueORF®
PAK2 (tGFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_002577" in other vectors (5)
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CNY 7,448.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PAK65; PAKgamma |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210165 ORF sequence, codon optimized.
Due to the complexity of NM_002577, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGACAACGGAGAACTGGAAGATAAACCTCCCGCCCCACCCGTTCGGATGTCTTCAACTATCTTCA GCACCGGAGGAAAAGATCCACTCTCCGCCAATCACTCCCTGAAGCCTCTGCCGAGCGTTCCGGAGGAAAA AAAACCTCGCCATAAAATTATTTCAATCTTTTCAGGGACAGAGAAGGGGTCCAAGAAAAAAGAAAAAGAA AGACCCGAGATCAGTCCACCATCTGACTTCGAACACACAATCCATGTTGGATTCGACGCCGTGACGGGAG AGTTTACTGGCATGCCAGAGCAGTGGGCGAGGCTGCTGCAAACTAGCAATATCACAAAACTCGAACAGAA AAAGAACCCACAGGCTGTTCTTGACGTACTGAAGTTTTATGACAGTAACACAGTAAAACAAAAATACCTC AGCTTCACCCCACCCGAAAAGGACGGATTCCCTTCAGGTACCCCCGCTTTGAATGCCAAGGGTACTGAGG CCCCAGCTGTGGTGACCGAGGAAGAAGATGACGATGAGGAAACTGCCCCACCCGTTATCGCTCCGCGGCC TGACCACACAAAATCTATCTACACCCGCTCAGTCATAGATCCCGTCCCAGCTCCCGTCGGTGACTCACAC GTCGATGGCGCCGCTAAGTCCTTGGATAAGCAAAAAAAAAAAACAAAGATGACAGACGAGGAGATCATGG AGAAGCTTCGCACCATAGTTAGTATCGGAGATCCTAAGAAGAAATATACACGCTACGAGAAGATTGGGCA AGGCGCTTCTGGCACTGTCTTCACCGCCACCGACGTCGCTCTGGGACAAGAAGTTGCAATAAAACAAATT AATTTGCAAAAACAACCTAAGAAGGAACTCATAATTAACGAGATCCTGGTGATGAAAGAACTTAAAAACC CAAATATCGTCAACTTCCTCGATAGCTATCTCGTGGGTGATGAGCTTTTTGTCGTAATGGAATATCTGGC AGGGGGCAGTCTGACGGATGTGGTGACCGAAACTTGTATGGATGAAGCCCAGATAGCTGCCGTGTGCCGC GAGTGCTTGCAAGCCCTGGAGTTTCTGCATGCCAACCAGGTCATCCACCGAGACATTAAATCCGACAACG TGCTGCTCGGTATGGAAGGTTCTGTAAAGTTGACTGACTTCGGCTTTTGTGCACAAATAACCCCCGAGCA GTCTAAGCGCTCTACTATGGTTGGAACTCCTTATTGGATGGCACCAGAAGTGGTGACACGAAAGGCCTAT GGTCCGAAGGTCGATATTTGGTCCCTCGGGATCATGGCTATAGAAATGGTAGAAGGCGAGCCACCATATC TCAACGAGAACCCCCTGCGGGCGCTGTATCTGATCGCCACTAATGGTACACCAGAGTTGCAGAATCCTGA AAAATTGTCCCCAATTTTCAGAGACTTTCTGAACCGCTGTCTCGAAATGGATGTGGAGAAGCGCGGTAGT GCAAAGGAACTCTTGCAACATCCTTTTCTGAAACTGGCCAAACCCCTGTCTTCATTGACACCACTTATCA TGGCTGCTAAGGAGGCCATGAAAAGCAATAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210165 representing NM_002577
Red=Cloning site Green=Tags(s) MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKE RPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYL SFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSH VDGAAKSLDKQKKKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQI NLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCR ECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAY GPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGS AKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002577 |
| ORF Size | 1572 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002577.4, NP_002568.2 |
| RefSeq Size | 6139 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 5062 |
| UniProt ID | Q13177 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, ErbB signaling pathway, Focal adhesion, MAPK signaling pathway, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway |
| Gene Summary | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210165 | PAK2 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2). Note: ORF is codon optimized |
CNY 5,848.00 |
|
| RC210165L3 | Lenti-ORF clone of PAK2 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2). Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RC210165L4 | Lenti-ORF clone of PAK2 (mGFP-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2). Note: ORF is codon optimized |
CNY 5,890.00 |
|
| SC315439 | PAK2 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2) |
CNY 5,864.00 |
|
| SC327574 | PAK2 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2) |
CNY 5,864.00 |
