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MAN2B2 (NM_015274) Human Tagged ORF Clone
CAT＃: RG210523
- TrueORF®
MAN2B2 (tGFP-tagged) - Human mannosidase, alpha, class 2B, member 2 (MAN2B2)
ORF Plasmid: DDK
"NM_015274" in other vectors (4)
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CNY 11,120.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210523 representing NM_015274
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCTGTGCTGGCTGCCGCTGCTGGCACCGCTCCTGTTGCTGCGACCGCCAGGGGTCCAGTCCG CCGGCCCCATCCGGGCCTTCGTGGTGCCCCACAGCCACATGGACGTGGGCTGGGTCTACACTGTGCAGGA AAGCATGCGGGCGTACGCCGCCAATGTCTACACCTCAGTGGTGGAAGAGCTGGCCCGCGGCCAGCAGCGC CGGTTCATCGCTGTGGAGCAGGAGTTTTTCCGGCTGTGGTGGGATGGCGTCGCCTCGGACCAGCAGAAAT ACCAGGTCCGCCAGCTCCTGGAGGAAGGACGCCTGGAATTTGTCATCGGAGGCCAGGTCATGCATGACGA GGCTGTGACGCACCTTGATGACCAGATCCTGCAGCTCACAGAAGGACACGGGTTTCTCTATGAAACATTT GGGATCCGGCCACAGTTCTCCTGGCACGTTGACCCGTTTGGCGCCTCTGCCACGACGCCCACCCTATTTG CGCTGGCGGGCTTCAATGCCCACCTCGGCTCCCGGATCGACTACGACCTGAAGGCAGCCATGCAGGAGGC CCGGGGGCTGCAGTTCGTGTGGCGAGGGTCCCCATCCCTCTCAGAGCGGCAGGAAATCTTCACGCACATC ATGGACCAGTACAGCTACTGCACCCCGTCCCACATCCCTTTCTCCAACAGGTCAGGATTTTACTGGAATG GCGTGGCTGTCTTCCCCAAGCCTCCCCCAGATGGGGTGTACCCCAACATGAGTGAGCCTGTCACCCCAGC CAACATCAACCTCTATGCCGAGGCCCTGGTGGCCAACGTGAAGCAGAGGGCCGCCTGGTTCCGGACACCG CACGTCCTCTGGCCCTGGGGATGTGACAAGCAGTTCTTCAATGCCTCGGTGCAGTTTGCCAACATGGACC CGCTGCTGGACCACATCAACAGCCATGCTGCCGAGCTCGGTGTCTCGGTGCAGTATGCCACGCTGGGCGA CTACTTCCGTGCCCTGCACGCTCTCAATGTCACCTGGCGTGTCCGCGACCACCACGACTTCCTGCCCTAT TCCACAGAACCATTCCAGGCCTGGACGGGCTTCTACACGTCCCGCAGCTCACTGAAGGGGCTGGCCCGGC GAGCCAGCGCCTTGTTGTATGCCGGGGAGTCCATGTTCACACGCTACCTGTGGCCGGCCCCCCGTGGGCA TCTGGACCCCACCTGGGCCCTGCAGCAGCTCCAGCAGCTTCGCTGGGCCGTCTCCGAGGTCCAGCACCAT GATGCCATCACTGGGACTGAGTCCCCCAAGGTGAGAGACATGTACGCAACGCACCTGGCCTCGGGGATGC TGGGCGTGCGCAAGCTGATGGCCTCCATCGTCCTAGATGAGCTCCAGCCCCAGGCACCCATGGCGGCCAG CTCCGATGCAGGACCTGCAGGACATTTTGCCTCGGTCTACAACCCGCTGGCCTGGACGGTCACCACCATC GTCACCCTGACTGTTGGTTTCCCTGGAGTCCGCGTCACAGATGAGGCGGGCCACCCAGTGCCCTCGCAGA TCCAGAACTCAACAGAGACCCCATCTGCGTATGACCTGCTTATTCTGACCACAATCCCAGGCCTCAGTTA CCGGCACTACAGCATCAGACCCACTGCAGGGGCCCAAGAGGGCACCCAGGAGCCGGCTGCCACTGTGGCG AGCACCCTTCAATTTGGCCGCAGGCTGAGGAGACGCACCAGCCATGCGGGCAGGTACTTGGTGCCTGTGG CAAACGACTGCTACATTGTGCTGCTCGACCAGGATACCAACCTGATGCACAGCATCTGGGAGAGACAGAG TAACCGAACGGTGCGCGTGACCCAGGAATTCCTGGAGTACCACGTCAACAGGGATGTGAAACAGGGCCCC ATTTCCGATAACTACCTGTTCACACCGGGCAAGGCCGCGGTGCCTGCGTGGGAAGCTGTGGAAATGGAGA TTGTGGCGGGACAGCTTGTGACTGAGATCCGGCAGTACTTCTACAGGAACATGACAGCACAGAATTACAC GTATGCAATCCGCTCCCGGCTCACCCATGTGCCGCAGGGCCATGACGGGGAGCTGCTCTGCCACCGGATA GAGCAGGAGTACCAAGCCGGCCCCCTGGAGCTGAACCGTGAGGCTGTCCTGAGGACCAGCACCAACCTAA ACAGCCAGCAGGTCATCTACTCAGACAACAACGGCTACCAGATGCAGCGGAGGCCCTACGTTTCCTATGT GAACAACAGCATCGCCCGGAATTACTACCCCATGGTTCAGTCGGCCTTCATGGAGGATGGCAAAAGCAGG CTTGTGTTGCTGTCGGAGCGGGCACATGGCATCTCCAGCCAAGGGAATGGGCAGGTGGAGGTCATGCTCC ACCGGCGGCTGTGGAACAACTTCGACTGGGACCTGGGCTACAACCTCACGCTGAACGACACCTCAGTCGT CCACCCAGTGCTCTGGCTTCTGCTGGGATCCTGGTCCCTCACCACTGCCCTGCGCCAGAGGAGCGCACTG GCGCTGCAGCACAGGCCCGTGGTGCTGTTCGGAGACCTCGCTGGGACTGCGCCGAAGCTCCCAGGACCCC AGCAGCAAGAGGCCGTGACGCTGCCCCCGAATCTTCACCTGCAGATCCTGAGCATCCCTGGCTGGCGCTA CAGCTCCAACCACACGGAGCACTCTCAGAATCTCCGGAAAGGCCATCGAGGGGAAGCCCAGGCTGACCTC CGCCGTGTCCTGCTGCGGCTCTACCACCTGTATGAAGTGGGCGAGGACCCAGTCCTGTCTCAGCCAGTAA CAGTGAATCTGGAGGCTGTGCTGCAGGCGCTGGGGTCCGTGGTGGCAGTGGAGGAGCGCTCGCTCACAGG GACCTGGGATTTGAGCATGCTGCACCGCTGGAGCTGGAGGACGGGGCCTGGCCGCCACAGAGGTGACACC ACCTCTCCCTCGAGGCCACCAGGAGGCCCCATCATCACCGTCCACCCAAAGGAAATCCGGACGTTCTTTA TTCACTTTCAACAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210523 representing NM_015274
Red=Cloning site Green=Tags(s) MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQR RFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETF GIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHI MDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPPDGVYPNMSEPVTPANINLYAEALVANVKQRAAWFRTP HVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPY STEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHH DAITGTESPKVRDMYATHLASGMLGVRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTI VTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYSIRPTAGAQEGTQEPAATVA STLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNRDVKQGP ISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRI EQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSR LVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSAL ALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADL RRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDT TSPSRPPGGPIITVHPKEIRTFFIHFQQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015274 |
| ORF Size | 3027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015274.1, NP_056089.1 |
| RefSeq Size | 4242 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 23324 |
| UniProt ID | Q9Y2E5 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Other glycan degradation |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210523 | MAN2B2 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 2 (MAN2B2) |
CNY 7,024.00 |
|
| RC210523L3 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 2 (MAN2B2), Myc-DDK-tagged |
CNY 11,970.00 |
|
| RC210523L4 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 2 (MAN2B2), mGFP tagged |
CNY 11,970.00 |
|
| SC124684 | MAN2B2 (untagged)-Human mannosidase, alpha, class 2B, member 2 (MAN2B2) |
CNY 7,032.00 |
