United States
Germany
Japan
United Kingdom
China
ROBO1 (NM_002941) Human Tagged ORF Clone
CAT＃: RG213799
- TrueORF®
ROBO1 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1
ORF Plasmid: DDK
"NM_002941" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 14,024.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DUTT1; SAX3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213799 representing NM_002941
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATGGAAACATGTTCCTTTTTTGGTCATGATATCACTCCTCAGCTTATCCCCAAATCACCTGTTTC TGGCCCAGCTTATTCCAGACCCTGAAGATGTAGAGAGGGGGAACGACCACGGGACGCCAATCCCCACCTC TGATAACGATGACAATTCGCTGGGCTATACAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACCTCGCATT GTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCC GCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTC ACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCT GATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGG AAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGGAGAGCCTGC AGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATGGAAGAAAGATGGCTCTCCA CTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTG ACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGAC TGTCTTAGAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAA TTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCA AATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGACATGGGTTC ATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTACTCTGACTGTTCAAGAACCT CCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAG CAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCA ACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGA TCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGCATATTTGG AAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCCTGTGAATCAGACTGTAGC CGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAG GATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGAT ATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAG TGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATC CCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTGGCAACCAA ATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG GCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCATTAAAGGACTCAAACCTAATGCAATTTAC CTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAA CACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGT TCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAG TCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATCAGACTGGT TAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCTCAGAAAGGGAGTCAACTA TGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACC CTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAA TTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTG TCTGGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCC TTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGGGGTAAAGA GTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGAGGACCAAGTCAGCCTCGC TCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATC CTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGG GTATCAGAAAAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAA CATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCACAATGAC TGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCACTACCTACAGTCGCCCAG CTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAAC TGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAG GATGGGCGTTTTGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACC TCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAA ACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCGAGCAAAT GACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAACACAGGAGGATCCTACAACAGCT CAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAA ACAGGGTGGCATGAACTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGC GAAGAGTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGA TGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGC AGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCCACAGGAA GAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCACCAGCCCGACAGGAGAC GGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGG ACCCCTGGTCTCAGATATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCC AAGATGCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGG AGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGG ACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGTCGCAGCA GCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGCTGGCCGTCGACATTTTC ATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCA GAAAACCAGACCAGCCAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGAT CTTCCACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAG TACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGG AAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCCAGGTGAT CCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGCAGCAAAACGAGACCTTC CACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAA TAATCCCAGAGATCCCAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAA GCAAATGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATA ATGAAGAATTAGAGGAAACTGAAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213799 representing NM_002941
Red=Cloning site Green=Tags(s) MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRI VEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP DEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSP LDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAE FKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP PHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQR SDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRK DGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI PSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIY LFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQ SQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKT LEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIP FLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWII LMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHND CSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLK DGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRAN DTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNS EEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQE ELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVA KMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAA AAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDD LPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGD PREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQ ANVGRRNIAEMQVLGGYERGEDNNEELEETES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002941 |
| ORF Size | 4926 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002941.2, NP_002932.1 |
| RefSeq Size | 6629 bp |
| RefSeq ORF | 4956 bp |
| Locus ID | 6091 |
| UniProt ID | Q9Y6N7 |
| Domains | ig, IGv, IGc2, IG, FN3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance |
| Gene Summary | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213799 | ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 |
CNY 16,440.00 |
|
| RC213799L3 | Lenti-ORF clone of ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 |
CNY 18,340.00 |
|
| RC213799L4 | Lenti-ORF clone of ROBO1 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 |
CNY 18,340.00 |
|
| SC109739 | ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 |
CNY 11,304.00 |
