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GALNT1 (NM_020474) Human Tagged ORF Clone
CAT＃: RG214166
- TrueORF®
GALNT1 (tGFP-tagged) - Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1)
ORF Plasmid: DDK
"NM_020474" in other vectors (6)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GALNAC-T1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214166 representing NM_020474
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAAATTTGCATACTGCAAGGTGGTCCTAGCCACCTCCTTGATTTGGGTACTCTTGGATATGTTCC TGCTGCTTTACTTCAGTGAATGCAACAAATGTGATGAAAAAAAGGAGAGAGGACTTCCTGCTGGAGATGT TCTAGAGCCAGTACAAAAGCCTCATGAAGGTCCTGGAGAAATGGGGAAACCAGTCGTCATTCCTAAAGAG GATCAAGAAAAGATGAAAGAGATGTTTAAAATCAATCAGTTCAATTTAATGGCAAGTGAGATGATTGCAC TCAACAGATCTTTACCAGATGTTAGGTTAGAAGGGTGTAAAACAAAGGTGTATCCAGATAATCTTCCTAC AACAAGTGTGGTGATTGTTTTCCACAATGAGGCTTGGAGCACACTTCTGCGAACTGTCCATAGTGTCATT AATCGCTCACCAAGACACATGATAGAAGAAATTGTTCTAGTAGATGATGCCAGTGAAAGAGACTTTTTGA AAAGGCCTTTAGAGAGTTATGTGAAAAAACTAAAAGTACCAGTTCATGTAATTCGAATGGAACAACGTTC TGGATTGATCAGAGCTAGATTAAAAGGAGCTGCTGTGTCTAAAGGCCAAGTGATCACCTTCCTGGATGCC CATTGTGAGTGTACAGTGGGATGGCTGGAGCCTCTCTTGGCCAGGATCAAACATGACAGGAGAACAGTGG TGTGTCCCATCATCGATGTGATCAGTGATGATACTTTTGAGTACATGGCAGGCTCTGATATGACCTATGG TGGGTTCAACTGGAAGCTCAATTTTCGCTGGTATCCTGTTCCCCAAAGAGAAATGGACAGAAGGAAAGGT GATCGGACTCTTCCTGTCAGGACACCTACCATGGCAGGAGGCCTTTTTTCAATAGACAGAGATTACTTTC AGGAAATTGGAACATATGATGCTGGAATGGATATTTGGGGAGGAGAAAACCTAGAAATTTCCTTTAGGAT TTGGCAGTGTGGAGGAACTTTGGAAATTGTTACATGCTCACATGTTGGACATGTGTTTCGGAAAGCTACA CCTTACACGTTTCCAGGAGGCACAGGGCAGATTATCAATAAAAATAACAGACGACTTGCAGAAGTGTGGA TGGATGAATTCAAGAATTTCTTCTATATAATTTCTCCAGGTGTTACAAAGGTAGATTATGGAGATATATC GTCAAGAGTTGGTCTAAGACACAAACTACAATGCAAACCTTTTTCCTGGTACCTAGAGAATATATATCCT GATTCTCAAATTCCACGTCACTATTTCTCATTGGGAGAGATACGAAATGTGGAAACGAATCAGTGTCTAG ATAACATGGCTAGAAAAGAGAATGAAAAAGTTGGAATTTTTAATTGCCATGGTATGGGGGGTAATCAGGT TTTCTCTTATACTGCCAACAAAGAAATTAGAACAGATGACCTTTGCTTGGATGTTTCCAAACTTAATGGC CCAGTTACAATGCTCAAATGCCACCACCTAAAAGGCAACCAACTCTGGGAGTATGACCCAGTGAAATTAA CCCTGCAGCATGTGAACAGTAATCAGTGCCTGGATAAAGCCACAGAAGAGGATAGCCAGGTGCCCAGCAT TAGAGACTGCAATGGAAGTCGGTCCCAGCAGTGGCTTCTTCGAAACGTCACCCTGCCAGAAATATTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214166 representing NM_020474
Red=Cloning site Green=Tags(s) MRKFAYCKVVLATSLIWVLLDMFLLLYFSECNKCDEKKERGLPAGDVLEPVQKPHEGPGEMGKPVVIPKE DQEKMKEMFKINQFNLMASEMIALNRSLPDVRLEGCKTKVYPDNLPTTSVVIVFHNEAWSTLLRTVHSVI NRSPRHMIEEIVLVDDASERDFLKRPLESYVKKLKVPVHVIRMEQRSGLIRARLKGAAVSKGQVITFLDA HCECTVGWLEPLLARIKHDRRTVVCPIIDVISDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRRKG DRTLPVRTPTMAGGLFSIDRDYFQEIGTYDAGMDIWGGENLEISFRIWQCGGTLEIVTCSHVGHVFRKAT PYTFPGGTGQIINKNNRRLAEVWMDEFKNFFYIISPGVTKVDYGDISSRVGLRHKLQCKPFSWYLENIYP DSQIPRHYFSLGEIRNVETNQCLDNMARKENEKVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLNG PVTMLKCHHLKGNQLWEYDPVKLTLQHVNSNQCLDKATEEDSQVPSIRDCNGSRSQQWLLRNVTLPEIF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020474 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020474.4 |
| RefSeq Size | 3778 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 2589 |
| UniProt ID | Q10472 |
| Domains | RICIN, Glycos_transf_2 |
| Protein Families | Secreted Protein, Transmembrane |
| Protein Pathways | Metabolic pathways, O-Glycan biosynthesis |
| Gene Summary | This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214166 | GALNT1 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1) |
CNY 4,160.00 |
|
| RC214166L1 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214166L2 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1), mGFP tagged |
CNY 6,460.00 |
|
| RC214166L3 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214166L4 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1), mGFP tagged |
CNY 6,460.00 |
|
| SC113088 | GALNT1 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1) |
CNY 4,168.00 |
