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MGAT5B (NM_144677) Human Tagged ORF Clone
CAT＃: RG214591
- TrueORF®
MGAT5B (tGFP-tagged) - Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1
ORF Plasmid: DDK
"NM_144677" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GnT-IX; GnT-VB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214591 representing NM_144677
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCACCGTCAACCCCGATGGGAAGATAATGGTCAGAAGATGCCTGGTCACCCTGAGACCCTTTCGGC TTTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTTCCTGATGACGTCTCTGGGAGGCCAGTTCTCGGC CCGGCGCCTGGGGGACTCGCCATTCACCATCCGCACAGAAGTGATGGGGGGCCCCGAGTCCCGCGGCGTC CTGCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGAAGCGCATGGACGCACTGGCCAGGCTGGAGAACA GCAGTGAGCTGCACCGGGCCGGCGGCGACCTGCACTTTCCCGCAGACAGGATGCCCCCTGGGGCCGGCCT CATGGAGCGGATCCAGGCTATTGCCCAGAACGTCTCCGACATCGCTGTGAAGGTGGACCAGATCCTGCGC CACAGTCTGCTCCTGCACAGCAAGGTGTCAGAAGGCCGGCGGGACCAGTGTGAGGCACCCAGTGACCCCA AGTTCCCTGACTGCTCAGGGAAGGTGGAGTGGATGCGTGCCCGCTGGACCTCTGACCCCTGCTACGCCTT CTTTGGGGTGGACGGCACCGAGTGCTCCTTCCTCATCTACCTCAGTGAGGTCGAGTGGTTCTGCCCCCCG CTGCCCTGGAGGAACCAGACGGCTGCCCAGAGGGCACCCAAGCCCCTCCCCAAAGTCCAGGCAGTTTTCC GAAGCAACCTGTCCCACCTTCTGGACCTGATGGGCAGCGGGAAGGAGTCCCTGATCTTCATGAAGAAGCG GACCAAGAGGCTCACAGCCCAGTGGGCGCTGGCTGCCCAGCGCCTGGCACAGAAGCTGGGGGCCACCCAG AGGGACCAGAAGCAGATCCTGGTCCACATCGGCTTCCTGACGGAGGAGTCCGGGGACGTGTTCAGCCCTC GGGTCCTGAAGGGCGGGCCCCTAGGGGAGATGGTGCAGTGGGCGGACATTCTGACTGCACTCTATGTCCT GGGCCATGGCCTGCGGGTCACAGTCTCCCTGAAGGAGCTGCAGAGTAACTTAGGGGTACCGCCAGGCCGG GGAAGCTGCCCGCTCACCATGCCCCTGCCCTTCGACCTCATCTACACCGACTACCACGGCCTGCAGCAGA TGAAGCGGCACATGGGACTCTCCTTCAAGAAGTACCGGTGCCGAATCAGGGTCATCGACACCTTCGGGAC GGAACCTGCGTACAACCACGAGGAGTACGCCACGCTGCACGGCTACCGGACCAACTGGGGCTACTGGAAC CTCAACCCCAAGCAGTTCATGACCATGTTTCCTCATACCCCCGACAACTCCTTCATGGGCTTCGTGTCCG AGGAGCTCAACGAGACGGAGAAGCGGCTCATCAAAGGCGGCAAGGCCAGCAACATGGCCGTGGTGTACGG CAAGGAGGCGAGCATCTGGAAGGGGAAGGAGAAGTTCCTGGGCATCCTGAACAAATACATGGAGATCCAT GGCACCGTGTACTACGAGAGCCAGCGGCCCCCCGAGGTGCCAGCCTTTGTGAAGAACCACGGCCTCTTAC CGCAGCCTGAGTTTCAGCAGCTGCTGCGCAAGGCCAAACTCTTCATCGGGTTTGGCTTCCCCTACGAGGG CCCCGCCCCCCTGGAGGCCATCGCCAATGGTTGCATCTTCCTGCAGTCCCGCTTCAGCCCGCCCCACAGC TCCCTCAACCACGAGTTCTTCCGAGGCAAGCCCACCTCCAGAGAGGTGTTCTCCCAGCATCCCTACGCGG AGAACTTCATCGGCAAGCCCCACGTGTGGACAGTCGACTACAACAACTCAGAGGAGTTTGAAGCAGCCAT CAAGGCCATTATGAGAACTCAGGTAGACCCCTACCTACCCTACGAGTACACCTGCGAGGGGATGCTGGAG CGGATCCACGCCTACATCCAGCACCAGGACTTCTGCAGAGCTCCAGACCCTGCCCTACCAGAGGCCCACG CCCCGCAGAGCCCCTTTGTCCTGGCCCCCAATGCCACCCACCTCGAGTGGGCTCGGAACACCAGCTTGGC TCCTGGGGCCTGGCCCCCCGCGCACGCCCTGCGGGCCTGGCTGGCCGTGCCTGGGAGGGCCTGCACCGAC ACCTGCCTGGACCACGGGCTAATCTGTGAGCCCTCCTTCTTCCCCTTCCTGAACAGCCAGGACGCCTTCC TCAAGCTGCAGGTGCCCTGTGACAGCACCGAGTCGGAGATGAACCACCTGTACCCGGCGTTCGCCCAGCC TGGCCAGGAGTGCTACCTGCAGAAGGAGCCTCTGCTCTTCAGCTGCGCCGGCTCCAACACCAAGTACCGC CGGCTCTGCCCCTGCCGCGACTTCCGCAAGGGCCAGGTGGCCTTGTGCCAGGGCTGTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214591 representing NM_144677
Red=Cloning site Green=Tags(s) MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGV LRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILR HSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPP LPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQ RDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQSNLGVPPGR GSCPLTMPLPFDLIYTDYHGLQQMKRHMGLSFKKYRCRIRVIDTFGTEPAYNHEEYATLHGYRTNWGYWN LNPKQFMTMFPHTPDNSFMGFVSEELNETEKRLIKGGKASNMAVVYGKEASIWKGKEKFLGILNKYMEIH GTVYYESQRPPEVPAFVKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCIFLQSRFSPPHS SLNHEFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSEEFEAAIKAIMRTQVDPYLPYEYTCEGMLE RIHAYIQHQDFCRAPDPALPEAHAPQSPFVLAPNATHLEWARNTSLAPGAWPPAHALRAWLAVPGRACTD TCLDHGLICEPSFFPFLNSQDAFLKLQVPCDSTESEMNHLYPAFAQPGQECYLQKEPLLFSCAGSNTKYR RLCPCRDFRKGQVALCQGCL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144677 |
| ORF Size | 2370 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144677.3 |
| RefSeq Size | 4246 bp |
| RefSeq ORF | 2373 bp |
| Locus ID | 146664 |
| UniProt ID | Q3V5L5 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]).[supplied by OMIM, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214591 | MGAT5B (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1 |
CNY 8,360.00 |
|
| RC214591L3 | Lenti-ORF clone of MGAT5B (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1 |
CNY 10,260.00 |
|
| RC214591L4 | Lenti-ORF clone of MGAT5B (mGFP-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1 |
CNY 10,260.00 |
|
| SC306180 | MGAT5B (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1 |
CNY 15,200.00 |
