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Phospholipase C beta 1 (PLCB1) (NM_182734) Human Tagged ORF Clone
CAT＃: RG216150
- TrueORF®
PLCB1 (tGFP-tagged) - Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2
ORF Plasmid: DDK
"NM_182734" in other vectors (4)
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CNY 12,830.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1; PLC-I; PLC154; PLCB1A; PLCB1B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216150 representing NM_182734
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCTCAACCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA AGGGCACCAAATTCGTCAAGTGGGATGATGATTCAACTATTGTTACTCCAATTATTTTGAGGACTGACCC TCAGGGATTTTTCTTTTACTGGACAGATCAAAACAAGGAGACAGAGCTACTGGATCTCAGCCTTGTCAAA GATGCCAGATGTGGGAGACACGCCAAAGCTCCCAAGGACCCCAAATTACGTGAACTTTTGGATGTGGGGA ACATCGGGCGCCTGGAGCAGCGCATGATCACAGTGGTGTATGGGCCTGACCTCGTGAACATCTCCCATTT GAATCTCGTGGCTTTCCAAGAAGAAGTGGCCAAGGAATGGACAAATGAGGTTTTCAGTTTGGCAACAAAC CTGCTGGCCCAAAACATGTCCAGGGATGCATTTCTGGAAAAAGCCTATACTAAACTTAAGCTGCAAGTCA CTCCAGAAGGGCGTATTCCTCTCAAAAACATATATCGCTTGTTTTCAGCAGATCGGAAGCGAGTTGAAAC TGCTTTAGAGGCTTGTAGTCTTCCATCTTCAAGGAATGATTCAATACCTCAAGAAGATTTCACTCCAGAA GTGTACAGAGTTTTCCTCAACAACCTTTGCCCTCGACCTGAAATTGATAACATCTTTTCAGAATTTGGTG CAAAAAGCAAACCATATCTTACCGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGCGAGATCCTCG GCTTAATGAAATACTTTATCCACCTCTAAAACAAGAGCAAGTCCAAGTATTGATTGAGAAGTATGAACCC AACAACAGCCTCGCCAGAAAAGGACAAATATCAGTGGATGGGTTCATGCGCTATCTGAGTGGAGAAGAAA ACGGAGTCGTTTCACCTGAGAAACTGGATTTGAATGAAGACATGTCTCAGCCCCTTTCTCACTATTTCAT TAATTCCTCGCACAACACCTACCTCACAGCTGGCCAACTGGCTGGAAACTCCTCTGTTGAGATGTATCGC CAAGTGCTCCTGTCTGGTTGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGACGGACTGCAGAAGAGGAAC CTGTCATCACCCATGGCTTCACCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATTGCGGA GTGTGCATTTAAGACTTCACCTTTTCCAATTCTCCTTTCGTTTGAGAACCATGTGGATTCCCCAAAGCAG CAAGCCAAGATGGCGGAGTACTGCCGACTGATCTTTGGGGATGCCCTTCTCATGGAGCCCCTGGAAAAAT ATCCACTGGAATCTGGAGTTCCTCTTCCAAGCCCTATGGATTTAATGTATAAAATTTTGGTGAAAAATAA GAAGAAATCACACAAGTCATCAGAAGGAAGCGGCAAAAAGAAGCTCTCAGAACAAGCCTCCAACACCTAC AGTGACTCCTCCAGCATGTTCGAGCCCTCATCCCCAGGAGCCGGAGAAGCTGATACGGAAAGTGACGACG ACGATGATGATGATGACTGTAAAAAATCTTCAATGGATGAGGGGACTGCTGGAAGTGAGGCTATGGCCAC AGAAGAAATGTCTAATCTGGTGAACTATATTCAGCCAGTCAAGTTTGAGTCATTTGAAATTTCAAAAAAA AGAAATAAAAGTTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTTGAACAACTCACCAAGTCTCCAG TGGAATTTGTAGAATATAACAAAATGCAGCTTAGCAGGATATATCCAAAAGGAACACGTGTGGATTCATC CAACTATATGCCTCAGCTCTTCTGGAATGCAGGTTGTCAGATGGTGGCACTTAATTTCCAGACAATGGAC CTGGCTATGCAAATAAATATGGGGATGTATGAATACAACGGGAAGAGTGGCTACAGATTGAAGCCAGAGT TCATGAGGAGGCCTGACAAGCATTTTGATCCATTTACTGAAGGCATCGTAGATGGGATAGTGGCAAACAC TTTGTCTGTTAAGATTATTTCAGGTCAGTTTCTTTCTGATAAGAAAGTTGGGACTTACGTGGAAGTAGAT ATGTTTGGTTTGCCTGTGGATACAAGGAGGAAGGCATTTAAGACCAAAACATCCCAGGGAAATGCTGTGA ATCCTGTCTGGGAAGAAGAACCTATTGTGTTCAAAAAGGTGGTTCTTCCTACTCTGGCCTGTTTGAGAAT AGCAGTTTATGAAGAAGGAGGTAAATTCATTGGCCACCGTATCTTGCCAGTGCAAGCCATTCGGCCAGGC TATCACTATATCTGTCTAAGGAATGAAAGGAACCAGCCTCTGACGCTGCCTGCTGTCTTTGTCTACATAG AAGTGAAAGACTATGTGCCAGACACATATGCAGATGTCATCGAAGCTTTATCAAACCCAATCCGATATGT GAACCTGATGGAACAGAGAGCTAAGCAATTGGCTGCTTTGACACTGGAAGATGAAGAAGAAGTAAAGAAA GAGGCTGATCCTGGAGAAACACCATCAGAGGCTCCAAGTGAAGCGAGAACGACTCCAGCAGAAAATGGGG TGAATCACACTACAACCCTGACACCCAAGCCACCCTCCCAGGCTCTCCACAGCCAGCCAGCTCCAGGTTC TGTAAAGGCACCTGCCAAAACAGAAGATCTTATTCAGAGTGTCTTAACAGAAGTGGAAGCACAGACCATC GAAGAACTAAAGCAACAGAAATCGTTTGTGAAACTTCAAAAGAAACACTACAAAGAAATGAAAGACCTGG TTAAGAGACACCACAAGAAAACCACTGACCTTATCAAAGAACACACTACCAAGTATAATGAAATTCAGAA TGACTACTTGAGAAGGAGAGCCGCTTTGGAAAAGTCCGCCAAAAAGGACAGTAAGAAAAAATCGGAACCC AGCAGCCCTGATCATGGTTCATCAACGATTGAGCAAGACCTCGCTGCCCTGGATGCTGAAATGACCCAAA AGTTAATAGACTTGAAGGACAAACAACAGCAGCAGCTGCTTAATCTTCGGCAAGAACAGTATTATAGTGA AAAATACCAGAAGCGAGAACATATTAAACTGCTTATTCAAAAGTTGACGGATGTCGCAGAAGAGTGTCAG AACAATCAGTTAAAGAAGCTCAAAGAAATCTGTGAGAAAGAAAAGAAAGAATTAAAGAAGAAAATGGATA AAAAGAGGCAGGAGAAGATAACAGAAGCTAAATCCAAAGACAAAAGTCAGATGGAAGAGGAGAAGACAGA GATGATCCGGTTATATATCCAGGAAGTGGTGCAGTATATCAAGAGGCTAGAAGAAGCGCAAAGTAAACGG CAAGAAAAACTCGTAGAGAAACACAAGGAAATACGTCAGCAGATCCTGGATGAAAAGCCCAAGCTGCAGG TGGAGCTGGAGCAAGAATACCAAGACAAATTCAAAAGACTGCCCCTCGAGATTTTGGAATTCGTGCAGGA AGCCATGAAAGGAAAGATCAGTGAAGACAGCAATCACGGTTCTGCCCCTCTCTCCCTGTCCTCAGACCCT GGAAAAGTGAACCACAAGACTCCCTCCAGTGAGGAGCTGGGAGGAGACATCCCAGGAAAAGAATTTGATA CTCCTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216150 representing NM_182734
Red=Cloning site Green=Tags(s) MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK DARCGRHAKAPKDPKLRELLDVGNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPE VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP NNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY SDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKK RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMD LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPG YHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK EADPGETPSEAPSEARTTPAENGVNHTTTLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP SSPDHGSSTIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRLYIQEVVQYIKRLEEAQSKR QEKLVEKHKEIRQQILDEKPKLQVELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPLSLSSDP GKVNHKTPSSEELGGDIPGKEFDTPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182734 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182734.1, NP_877398.1 |
| RefSeq Size | 6823 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 23236 |
| UniProt ID | Q9NQ66 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway |
| Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216150 | PLCB1 (Myc-DDK-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2 |
CNY 8,168.00 |
|
| RC216150L3 | Lenti-ORF clone of PLCB1 (Myc-DDK-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2 |
CNY 13,590.00 |
|
| RC216150L4 | Lenti-ORF clone of PLCB1 (mGFP-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2 |
CNY 13,590.00 |
|
| SC126664 | PLCB1 (untagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2 |
CNY 8,176.00 |
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