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MGAM (NM_004668) Human Tagged ORF Clone
CAT＃: RG216305
- TrueORF®
MGAM (tGFP-tagged) - Human maltase-glucoamylase (alpha-glucosidase) (MGAM). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_004668" in other vectors (2)
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CNY 15,200.00
货期*
3周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MG; MGA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216305 ORF sequence, codon optimized.
Due to the complexity of NM_004668, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGAAAGAAGCTGAAAAAATTTACTACTTTGGAGATTGTGCTCAGTGTTCTTCTGCTTGTGTTGT TTATCATCAGTATTGTTCTAATTGTGCTTTTAGCCAAAGAGTCACTGAAATCAACAGCCCCAGATCCTGG GACAACTGGTACCCCAGATCCTGGGACAACTGGTACCCCAGATCCTGGAACAACTGGTACCACACATGCT AGGACAACGGGTCCCCCAGATCCTGGAACAACTGGTACCACTCCTGTTTCTGCTGAATGTCCAGTGGTAA ATGAATTGGAACGAATTAATTGCATCCCTGACCAGCCGCCAACAAAGGCCACATGTGACCAACGTGGCTG TTGCTGGAATCCCCAGGGAGCTGTAAGTGTTCCCTGGTGCTACTATTCCAAGAATCATAGCTACCATGTA GAGGGCAACCTTGTCAACACAAATGCAGGATTCACAGCCCGGTTGAAAAATCTGCCTTCTTCACCAGTGT TTGGCAGCAACGTCGATAACGTCCTCCTGACCGCTGAATATCAGACATCTAACAGATTTCATTTCAAACT CACCGACCAAACCAATAATCGCTTCGAGGTGCCCCACGAACACGTCCAATCATTTAGCGGCAATGCCGCA GCATCTCTGACTTACCAGGTGGAGATTTCCAGACAGCCTTTTAGTATCAAAGTGACCCGGCGCAGTAATA ACCGAGTTCTCTTCGATTCTTCCATTGGGCCTTTGTTGTTTGCAGATCAGTTCCTCCAACTGTCCACCCG CCTGCCTAGTACTAACGTGTATGGCCTCGGAGAGCACGTGCACCAGCAATATCGCCACGATATGAATTGG AAAACTTGGCCCATCTTCAACCGCGACACCACACCTAACGGAAATGGCACCAATCTGTATGGTGCACAAA CATTCTTCCTCTGTCTGGAAGATGCATCCGGGTTGTCATTTGGCGTATTTCTGATGAATTCTAACGCAAT GGAGGTCGTGCTCCAGCCGGCCCCAGCCATCACATACAGAACAATCGGAGGAATCCTGGATTTCTACGTG TTCCTCGGGAACACACCAGAGCAGGTCGTGCAAGAGTATTTGGAACTCATTGGTAGGCCTGCCCTGCCCT CTTACTGGGCTCTGGGTTTTCATCTTTCACGATACGAATATGGCACCCTGGACAACATGAGGGAGGTCGT CGAACGCAACCGAGCCGCCCAACTGCCGTACGACGTGCAGCACGCTGATATAGATTACATGGATGAACGG CGGGACTTTACATATGACTCTGTGGATTTCAAAGGCTTCCCAGAATTCGTGAACGAACTTCATAATAATG GCCAGAAACTGGTAATCATCGTGGACCCTGCTATATCCAACAACTCTTCCTCAAGTAAACCCTATGGCCC ATATGACAGGGGTTCAGATATGAAGATATGGGTGAATAGTTCAGATGGAGTGACTCCACTCATTGGGGAG GTCTGGCCTGGACAAACTGTGTTTCCTGATTATACCAATCCCAACTGTGCTGTTTGGTGGACAAAGGAAT TTGAGCTTTTTCACAATCAAGTAGAGTTTGATGGAATCTGGATTGATATGAATGAAGTCTCCAACTTTGT TGATGGTTCGGTCTCAGGATGTTCCACAAACAACCTAAATAATCCCCCATTCACTCCCAGAATCCTGGAT GGGTACCTGTTCTGCAAGACTCTCTGTATGGATGCAGTGCAGCACTGGGGCAAGCAGTATGACATTCACA ATCTGTATGGCTACTCCATGGCGGTCGCCACAGCAGAAGCTGCCAAGACTGTGTTCCCTAATAAGAGAAG CTTCATTCTGACCCGTTCTACCTTTGCGGGCTCTGGCAAGTTTGCAGCACATTGGTTAGGAGACAACACT GCCACCTGGGATGACCTGAGATGGTCCATCCCTGGCGTGCTTGAGTTCAACCTTTTTGGCATCCCAATGG TGGGTCCTGACATATGTGGCTTTGCTTTGGACACCCCTGAGGAGCTCTGTAGGCGGTGGATGCAGTTGGG TGCATTTTATCCGTTTTCTAGAAATCACAATGGCCAAGGCTACAAGGACCAGGATCCTGCCTCCTTTGGA GCTGACTCCCTGCTGTTGAATTCCTCCAGGCACTACCTTAACATCCGCTATACTCTATTGCCCTACCTAT ACACCCTCTTCTTCCGTGCTCACAGCCGAGGGGACACGGTGGCCAGGCCCCTTTTGCATGAGTTCTACGA GGACAACAGCACTTGGGATGTGCACCAACAGTTCTTATGGGGGCCCGGCCTCCTCATCACTCCAGTTCTG GATGAAGGTGCAGAGAAAGTGATGGCATATGTGCCTGATGCTGTCTGGTATGACTACGAGACTGGGAGCC AAGTGAGATGGAGGAAGCAAAAAGTCGAGATGGAACTTCCTGGAGACAAAATTGGACTTCACCTTCGAGG AGGCTACATCTTCCCCACACAGCAGCCAAATACAACCACTCTGGCCAGTCGAAAGAACCCTCTTGGTCTT ATCATTGCCCTAGATGAGAACAAAGAAGCAAAAGGAGAACTTTTCTGGGATAATGGGGAAACGAAGGATA CTGTGGCCAATAAAGTGTATCTTTTATGTGAGTTTTCTGTCACTCAAAACCGCTTGGAGGTGAATATTTC ACAATCAACCTACAAGGACCCCAATAATTTAGCATTTAATGAGATTAAAATTCTTGGGACGGAGGAACCT AGCAATGTTACAGTGAAACACAATGGTGTCCCAAGTCAGACTTCTCCTACAGTCACTTATGATTCTAACC TGAAGGTTGCCATTATCACAGATATTGATCTTCTCCTGGGAGAAGCATACACAGTGGAATGGAGCATAAA GATAAGGGATGAAGAAAAAATAGACTGTTACCCTGATGAGAATGGTGCTTCTGCCGAAAACTGCACTGCC CGTGGCTGTATCTGGGAGGCATCCAATTCTTCTGGAGTCCCTTTTTGCTATTTTGTCAACGACCTATACT CTGTCAGTGATGTTCAGTATAATTCCCATGGGGCCACAGCTGACATCTCCTTAAAGTCTTCCGTTTATGC CAATGCCTTCCCCTCCACACCCGTGAACCCCCTTCGCCTGGATGTCACTTACCATAAGAATGAAATGCTG CAGTTCAAGATTTATGATCCCAACAAGAATCGGTATGAAGTTCCAGTCCCTCTGAACATACCCAGCATGC CATCCAGCACCCCTGAGGGTCAACTCTATGATGTGCTCATTAAGAAGAATCCATTTGGGATTGAAATTCG CCGGAAGAGTACAGGCACTATAATTTGGGACTCTCAGCTCCTTGGCTTTACCTTCAGTGACATGTTTATC CGCATCTCCACCCGCCTTCCCTCCAAGTACCTCTATGGCTTTGGGGAAACTGAGCACAGGTCCTATAGGA GAGACTTGGAGTGGCACACTTGGGGGATGTTCTCCCGAGACCAGCCCCCAGGGTACAAGAAGAATTCCTA TGGTGTCCACCCCTACTACATGGGGCTGGAGGAGGACGGCAGTGCCCATGGAGTGCTCCTGCTGAACAGC AATGCCATGGATGTGACGTTCCAGCCCCTGCCTGCCTTGACATACCGCACCACAGGGGGAGTTCTGGACT TTTATGTGTTCTTGGGGCCGACTCCAGAGCTTGTCACCCAGCAGTACACTGAGTTGATTGGCCGGCCTGT GATGGTACCTTACTGGTCTTTGGGGTTCCAGCTGTGTCGCTATGGCTACCAGAATGACTCTGAGATCGCC AGCTTGTATGATGAGATGGTGGCTGCCCAGATCCCTTATGATGTGCAGTACTCAGACATCGACTACATGG AGCGGCAGCTGGACTTCACCCTCAGCCCCAAGTTTGCTGGGTTTCCAGCTCTGATCAATCGCATGAAGGC TGATGGGATGCGGGTCATCCTCATTCTGGATCCAGCCATTTCTGGCAATGAGACACAGCCTTATCCTGCC TTCACTCGGGGCGTGGAGGATGACGTCTTCATCAAATACCCAAATGATGGAGACATTGTCTGGGGAAAGG TCTGGCCTGATTTTCCTGATGTTGTTGTGAATGGGTCTCTAGACTGGGACAGCCAAGTGGAGCTATATCG AGCTTATGTGGCCTTCCCAGACTTTTTCCGTAATTCAACTGCCAAGTGGTGGAAGAGGGAAATAGAAGAA CTATACAACAATCCACAGAATCCAGAGAGGAGCTTGAAGTTTGATGGCATGTGGATTGATATGAATGAAC CATCAAGCTTCGTGAATGGGGCAGTTTCTCCAGGCTGCAGGGACGCCTCTCTGAACCACCCTCCCTACAT GCCACATTTGGAGTCCAGGGACAGGGGCCTGAGCAGCAAGACCCTTTGTATGGAGAGTCAGCAGATCCTC CCAGACGGCTCCCTGGTGCAGCACTACAACGTGCACAACCTGTATGGGTGGTCCCAGACCAGACCCACAT ACGAAGCCGTGCAGGAGGTGACGGGACAGCGAGGGGTCGTCATCACCCGCTCCACATTTCCCTCTTCTGG CCGCTGGGCAGGACATTGGCTGGGAGACAACACGGCCGCATGGGATCAGCTGAAGAAGTCTATCATTGGC ATGATGGAGTTCAGCCTCTTCGGCATATCCTATACGGGAGCAGATATCTGTGGGTTCTTTCAAGATGCTG AATATGAGATGTGTGTTCGCTGGATGCAGCTGGGGGCCTTTTACCCCTTCTCAAGAAACCACAACACCAT TGGGACCAGGAGACAAGACCCTGTGTCCTGGGATGTTGCTTTTGTGAATATTTCCAGAACTGTCCTGCAG ACCAGATACACCCTGTTGCCATATCTGTATACCTTGATGCATAAGGCCCACACGGAGGGCGTCACTGTTG TGCGGCCTCTGCTCCATGAGTTTGTGTCAGACCAGGTGACATGGGACATAGACAGTCAGTTCCTGCTGGG CCCAGCCTTCCTGGTCAGCCCTGTCCTGGAGCGTAATGCCAGAAATGTCACTGCATATTTCCCTAGAGCC CGCTGGTATGATTACTACACGGGTGTGGATATTAATGCAAGAGGAGAGTGGAAGACCTTGCCAGCCCCTC TTGACCACATTAATCTTCATGTCCGTGGGGGCTACATCCTGCCCTGGCAAGAGCCTGCACTGAACACCCA CTTAAGCCGCCAGAAATTCATGGGCTTCAAAATTGCCTTGGATGATGAAGGAACTGCTGGGGGCTGGCTC TTCTGGGATGATGGGCAAAGCATTGATACCTATGGGAAAGGACTCTATTACTTGGCCAGCTTTTCTGCCA GCCAGAATACGATGCAAAGCCATATAATTTTCAACAATTACATCACTGGTACAAATCCTTTGAAACTGGG CTACATTGAAATCTGGGGAGTGGGCAGTGTCCCCGTTACCAGTGTCAGCATCTCTGTGAGTGGCATGGTC ATAACACCCTCCTTCAACAATGACCCCACGACACAGGTATTAAGCATCGATGTGACTGACAGAAACATCA GCCTACATAATTTTACTTCATTGACGTGGATAAGCACTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216305 representing NM_004668
Red=Cloning site Green=Tags(s) MARKKLKKFTTLEIVLSVLLLVLFIISIVLIVLLAKESLKSTAPDPGTTGTPDPGTTGTPDPGTTGTTHA RTTGPPDPGTTGTTPVSAECPVVNELERINCIPDQPPTKATCDQRGCCWNPQGAVSVPWCYYSKNHSYHV EGNLVNTNAGFTARLKNLPSSPVFGSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAA ASLTYQVEISRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMNW KTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYV FLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDER RDFTYDSVDFKGFPEFVNELHNNGQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGE VWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNNLNNPPFTPRILD GYLFCKTLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNT ATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFG ADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVL DEGAEKVMAYVPDAVWYDYETGSQVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGL IIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEP SNVTVKHNGVPSQTSPTVTYDSNLKVAIITDIDLLLGEAYTVEWSIKIRDEEKIDCYPDENGASAENCTA RGCIWEASNSSGVPFCYFVNDLYSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEML QFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKNPFGIEIRRKSTGTIIWDSQLLGFTFSDMFI RISTRLPSKYLYGFGETEHRSYRRDLEWHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNS NAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIA SLYDEMVAAQIPYDVQYSDIDYMERQLDFTLSPKFAGFPALINRMKADGMRVILILDPAISGNETQPYPA FTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEE LYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCRDASLNHPPYMPHLESRDRGLSSKTLCMESQQIL PDGSLVQHYNVHNLYGWSQTRPTYEAVQEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIG MMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQ TRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRA RWYDYYTGVDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRQKFMGFKIALDDEGTAGGWL FWDDGQSIDTYGKGLYYLASFSASQNTMQSHIIFNNYITGTNPLKLGYIEIWGVGSVPVTSVSISVSGMV ITPSFNNDPTTQVLSIDVTDRNISLHNFTSLTWISTL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004668 |
| ORF Size | 5571 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004668.2, NP_004659.2 |
| RefSeq Size | 6513 bp |
| RefSeq ORF | 5574 bp |
| Locus ID | 8972 |
| UniProt ID | O43451 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Galactose metabolism, Metabolic pathways, Starch and sucrose metabolism |
| Gene Summary | This gene encodes maltase-glucoamylase, which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase, but the proteins are only 59% homologous. Both are members of glycosyl hydrolase family 31, which has a variety of substrate specificities. [provided by RefSeq, Jul 2008] |
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