CYB5R2 (NM_016229) Human Tagged ORF Clone
CAT#: RG217261
- TrueORF®
CYB5R2 (tGFP-tagged) - Human cytochrome b5 reductase 2 (CYB5R2)
ORF Plasmid: DDK
"NM_016229" in other vectors (4)
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CNY 4,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B5R.2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217261 representing NM_016229
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCAGGAGGAGAGAGCCAATCACCTTACAGGACCCTGAAGCCAAGTACCCGCTGCCCTTGATTG AGAAAGAGAAAATCAGCCACAACACCCGGAGGTTCCGCTTTGGACTGCCTTCGCCGGACCATGTCTTAGG GCTTCCTGTAGGTAACTATGTCCAGCTCTTGGCAAAAATCGATAATGAATTGGTGGTCAGGGCTTACACC CCTGTCTCCAGTGATGATGACAGAGGCTTTGTGGACCTAATTATAAAGATCTACTTCAAAAATGTACACC CCCAATATCCTGAAGGTGGGAAGATGACTCAGTATTTGGAGAACATGAAAATCGGGGAGACCATCTTTTT TCGAGGGCCAAGGGGACGCTTGTTTTACCATGGGCCAGGGAATCTTGGAATCAGACCAGACCAGACGAGT GAGCCTAAAAAAACACTGGCCGATCACCTGGGAATGATTGCTGGGGGCACAGGCATCACACCCATGTTGC AGCTCATTCGCCACATCACCAAGGACCCCAGTGACAGGACCAGGATGTCCCTCATCTTTGCCAACCAGGT CAGTTCCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217261 representing NM_016229
Red=Cloning site Green=Tags(s) MNSRRREPITLQDPEAKYPLPLIEKEKISHNTRRFRFGLPSPDHVLGLPVGNYVQLLAKIDNELVVRAYT PVSSDDDRGFVDLIIKIYFKNVHPQYPEGGKMTQYLENMKIGETIFFRGPRGRLFYHGPGNLGIRPDQTS EPKKTLADHLGMIAGGTGITPMLQLIRHITKDPSDRTRMSLIFANQVSSC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016229 |
ORF Size | 570 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016229.2, NP_057313.2 |
RefSeq Size | 1341 bp |
RefSeq ORF | 831 bp |
Locus ID | 51700 |
UniProt ID | Q6BCY4 |
Domains | NAD_binding_1, FAD_binding_6 |
Protein Families | Druggable Genome |
Gene Summary | The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217261 | CYB5R2 (Myc-DDK-tagged)-Human cytochrome b5 reductase 2 (CYB5R2) |
CNY 3,656.00 |
|
RC217261L3 | Lenti-ORF clone of CYB5R2 (Myc-DDK-tagged)-Human cytochrome b5 reductase 2 (CYB5R2) |
CNY 5,890.00 |
|
RC217261L4 | Lenti-ORF clone of CYB5R2 (mGFP-tagged)-Human cytochrome b5 reductase 2 (CYB5R2) |
CNY 5,890.00 |
|
SC114350 | CYB5R2 (untagged)-Human cytochrome b5 reductase 2 (CYB5R2) |
CNY 2,400.00 |