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DDX11 (NM_030653) Human Tagged ORF Clone
CAT＃: RG219308
- TrueORF®
DDX11 (tGFP-tagged) - Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 1
ORF Plasmid: DDK
"NM_030653" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CHL1; CHLR1; KRG2; WABS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219308 representing NM_030653
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGG AAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGG CACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGT GAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTC TGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTT TGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGA GAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAG AAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCT GGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTG GCGAGCAGAGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTATTACTGTAGTC GGACACACTCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAGGATGTTCGGCT GGTCTCCCTTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGTTCTGTGCAGCTT ATCAACGACCGCTGCGTGGACATGCAGAGAAGCAGGCATGAGAAGAAGAAAGGAGCTGAGGAGGAGAAGC CAAAGAGGAGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAGATGGGCCTTCTCCG GGATGAGGCCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAGGAGGCCCGGGCCTGT CCCTATTACGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCCTATCAGATGCTGCTGC ATGCGGCCACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATCATCGACGAGGCGCACAA CCTGATCGACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAGCTCTGCCAGGCCCATTCC CAGCTGCTGCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAACCTGATGTACCTGAAGCAGA TCCTGTATTTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAGCAAAATCCCAATACACAGAG TCTGTCACAGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTCCAGAGCCAGATCGACAACATC AACCTGTTCAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGAAAGCTCTTTGGATTCACTGAAC GGTACGGAGCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGGTTTCAGCAATTCCTGCAGAGCCT GCAGCCCAGGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGTCAGGCCAGCACCCTGCGACCAGCT TCTCCACTGATGCACATTGAAGGCTTCCTGGCAGCTCTCACTACGGCCAACCAGGACGGCAGGGTCATCC TGAGCCGCCAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTGCTCCTGAATCCAGCTGTGCACTTTGC CCAAGTGGTGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGTACCATGCAGCCGGTGTCTGACTTCCGG CAGCAGCTGCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTGGTGGAGTTTTCCTGTGGTCACGTGATCC CTCCAGACAACATCCTGCCCCTTGTCATCTGCAGCGGGATCTCCAACCAGCCGCTGGAATTCACGTTCCA GAAAAGAGAGCTGCCTCAGATGATGGACGAGGTGGGTCGCATTCTCTGTAACCTGTGCGGTGTGGTTCCT GGAGGGGTGGTCTGTTTCTTCCCCTCCTACGAGTACCTGCGCCAGGTCCATGCCCACTGGGAGAAGGGTG GCCTGCTGGGCCGTCTGGCTGCCAGGAAGAAGATATTCCAGGAACCTAAGAGCGCACACCAGGTGGAGCA GGTGCTGCTGGCATATTCCAGGTGCATCCAGGCCTGTGGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTG CTCCTCTCTGTGGTTGGAGGAAAGATGAGTGAAGGGATCAACTTCTCTGACAACCTAGGCCGGTGTGTGG TGATGGTGGGCATGCCCTTCCCCAACATCAGGTCTGCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCA AACCCTCCCCAGAGCCCCCGGCCAGGCACCCCCAGGGAAGGCTCTGGTGGAGAACCTGTGCATGAAGGCC GTCAACCAGTCCATAGGCAGGGCCATCAGGCACCAGAAGGATTTTGCCAGCATAGTGCTCCTGGACCAGC GATATGCCCGGCCCCCTGTCCTGGCCAAGCTGCCGGCCTGGATCCGAGCCCGTGTGGAGGTCAAAGCTAC CTTTGGCCCCGCCATTGCTGCTGTGCAGAAGTTTCACCGGGAGAAGTCGGCCTCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219308 representing NM_030653
Red=Cloning site Green=Tags(s) MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKR EEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQR EERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKV ASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQL INDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARAC PYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHS QLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNI NLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPA SPLMHIEGFLAALTTANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFR QQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVP GGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGAL LLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLPRAPGQAPPGKALVENLCMKA VNQSIGRAIRHQKDFASIVLLDQRYARPPVLAKLPAWIRARVEVKATFGPAIAAVQKFHREKSASS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030653 |
| ORF Size | 2718 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030653.2, NP_085911.1 |
| RefSeq Size | 3875 bp |
| RefSeq ORF | 2721 bp |
| Locus ID | 1663 |
| Domains | DEXDc2, HELICc2 |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219308 | DDX11 (Myc-DDK-tagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 1 |
CNY 5,488.00 |
|
| RC219308L3 | Lenti-ORF clone of DDX11 (Myc-DDK-tagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 1 |
CNY 9,030.00 |
|
| RC219308L4 | Lenti-ORF clone of DDX11 (mGFP-tagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 1 |
CNY 9,030.00 |
|
| SC109150 | DDX11 (untagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 1 |
CNY 6,640.00 |
