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MYH14 (NM_001077186) Human Tagged ORF Clone
CAT＃: RG221497
- TrueORF®
MYH14 (tGFP-tagged) - Human myosin, heavy chain 14, non-muscle (MYH14), transcript variant 1
ORF Plasmid: DDK
"NM_001077186" in other vectors (2)
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CNY 20,900.00
货期*
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DFNA4; DFNA4A; FP17425; MHC16; MYH17; myosin; NMHC-II-C; NMHC II-C; PNMHH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221497 representing NM_001077186
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGTGACCATGTCGGTGCCCGGGCGGAAGGCGCCCCCCAGGCCGGGCCCAGTGCCCGAGGCGG CCCAGCCGTTCCTGTTCACGCCCCGCGGGCCCAGCGCGGGTGGCGGGCCTGGCTCGGGCACCTCCCCGCA GGTGGAGTGGACGGCCCGGCGTCTCGTGTGGGTGCCTTCGGAGCTTCACGGGTTCGAGGCGGCGGCGCTG CGGGACGAAGGCGAGGAGGAGGCGGAGGTGGAGCTGGCGGAGAGCGGGAGGCGGCTGCGACTGCCGCGGG ACCAGATCCAGCGCATGAACCCGCCCAAGTTCAGCAAGGCCGAGGACATGGCCGAGCTGACCTGCCTCAA CGAGGCCTCGGTCCTGCACAACCTCCGGGAGCGGTACTACTCCGGCCTCATCTACACGTACTCCGGCCTT TTCTGTGTGGTCATCAACCCGTACAAGCAGCTTCCCATCTACACAGAAGCCATTGTGGAGATGTACCGGG GCAAGAAGCGCCACGAGGTGCCACCCCACGTGTACGCAGTGACCGAGGGGGCCTATCGGAGCATGCTGCA GGATCGTGAGGACCAGTCCATTCTCTGCACTGGAGAGTCTGGAGCTGGGAAGACGGAAAACACCAAGAAG GTCATCCAGTACCTCGCCCACGTGGCGTCGTCTCCAAAGGGCAGGAAGGAGCCGGGTGTCCCCGCCTCCG TCAGCACCGTGTCTTATGGTGAGCTGGAGCGGCAGCTGCTTCAGGCCAACCCCATCCTAGAGGCCTTTGG CAATGCCAAGACAGTGAAGAATGACAACTCCTCCCGATTCGGCAAATTCATCCGCATCAACTTTGATGTT GCCGGGTACATCGTGGGCGCCAACATTGAGACCTACCTGCTGGAGAAGTCGCGGGCCATCCGCCAGGCCA AGGACGAGTGCAGCTTCCACATCTTCTACCAGCTGCTGGGGGGCGCTGGAGAGCAGCTCAAAGCCGACCT CCTCCTCGAGCCCTGCTCCCACTACCGGTTCCTGACCAACGGGCCGTCATCCTCTCCCGGCCAGGAGCGG GAACTCTTCCAGGAGACGCTGGAGTCGCTGCGGGTCCTGGGATTCAGCCACGAGGAAATCATCTCCATGC TGCGGATGGTCTCAGCAGTTCTCCAGTTTGGCAACATTGCCTTGAAGAGAGAACGGAACACCGATCAAGC CACCATGCCTGACAACACAGCTGCACAGAAGCTCTGCCGCCTCTTGGGACTGGGGGTGACGGATTTCTCC CGAGCCTTGCTCACCCCTCGCATCAAAGTTGGCCGAGACTATGTGCAGAAAGCCCAGACTAAGGAACAGG CTGACTTCGCGCTGGAGGCCCTGGCCAAGGCCACCTACGAGCGCCTCTTCCGCTGGCTGGTTCTGCGCCT CAACCGGGCCTTGGACCGCAGCCCCCGCCAAGGCGCCTCCTTCCTGGGCATCCTGGACATCGCGGGCTTT GAGATCTTCCAGCTGAACTCCTTCGAGCAGCTCTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCT TCAACCACACCATGTTCGTGCTGGAGCAGGAGGAGTACCAGCGTGAGGGCATCCCCTGGACCTTCCTCGA CTTTGGCCTCGACCTGCAGCCCTGCATCGACCTCATCGAGCGGCCGGCCAACCCCCCTGGACTCCTGGCC CTGCTGGATGAGGAGTGCTGGTTCCCGAAGGCCACAGACAAGTCGTTTGTGGAGAAGGTAGCCCAGGAGC AGGGCGGCCACCCCAAGTTCCAGCGGCCGAGGCACCTGCGGGATCAGGCCGACTTCAGTGTTCTCCACTA CGCGGGCAAGGTCGACTACAAGGCCAACGAGTGGCTGATGAAAAACATGGACCCTCTGAATGACAACGTC GCAGCCTTGCTCCACCAGAGCACAGACCGGCTGACGGCAGAGATCTGGAAAGACGTGGAGGGCATCGTGG GGCTGGAACAGGTGAGCAGCCTGGGCGACGGCCCACCAGGTGGCCGCCCCCGTCGGGGTATGTTCCGGAC AGTGGGACAGCTCTACAAGGAGTCCCTGAGCCGCCTCATGGCCACACTCAGCAACACCAACCCCAGTTTT GTCCGCTGCATTGTCCCCAACCACGAGAAGAGGGCCGGGAAGCTGGAGCCACGGCTGGTGCTGGACCAGC TTCGCTGCAACGGGGTCCTGGAGGGCATCCGCATCTGTCGCCAGGGCTTCCCCAACCGCATCCTCTTCCA GGAGTTCCGGCAGCGATACGAGATCCTGACACCCAATGCCATCCCCAAGGGCTTCATGGATGGGAAGCAG GCCTGTGAAAAGATGATCCAGGCGCTGGAACTGGACCCCAACCTCTACCGCGTGGGACAGAGCAAGATCT TCTTCCGGGCTGGGGTCCTGGCCCAGCTGGAAGAGGAGCGAGACCTGAAGGTCACCGACATCATCGTCTC CTTCCAGGCAGCTGCCCGGGGATACCTGGCTCGCAGGGCCTTCCAGAAGCGCCAGCAGCAGCAGAGCGCC CTGAGGGTGATGCAGCGGAACTGCGCGGCCTACCTCAAGCTGAGACACTGGCAGTGGTGGCGGCTGTTTA CCAAGGTGAAGCCACTGCTGCAGGTGACGCGGCAGGATGAGGTGCTGCAGGCACGGGCCCAGGAGCTGCA GAAAGTGCAGGAGCTACAGCAGCAGAGCGCCCGCGAAGTTGGGGAGCTCCAGGGCCGAGTGGCACAGCTG GAAGAGGAGCGCGCCCGCCTGGCAGAGCAATTGCGAGCAGAGGCAGAACTGTGTGCAGAGGCCGAGGAGA CGCGGGGGAGGCTGGCAGCCCGCAAGCAGGAGCTGGAGCTGGTGGTGTCAGAGCTGGAGGCTCGCGTGGG CGAGGAGGAGGAGTGCAGCCGTCAAATGCAAACCGAGAAGAAGAGGCTGCAGCAGCACATACAGGAGCTA GAGGCCCACCTTGAGGCTGAGGAGGGTGCGCGGCAGAAGCTGCAGCTGGAGAAGGTGACGACAGAGGCAA AAATGAAGAAATTTGAAGAGGACCTGCTGCTCCTGGAAGACCAGAATTCCAAGCTGAGCAAGGAGCGGAA GCTGCTGGAAGATCGTCTGGCCGAGTTCTCATCCCAGGCAGCTGAGGAGGAGGAGAAGGTCAAGAGCCTC AATAAGCTACGGCTCAAATATGAGGCCACAATCGCAGACATGGAGGACCGCCTACGGAAGGAGGAGAAGG GTCGCCAGGAGCTGGAGAAGCTGAAGCGGAGGCTGGATGGGGAGAGCTCAGAGCTGCAGGAGCAGATGGT GGAGCAGCAACAGCGGGCAGAGGAGCTGCGGGCCCAGCTGGGCCGGAAGGAGGAGGAGCTGCAGGCTGCC CTGGCCAGGGCAGAAGACGAGGGTGGGGCCCGGGCCCAGCTGCTGAAATCCCTGCGGGAGGCTCAAGCAG CCCTGGCCGAGGCCCAGGAGGACCTGGAGTCTGAGCGTGTGGCCAGGACCAAGGCGGAGAAGCAGCGCCG GGACCTGGGCGAGGAGCTGGAGGCGCTGCGGGGCGAGCTGGAGGACACGCTGGACTCCACCAACGCACAG CAGGAGCTCCGGTCCAAGAGGGAACAGGAGGTGACGGAGCTGAAGAAGACTCTGGAGGAGGAGACTCGCA TCCACGAGGCGGCAGTGCAGGAGCTGAGGCAGCGCCACGGCCAGGCCCTGGGGGAGCTGGCGGAGCAGCT GGAGCAGGCCCGGAGGGGCAAAGGTGCATGGGAGAAGACCCGGCTGGCCCTGGAGGCCGAGGTGTCCGAG CTGCGGGCAGAACTGAGCAGCCTGCAGACTGCACGTCAGGAGGGTGAGCAGCGGAGGCGCCGCCTGGAGT TACAGCTGCAGGAGGTGCAGGGCCGGGCTGGTGATGGGGAGAGGGCACGAGCGGAGGCTGCTGAGAAGCT GCAGCGAGCCCAGGCTGAACTGGAGAATGTGTCTGGGGCGCTGAACGAGGCTGAGTCCAAAACCATCCGT CTTAGCAAGGAGCTGAGCAGCACAGAAGCCCAGCTGCACGATGCCCAGGAGCTGCTGCAGGAGGAGACCA GGGCGAAATTGGCCTTGGGGTCCCGGGTGCGAGCCATGGAGGCTGAGGCAGCCGGGCTGCGTGAGCAGCT GGAGGAGGAGGCAGCTGCCAGGGAACGGGCGGGCCGTGAACTGCAGACTGCCCAGGCCCAGCTTTCCGAG TGGCGGCGGCGCCAGGAGGAGGAGGCAGGGGCACTGGAGGCAGGGGAGGAGGCACGGCGCCGGGCAGCCC GGGAGGCCGAGGCCCTGACCCAGCGCCTGGCAGAAAAGACAGAGACCGTGGATCGGCTGGAGCGGGGCCG CCGCCGGCTGCAGCAGGAGCTGGACGACGCCACCATGGACCTGGAGCAGCAGCGGCAGCTTGTGAGCACC CTGGAGAAGAAGCAGCGCAAGTTTGACCAGCTTCTGGCAGAGGAGAAGGCAGCTGTACTTCGGGCAGTGG AGGAACGTGAGCGGGCCGAGGCAGAGGGCCGGGAGCGTGAGGCTCGGGCCCTGTCACTGACACGGGCACT GGAGGAGGAGCAGGAGGCACGTGAGGAGCTGGAGCGGCAGAACCGGGCCCTGCGGGCTGAGCTGGAGGCA CTGCTGAGCAGCAAGGATGACGTCGGCAAGAGCGTGCATGAGCTGGAACGAGCCTGCCGGGTAGCAGAAC AGGCAGCCAATGATCTGCGAGCACAGGTGACAGAACTGGAGGATGAGCTGACAGCGGCCGAGGATGCCAA GCTGCGTCTGGAGGTGACTGTGCAGGCTCTCAAGACTCAGCATGAGCGTGACCTGCAGGGCCGTGATGAG GCTGGTGAAGAGAGGCGGAGGCAGCTGGCCAAGCAGCTGAGAGATGCAGAGGTGGAGCGGGATGAGGAGC GGAAGCAGCGCACTCTGGCCGTGGCTGCCCGCAAGAAGCTGGAGGGAGAGCTGGAGGAGCTGAAGGCTCA GATGGCCTCTGCCGGCCAGGGCAAGGAGGAGGCGGTGAAGCAGCTTCGCAAGATGCAGGCCCAGATGAAG GAGCTATGGCGGGAGGTGGAGGAGACACGCACCTCCCGGGAGGAGATCTTCTCCCAGAATCGGGAAAGTG AAAAGCGCCTCAAGGGCCTGGAGGCTGAGGTGCTGCGGCTGCAGGAGGAACTGGCCGCCTCGGACCGTGC TCGGCGGCAGGCCCAGCAGGACCGGGATGAGATGGCAGATGAGGTGGCCAATGGTAACCTTAGCAAGGCA GCCATTCTGGAGGAGAAGCGTCAGCTGGAGGGGCGCCTGGGGCAGTTGGAGGAAGAGCTGGAGGAGGAGC AGAGCAACTCGGAGCTGCTCAATGACCGCTACCGCAAGCTGCTCCTGCAGGTAGAGTCACTGACCACAGA GCTGTCAGCTGAGCGCAGTTTCTCAGCCAAGGCAGAGAGCGGGCGGCAGCAGCTGGAACGGCAGATCCAG GAGCTACGGGGACGCCTGGGTGAGGAGGATGCTGGGGCCCGTGCCCGCCACAAGATGACCATTGCTGCCC TTGAGTCTAAGTTGGCCCAGGCTGAGGAGCAGCTAGAGCAAGAGACCAGAGAGCGCATCCTCTCTGGAAA GCTGGTGCGCAGAGCTGAGAAGCGGCTTAAAGAGGTGGTGCTCCAGGTGGAGGAGGAGCGGAGGGTGGCT GACCAGCTCCGGGACCAGCTGGAGAAGGGAAACCTTCGAGTCAAGCAGCTGAAGCGGCAGCTGGAGGAGG CCGAGGAGGAGGCATCCCGGGCTCAGGCCGGCCGCCGGAGGCTGCAGCGTGAGCTGGAAGATGTCACAGA GTCGGCCGAGTCCATGAACCGTGAAGTGACCACACTGAGGAACCGGCTTCGACGCGGCCCCCTCACCTTC ACCACCCGCACGGTGCGCCAGGTCTTCCGACTAGAGGAGGGCGTGGCATCCGACGAGGAGGCAGAGGAAG CACAGCCTGGGTCTGGGCCATCCCCGGAGCCTGAGGGGTCCCCACCAGCCCACCCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221497 representing NM_001077186
Red=Cloning site Green=Tags(s) MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPSELHGFEAAAL RDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGL FCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKK VIQYLAHVASSPKGRKEPGVPASVSTVSYGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDV AGYIVGANIETYLLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQER ELFQETLESLRVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFS RALLTPRIKVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGF EIFQLNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLA LLDEECWFPKATDKSFVEKVAQEQGGHPKFQRPRHLRDQADFSVLHYAGKVDYKANEWLMKNMDPLNDNV AALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSF VRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFPNRILFQEFRQRYEILTPNAIPKGFMDGKQ ACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEERDLKVTDIIVSFQAAARGYLARRAFQKRQQQQSA LRVMQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVLQARAQELQKVQELQQQSAREVGELQGRVAQL EEERARLAEQLRAEAELCAEAEETRGRLAARKQELELVVSELEARVGEEEECSRQMQTEKKRLQQHIQEL EAHLEAEEGARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSKLSKERKLLEDRLAEFSSQAAEEEEKVKSL NKLRLKYEATIADMEDRLRKEEKGRQELEKLKRRLDGESSELQEQMVEQQQRAEELRAQLGRKEEELQAA LARAEDEGGARAQLLKSLREAQAALAEAQEDLESERVARTKAEKQRRDLGEELEALRGELEDTLDSTNAQ QELRSKREQEVTELKKTLEEETRIHEAAVQELRQRHGQALGELAEQLEQARRGKGAWEKTRLALEAEVSE LRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIR LSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSE WRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTETVDRLERGRRRLQQELDDATMDLEQQRQLVST LEKKQRKFDQLLAEEKAAVLRAVEERERAEAEGREREARALSLTRALEEEQEAREELERQNRALRAELEA LLSSKDDVGKSVHELERACRVAEQAANDLRAQVTELEDELTAAEDAKLRLEVTVQALKTQHERDLQGRDE AGEERRRQLAKQLRDAEVERDEERKQRTLAVAARKKLEGELEELKAQMASAGQGKEEAVKQLRKMQAQMK ELWREVEETRTSREEIFSQNRESEKRLKGLEAEVLRLQEELAASDRARRQAQQDRDEMADEVANGNLSKA AILEEKRQLEGRLGQLEEELEEEQSNSELLNDRYRKLLLQVESLTTELSAERSFSAKAESGRQQLERQIQ ELRGRLGEEDAGARARHKMTIAALESKLAQAEEQLEQETRERILSGKLVRRAEKRLKEVVLQVEEERRVA DQLRDQLEKGNLRVKQLKRQLEEAEEEASRAQAGRRRLQRELEDVTESAESMNREVTTLRNRLRRGPLTF TTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGSPPAHPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077186 |
| ORF Size | 6009 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077186.2 |
| RefSeq Size | 6831 bp |
| RefSeq ORF | 6012 bp |
| Locus ID | 79784 |
| UniProt ID | Q7Z406 |
| Protein Pathways | Regulation of actin cytoskeleton, Tight junction, Viral myocarditis |
| Gene Summary | This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
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