United States
Germany
Japan
United Kingdom
China
APPBP1 (NAE1) (NM_001018159) Human Tagged ORF Clone
CAT＃: RG221596
- TrueORF®
NAE1 (tGFP-tagged) - Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2
ORF Plasmid: DDK
"NM_001018159" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A-116A10.1; APPBP1; HPP1; ula-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221596 representing NM_001018159
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTCAGCAAACAAAAACAAATGAAGCCAGGTTGTGGGGTGATCATGGGCAAGAGGCTTTAGAAT CTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGAAATTCTTAAAAACTTGGTACTACCAGG TATTGGTTCGTTTACAATTATTGATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTT CAAAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTACAAGAATTAAATAGCGATG TCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTT TACTGTTGTAGTTGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCTGGAATTCC CAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTTATATGAGGATCATTATAAAAGAACATC CAGTAATAGAATCTCATCCAGATAATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAG AGAACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGTCATACTCCATGGATTGTG ATCATAGCTAAATATTTAGCACAGTGGTATAGTGAAACAAATGGACGAATACCTAAAACGTATAAAGAAA AAGAGGACTTCAGAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAAGATGAAGA GAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAATACAACTCAGATCCCAAGCAGTATTGAA GATATATTTAATGATGATCGCTGCATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTG CCTTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATATGAT TGCAGATTCAGGCAAATATATAAAACTGCAAAACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCT GTGGGTAATCATGTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAAT TAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAGATGTCGATCCTTAGCTGAAGAATATGG TTTGGATACAATTAACAAGGATGAAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTAC TTAATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAGGAGTATCTAACTATCAAG TTGAAGAAGATATAGGAAAGTTGAAGTCTTGTCTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAAT GGTGAAAGATGATTATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTGCTGCATTC TTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACCAAACAATTTGTAATTTTTAATAATACTT ACATTTACAGTGGCATGTCACAAACTTCAGCAACTTTCCAGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221596 representing NM_001018159
Red=Cloning site Green=Tags(s) MDAQQTKTNEARLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFL QRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNS QIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIV IIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIE DIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAA VGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLY LMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAF LGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001018159 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001018159.1, NP_001018169.1 |
| RefSeq Size | 1918 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 8883 |
| UniProt ID | Q13564 |
| Protein Pathways | Alzheimer's disease |
| Gene Summary | The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221596 | NAE1 (Myc-DDK-tagged)-Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2 |
CNY 3,936.00 |
|
| RC221596L3 | Lenti-ORF clone of NAE1 (Myc-DDK-tagged)-Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2 |
CNY 6,460.00 |
|
| RC221596L4 | Lenti-ORF clone of NAE1 (mGFP-tagged)-Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2 |
CNY 6,460.00 |
|
| SC302200 | NAE1 (untagged)-Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2 |
CNY 8,460.00 |
