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Collagen V (COL5A3) (NM_015719) Human Tagged ORF Clone
CAT＃: RG222823
- TrueORF®
COL5A3 (tGFP-tagged) - Human collagen, type V, alpha 3 (COL5A3)
ORF Plasmid: DDK
"NM_015719" in other vectors (2)
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CNY 19,100.00
货期*
9周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222823 representing NM_015719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACCGCCGGGACCTGGGCCAGCCGCGGGCCGGTCTCTGCCTGCTCCTGGCCGCGCTGCAGCTTC TGCCGGGGACGCAGGCCGATCCTGTGGATGTCCTGAAGGCCCTGGGTGTGCAGGGAGGCCAGGCTGGGGT CCCCGAGGGGCCTGGCTTCTGTCCCCAGAGGACTCCAGAGGGTGACCGGGCATTCAGAATTGGCCAGGCC AGCACGCTCGGCATCCCCACGTGGGAACTCTTTCCAGAAGGCCACTTTCCTGAGAACTTCTCCTTGCTGA TCACCTTGCGGGGACAGCCAGCCAATCAGTCTGTCCTGCTGTCCATTTATGATGAAAGGGGTGCCCGGCA GTTGGGCCTGGCACTGGGGCCAGCGCTGGGTCTCCTAGGTGACCCCTTCCGCCCCCTCCCCCAGCAGGTC AACCTCACAGATGGCAGGTGGCACCGTGTGGCCGTCAGCATAGATGGTGAGATGGTGACCCTGGTAGCTG ACTGTGAAGCTCAGCCCCCTGTTTTGGGCCATGGCCCCCGCTTCATCAGCATAGCTGGACTCACTGTGCT GGGGACCCAGGACCTTGGGGAAAAGACTTTCGAGGGAGACATTCAGGAGCTGCTGATAAGCCCAGATCCT CAGGCTGCCTTCCAGGCTTGTGAGCGGTACCTCCCCGACTGTGACAACCTGGCACCGGCAGCCACAGTGG CTCCCCAGGGTGAACCAGAAACCCCTCGTCCTCGGCGGAAGGGGAAGGGAAAAGGGAGGAAGAAAGGGCG AGGTCGCAAGGGGAAGGGCAGGAAAAAGAACAAGGAAATTTGGACCTCAAGTCCACCTCCTGACTCCGCA GAGAACCAGACCTCCACTGACATCCCCAAGACAGAGACTCCAGCTCCAAATCTGCCTCCGACCCCCACGC CTTTGGTCGTCACCTCCACTGTGACTACTGGACTCAATGCCACGATCCTAGAGGGGAGCTTGGACCCTGA CAGTGGAACCGAGCTGGGGACCCTGGAGACCAAGGCAGCCAGGGAGGATGAAGAAGGAGATGATTCCACC ATGGGCCCTGACTTCCGGGCAGCAGAATATCCATCTCGGACTCAGTTCCAGATCTTTCCTGGTGCTGGAG AGAAAGGAGCAAAAGGAGAGCCCGCAGTGATTGAAAAGGGGCAGCAGTTTGAGGGACCTCCAGGAGCCCC AGGACCCCAAGGGGTGGTTGGCCCCTCAGGCCCTCCCGGCCCCCCAGGATTCCCTGGCGACCCTGGTCCA CCGGGCCCTGCTGGCCTCCCAGGAATCCCCGGCATTGATGGGATCCGAGGCCCACCGGGCACTGTGATCA TGATGCCGTTCCAGTTTGCAGGCGGCTCCTTTAAAGGCCCCCCAGTCTCATTCCAGCAGGCCCAGGCTCA GGCAGTTCTGCAGCAGACTCAGCTCTCTATGAAAGGCCCCCCTGGTCCAGTGGGGCTCACTGGGCGCCCA GGCCCTGTGGGTCTCCCCGGGCATCCAGGTCTGAAAGGAGAGGAGGGAGCAGAAGGGCCACAGGGTCCCC GAGGCCTGCAGGGACCTCATGGACCCCCTGGCCGAGTGGGCAAGATGGGCCGCCCTGGAGCAGATGGAGC TCGGGGCCTCCCAGGGGACACTGGACCTAAGGGTGATCGTGGCTTCGATGGCCTCCCTGGGCTGCCTGGT GAGAAGGGCCAAAGGGGTGACTTTGGCCATGTGGGGCAACCCGGTCCCCCAGGAGAGGATGGTGAGAGGG GAGCAGAGGGACCTCCAGGGCCCACTGGCCAGGCTGGGGAGCCGGGTCCACGAGGACTGCTTGGCCCCAG AGGCTCTCCTGGCCCCACGGGTCGCCCGGGTGTGACTGGAATTGATGGTGCTCCTGGTGCCAAAGGCAAT GTGGGTCCTCCAGGAGAACCAGGCCCTCCGGGACAGCAGGGAAACCATGGGTCCCAGGGACTCCCCGGTC CCCAGGGACTCATTGGCACTCCTGGGGAGAAGGGTCCCCCTGGAAACCCAGGAATTCCAGGCCTCCCAGG ATCCGATGGCCCTCTGGGTCACCCAGGACATGAGGGCCCCACGGGAGAGAAAGGGGCTCAGGGTCCACCA GGGTCGGCAGGCCCTCCGGGCTATCCTGGACCTCGGGGAGTGAAGGGCACTTCAGGCAACCGGGGCCTCC AGGGGGAGAAAGGCGAGAAGGGAGAGGACGGCTTCCCAGGCTTCAAGGGCGATGTGGGGCTCAAAGGTGA TCAGGGGAAACCCGGAGCTCCAGGTCCCCGGGGAGAGGATGGTCCTGAGGGGCCGAAGGGGCAGGCGGGG CAGGCTGGCGAGGAGGGGCCCCCAGGCTCAGCTGGGGAGAAGGGCAAGCTTGGGGTGCCAGGCCTCCCAG GTTATCCAGGACGCCCTGGACCTAAGGGATCTATTGGATTTCCCGGTCCCCTGGGACCCATAGGAGAGAA AGGGAAGTCGGGAAAGACAGGGCAGCCAGGCCTGGAAGGAGAGCGGGGACCACCAGGTTCCCGTGGAGAG AGGGGGCAACCGGGTGCCACAGGGCAACCAGGCCCCAAGGGCGATGTGGGCCAGGATGGAGCCCCTGGGA TCCCTGGAGAAAAGGGCCTCCCTGGTCTGCAAGGCCCTCCAGGATTCCCTGGGCCAAAGGGCCCCCCTGG TCACCAAGGTAAAGATGGGCGACCAGGGCACCCTGGACAGAGAGGAGAACTGGGCTTCCAAGGTCAGACA GGCCCGCCTGGACCAGCTGGTGTCTTAGGCCCTCAGGGAAAGACAGGAGAAGTGGGACCTCTAGGTGAAA GGGGGCCTCCAGGCCCCCCTGGACCTCCTGGTGAACAAGGTCTTCCTGGCCTGGAAGGCAGAGAGGGGGC CAAGGGGGAACTGGGACCACCAGGACCCCTTGGGAAAGAAGGGCCAGCTGGACTCAGGGGCTTTCCCGGC CCCAAAGGGGGCCCTGGGGACCCGGGACCTACTGGCTTAAAGGGTGATAAGGGCCCCCCAGGGCCTGTGG GGGCCAATGGCTCCCCTGGTGAGCGCGGTCCTTTGGGCCCAGCAGGAGGCATTGGACTTCCTGGCCAAAG TGGCAGCGAAGGCCCCGTTGGCCCTGCAGGCAAGAAGGGGTCCCGGGGAGAACGTGGCCCCCCTGGCCCC ACTGGCAAAGATGGGATCCCAGGGCCCCTGGGGCCTCTGGGACCCCCTGGAGCTGCTGGGCCTTCTGGCG AGGAAGGGGACAAGGGGGATGTGGGTGCCCCCGGACACAAGGGGAGTAAAGGCGATAAAGGAGACGCGGG CCCACCTGGACAACCAGGGATACGGGGTCCTGCAGGACACCCAGGTCCCCCGGGAGCAGACGGGGCTCAG GGGCGCCGGGGACCCCCAGGCCTCTTTGGGCAGAAAGGAGATGACGGAGTCAGAGGCTTTGTGGGGGTGA TTGGCCCTCCTGGACTGCAGGGGCTGCCAGGCCCTCCGGGAGAGAAAGGGGAGGTCGGAGACGTCGGGTC CATGGGTCCCCATGGAGCTCCAGGTCCTCGGGGTCCCCAAGGCCCCACTGGATCAGAGGGCACTCCAGGG CTGCCTGGAGGAGTTGGTCAGCCAGGCGCCGTGGGTGAGAAGGGTGAGCGAGGGGACGCTGGAGACCCAG GGCCTCCAGGAGCCCCAGGCATCCCGGGGCCCAAGGGAGACATTGGTGAAAAGGGGGACTCAGGCCCATC TGGAGCTGCTGGACCCCCAGGCAAGAAAGGTCCCCCTGGAGAGGATGGAGCCAAAGGGAGCGTGGGCCCC ACGGGGCTGCCCGGAGATCTAGGGCCCCCAGGAGACCCTGGAGTTTCAGGCATAGATGGTTCCCCAGGGG AGAAGGGAGACCCTGGTGATGTTGGGGGACCGGGTCCGCCTGGAGCTTCTGGGGAGCCCGGCGCCCCCGG GCCCCCCGGCAAGAGGGGTCCTTCAGGCCACATGGGTCGAGAAGGCAGAGAAGGGGAGAAAGGTGCCAAG GGGGAGCCAGGTCCTGATGGGCCCCCAGGGAGGACGGGTCCAATGGGGGCTAGAGGGCCCCCTGGACGTG TGGGGCCTGAGGGTCTTCGAGGGATCCCTGGCCCTGTGGGTGAACCAGGCCTCCTGGGAGCCCCTGGACA GATGGGCCCTCCTGGCCCCCTGGGGCCCTCTGGCCTCCCAGGGCTGAAGGGAGACACTGGCCCCAAGGGG GAAAAGGGCCACATTGGATTGATCGGTCTCATTGGCCCCCCGGGAGAAGCTGGTGAGAAAGGAGATCAGG GGTTGCCAGGCGTGCAGGGACCCCCTGGTCCCAAGGGAGACCCTGGTCCCCCTGGTCCCATTGGCTCTCT GGGCCACCCTGGGCCCCCAGGTGTGGCGGGCCCTCTAGGACAGAAAGGCTCAAAAGGGTCTCCGGGGTCC ATGGGCCCCCGTGGAGACACTGGACCTGCAGGCCCACCAGGCCCCCCGGGTGCCCCTGCCGAGCTGCATG GGCTGCGCAGGCGCCGGCGCTTCGTCCCAGTCCCGCTTCCAGTCGTGGAGGGCGGCCTGGAGGAGGTGCT GGCCTCGCTCACATCGCTGAGCTTGGAGCTGGAGCAGCTGCGGCGTCCTCCCGGCACTGCGGAGCGCCCG GGCCTCGTGTGCCACGAGCTGCACCGCAACCACCCGCACCTGCCTGATGGGGAATACTGGATTGACCCCA ACCAGGGCTGCGCGCGGGACTCGTTCAGGGTTTTTTGCAACTTCACGGCGGGAGGAGAGACCTGCCTCTA TCCCGACAAGAAGTTTGAGATCGTGAAATTGGCCTCCTGGTCCAAGGAAAAGCCTGGAGGCTGGTATAGC ACATTCCGTCGAGGGAAGAAGTTCTCCTACGTGGACGCCGACGGGTCCCCAGTGAATGTCGTGCAGCTGA ACTTCCTGAAACTGCTGAGTGCCACAGCTCGCCAGAACTTCACCTACTCCTGCCAGAATGCAGCTGCCTG GCTGGACGAAGCCACGGGTGACTACAGCCACTCCGCCCGCTTCCTTGGCACCAATGGAGAGGAGCTGTCT TTCAACCAGACGACAGCAACCACTGTCAGCGTCCCCCAGGATGGCTGCCGGCTCCGGAAAGGACAGACGA AGACCCTTTTCGAATTCAGCTCTTCTCGAGCGGGATTTCTGCCCCTGTGGGATGTGGCGGCCACTGACTT TGGCCAGACGAACCAAAAGTTTGGGTTTGAACTGGGCCCCGTCTGCTTCAGCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222823 representing NM_015719
Red=Cloning site Green=Tags(s) MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPEGDRAFRIGQA STLGIPTWELFPEGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGLALGPALGLLGDPFRPLPQQV NLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDP QAAFQACERYLPDCDNLAPAATVAPQGEPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSA ENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILEGSLDPDSGTELGTLETKAAREDEEGDDST MGPDFRAAEYPSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGP PGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRP GPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGLPGDTGPKGDRGFDGLPGLPG EKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGPRGLLGPRGSPGPTGRPGVTGIDGAPGAKGN VGPPGEPGPPGQQGNHGSQGLPGPQGLIGTPGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPP GSAGPPGYPGPRGVKGTSGNRGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAG QAGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGE RGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQT GPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPG PKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGP TGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQ GRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPG LPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGP TGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAK GEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKG EKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGS MGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERP GLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYS TFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELS FNQTTATTVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015719 |
| ORF Size | 5235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015719.2, NP_056534.1 |
| RefSeq Size | 6200 bp |
| RefSeq ORF | 5238 bp |
| Locus ID | 50509 |
| UniProt ID | P25940 |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008] |
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