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PDE4DIP (NM_022359) Human Tagged ORF Clone
CAT＃: RG223248
- TrueORF®
PDE4DIP (tGFP-tagged) - Human phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 3
ORF Plasmid: DDK
"NM_022359" in other vectors (4)
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CNY 5,200.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CMYA2; MMGL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223248 representing NM_022359
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGCACAGACAGCGGGTCCTGCTGCCGCCGCCGATGCGACTTTGGCTGCTGCTGTCGCGCGTCCC GCCGGGCTCACTACACGCCTTACCGGTCCGGGGACGCGACGCGAACCCCTCAGTCCCCACGGCAGACCCC GAGCCGGGAAAGACGGCGCCCGGAGCCAGCCGGGAGCTGGGCAGCAGCGGCCGAGGAGGAAGAAGCAGCT GCGGCGGCCACACCCTGGATGAGAGATTATTTTGCAGAGGATGATGGGGAGATGGTACCCAGAACGAGTC ACACAGCAGCTTTTCTTAGTGACACTAAAGATCGAGGCCCTCCAGTGCAGTCACAGATCTGGAGAAGTGG TGAAAAGGTCCCGTTTGTGCAGACATATTCCTTGAGAGCATTTGAGAAACCCCCTCAGGTACAGACCCAG GCTCTTCGAGACTTTGAGAAGCACCTCAATGACCTGAAGAAGGAGAACTTCAGCCTCAAGCTGCGCATCT ACTTCCTGGAGGAGCGCATGCAACAGAAGTATGAGGCCAGCCGGGAGGACATCTACAAGCGGAACATTGA GCTGAAGGTTGAAGTGGAGAGCTTGAAACGAGAACTCCAGGACAAGAAACAGCATCTGGATAAAACATGG GCTGATGTGGAGAATCTCAACAGTCAGAATGAAGCTGAGCTCCGACGCCAGTTTGAGGAGCGACAGCAGG AGACGGAGCATGTTTATGAGCTCTTGGAGAATAAGATCCAGCTTCTGCAGGAGGAATCCAGGCTAGCAAA GAATGAAGCTGCGCGGATGGCAGCTCTGGTGGAAGCAGAGAAGGAGTGTAACCTGGAGCTCTCAGAGAAA CTGAAGGGAGTCACCAAAAACTGGGAAGATGTACCAGGAGACCAGGTCAAGCCCGACCAATACACTGAGG CCCTGGCCCAGAGGGACAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223248 representing NM_022359
Red=Cloning site Green=Tags(s) MKGTDSGSCCRRRCDFGCCCRASRRAHYTPYRSGDATRTPQSPRQTPSRERRRPEPAGSWAAAAEEEEAA AAATPWMRDYFAEDDGEMVPRTSHTAAFLSDTKDRGPPVQSQIWRSGEKVPFVQTYSLRAFEKPPQVQTQ ALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTW ADVENLNSQNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEK LKGVTKNWEDVPGDQVKPDQYTEALAQRDK TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022359 |
| ORF Size | 930 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022359.7 |
| RefSeq Size | 1531 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 9659 |
| UniProt ID | Q5VU43 |
| Gene Summary | The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223248 | PDE4DIP (Myc-DDK-tagged)-Human phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 3 |
CNY 3,600.00 |
|
| RC223248L3 | Lenti ORF clone of Human phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223248L4 | Lenti ORF clone of Human phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| SC312905 | PDE4DIP (untagged)-Human phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 3 |
CNY 6,270.00 |
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