GAS1 (NM_002048) Human Tagged ORF Clone
CAT#: RG224804
- TrueORF®
GAS1 (tGFP-tagged) - Human growth arrest-specific 1 (GAS1)
ORF Plasmid: DDK
"NM_002048" in other vectors (6)
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CNY 4,370.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG224804 representing NM_002048
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCGCGCTGCTGGGCGGCGGCGGCGAGGCCCGCGGGGGGACAGTGCCGGGCGCCTGGCTGTGCC TGATGGCGCTGCTGCAGCTGCTGGGCTCGGCGCCGCGGGGATCGGGGCTGGCGCACGGCCGCCGCCTCAT CTGCTGGCAGGCGCTGCTGCAGTGCCAGGGGGAGCCGGAGTGCAGCTACGCCTACAACCAGTACGCCGAG GCGTGCGCGCCGGTGCTGGCGCAGCACGGCGGGGGCGACGCGCCCGGGGCCGCCGCCGCCGCTTTCCCGG CCTCGGCCGCCTCTTTCTCGTCGCGCTGGCGCTGCCCGAGTCACTGCATCTCGGCCCTCATTCAGCTCAA CCACACGCGCCGCGGGCCCGCCCTGGAGGACTGTGACTGCGCGCAGGACGAGAACTGCAAGTCCACCAAG CGCGCCATTGAGCCGTGCCTGCCCCGGACGAGCGGCGGCGGCGCGGGCGGCCCCGGCGCGGGCGGGGTCA TGGGCTGCACCGAGGCCCGGCGGCGCTGCGACCGCGACAGCCGCTGCAACCTGGCGCTGAGCCGCTACCT GACCTACTGCGGCAAAGTCTTCAACGGGCTGCGCTGCACGGACGAATGCCGCACCGTCATTGAGGACATG CTGGCTATGCCCAAGGTGGCGCTGCTCAACGACTGCGTGTGCGACGGCCTCGAGCGGCCCATCTGCGAGT CGGTCAAGGAGAACATGGCCCGCCTGTGCTTCGGCGCCGAGCTGGGCAACGGCCCCGGCAGCAGCGGCTC GGACGGGGGCCTGGACGACTACTACGATGAGGACTACGATGACGAGCAGCGCACCGGGGGCGCGGGTGGT GAGCAGCCGCTGGACGACGACGACGGCGTCCCGCACCCACCGCGCCCGGGCAGCGGCGCTGCTGCATCGG GCGGCCGCGGGGACCTGCCCTATGGGCCTGGGCGCAGGAGCAGCGGCGGCGGCGGCCGCTTGGCGCCCCG GGGCGCCTGGACCCCACTCGCCTCCATCTTGCTGCTGCTGCTTGGGCCGCTCTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224804 representing NM_002048
Red=Cloning site Green=Tags(s) MVAALLGGGGEARGGTVPGAWLCLMALLQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAE ACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTK RAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDM LAMPKVALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGG EQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002048 |
ORF Size | 1035 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002048.1, NP_002039.1 |
RefSeq Size | 2828 bp |
RefSeq ORF | 1038 bp |
Locus ID | 2619 |
UniProt ID | P54826 |
Protein Pathways | Hedgehog signaling pathway |
Gene Summary | Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Gas1 Interferes with A?PP Trafficking by Facilitating the Accumulation of Immature A?PP in Endoplasmic Reticulum-Associated Raft Subdomains
,null,
Journal of Alzheimer's Disease
,PubMed ID 21971401
[GAS1]
|
Growth arrest-specific 1 binds to and controls the maturation and processing of the amyloid-u03b2 precursor protein
,null,
Human Molecular Genetics
,PubMed ID 21357679
[GAS1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224804 | GAS1 (Myc-DDK-tagged)-Human growth arrest-specific 1 (GAS1) |
CNY 3,656.00 |
|
RC224804L1 | Lenti ORF clone of Human growth arrest-specific 1 (GAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC224804L2 | Lenti ORF clone of Human growth arrest-specific 1 (GAS1), mGFP tagged |
CNY 5,890.00 |
|
RC224804L3 | Lenti ORF clone of Human growth arrest-specific 1 (GAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC224804L4 | Lenti ORF clone of Human growth arrest-specific 1 (GAS1), mGFP tagged |
CNY 5,890.00 |
|
SC303112 | GAS1 (untagged)-Human growth arrest-specific 1 (GAS1) |
CNY 3,656.00 |