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MYH14 (NM_001145809) Human Tagged ORF Clone
CAT＃: RG226697
- TrueORF®
MYH14 (tGFP-tagged) - Human myosin, heavy chain 14, non-muscle (MYH14), transcript variant 3
ORF Plasmid: DDK
"NM_001145809" in other vectors (2)
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CNY 20,900.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DFNA4; DFNA4A; FP17425; MHC16; MYH17; myosin; NMHC-II-C; NMHC II-C; PNMHH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226697 representing NM_001145809
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGTGACCATGTCGGTGCCCGGGCGGAAGGCGCCCCCCAGGCCGGGCCCAGTGCCCGAGGCGG CCCAGCCGTTCCTGTTCACGCCCCGCGGGCCCAGCGCGGGTGGCGGGCCTGGCTCGGGCACCTCCCCGCA GGTGGAGTGGACGGCCCGGCGTCTCGTGTGGGTGCCTTCGGAGCTTCACGGGTTCGAGGCGGCGGCGCTG CGGGACGAAGGCGAGGAGGAGGCGGAGGTGGAGCTGGCGGAGAGCGGGAGGCGGCTGCGACTGCCGCGGG ACCAGATCCAGCGCATGAACCCGCCCAAGTTCAGCAAGGCCGAGGACATGGCCGAGCTGACCTGCCTCAA CGAGGCCTCGGTCCTGCACAACCTCCGGGAGCGGTACTACTCCGGCCTCATCTACACGTACTCCGGCCTT TTCTGTGTGGTCATCAACCCGTACAAGCAGCTTCCCATCTACACAGAAGCCATTGTGGAGATGTACCGGG GCAAGAAGCGCCACGAGGTGCCACCCCACGTGTACGCAGTGACCGAGGGGGCCTATCGGAGCATGCTGCA GGATCGTGAGGACCAGTCCATTCTCTGCACTGGAGAGTCTGGAGCTGGGAAGACGGAAAACACCAAGAAG GTCATCCAGTACCTCGCCCACGTGGCGTCGTCTCCAAAGGGCAGGAAGGAGCCGGGTGTCCCCGCCTCCG TCAGCACCGTGTCTTATGGTGAGCTGGAGCGGCAGCTGCTTCAGGCCAACCCCATCCTAGAGGCCTTTGG CAATGCCAAGACAGTGAAGAATGACAACTCCTCCCGATTCGGCAAATTCATCCGCATCAACTTTGATGTT GCCGGGTACATCGTGGGCGCCAACATTGAGACCTACCTGCTGGAGAAGTCGCGGGCCATCCGCCAGGCCA AGGACGAGTGCAGCTTCCACATCTTCTACCAGCTGCTGGGGGGCGCTGGAGAGCAGCTCAAAGCCGACCT CCTCCTCGAGCCCTGCTCCCACTACCGGTTCCTGACCAACGGGCCGTCATCCTCTCCCGGCCAGGAGCGG GAACTCTTCCAGGAGACGCTGGAGTCGCTGCGGGTCCTGGGATTCAGCCACGAGGAAATCATCTCCATGC TGCGGATGGTCTCAGCAGTTCTCCAGTTTGGCAACATTGCCTTGAAGAGAGAACGGAACACCGATCAAGC CACCATGCCTGACAACACAGCTGCACAGAAGCTCTGCCGCCTCTTGGGACTGGGGGTGACGGATTTCTCC CGAGCCTTGCTCACCCCTCGCATCAAAGTTGGCCGAGACTATGTGCAGAAAGCCCAGACTAAGGAACAGG CTGACTTCGCGCTGGAGGCCCTGGCCAAGGCCACCTACGAGCGCCTCTTCCGCTGGCTGGTTCTGCGCCT CAACCGGGCCTTGGACCGCAGCCCCCGCCAAGGCGCCTCCTTCCTGGGCATCCTGGACATCGCGGGCTTT GAGATCTTCCAGCTGAACTCCTTCGAGCAGCTCTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCT TCAACCACACCATGTTCGTGCTGGAGCAGGAGGAGTACCAGCGTGAGGGCATCCCCTGGACCTTCCTCGA CTTTGGCCTCGACCTGCAGCCCTGCATCGACCTCATCGAGCGGCCGGCCAACCCCCCTGGACTCCTGGCC CTGCTGGATGAGGAGTGCTGGTTCCCGAAGGCCACAGACAAGTCGTTTGTGGAGAAGGTAGCCCAGGAGC AGGGCGGCCACCCCAAGTTCCAGCGGCCGAGGCACCTGCGGGATCAGGCCGACTTCAGTGTTCTCCACTA CGCGGGCAAGGTCGACTACAAGGCCAACGAGTGGCTGATGAAAAACATGGACCCTCTGAATGACAACGTC GCAGCCTTGCTCCACCAGAGCACAGACCGGCTGACGGCAGAGATCTGGAAAGACGAACATGGGGGCTTCC AGCAGTTCTCTTTCCTTGGCTCCTTCCCACCGTCGCCCCCAGGATCTGCAGAGAGGTGCAGCTCTGCTAT TTCTCCGCCAGGGGTGGAGGGCATCGTGGGGCTGGAACAGGTGAGCAGCCTGGGCGACGGCCCACCAGGT GGCCGCCCCCGTCGGGGTATGTTCCGGACAGTGGGACAGCTCTACAAGGAGTCCCTGAGCCGCCTCATGG CCACACTCAGCAACACCAACCCCAGTTTTGTCCGCTGCATTGTCCCCAACCACGAGAAGAGGGCCGGGAA GCTGGAGCCACGGCTGGTGCTGGACCAGCTTCGCTGCAACGGGGTCCTGGAGGGCATCCGCATCTGTCGC CAGGGCTTCCCCAACCGCATCCTCTTCCAGGAGTTCCGGCAGCGATACGAGATCCTGACACCCAATGCCA TCCCCAAGGGCTTCATGGATGGGAAGCAGGCCTGTGAAAAGATGATCCAGGCGCTGGAACTGGACCCCAA CCTCTACCGCGTGGGACAGAGCAAGATCTTCTTCCGGGCTGGGGTCCTGGCCCAGCTGGAAGAGGAGCGA GACCTGAAGGTCACCGACATCATCGTCTCCTTCCAGGCAGCTGCCCGGGGATACCTGGCTCGCAGGGCCT TCCAGAAGCGCCAGCAGCAGCAGAGCGCCCTGAGGGTGATGCAGCGGAACTGCGCGGCCTACCTCAAGCT GAGACACTGGCAGTGGTGGCGGCTGTTTACCAAGGTGAAGCCACTGCTGCAGGTGACGCGGCAGGATGAG GTGCTGCAGGCACGGGCCCAGGAGCTGCAGAAAGTGCAGGAGCTACAGCAGCAGAGCGCCCGCGAAGTTG GGGAGCTCCAGGGCCGAGTGGCACAGCTGGAAGAGGAGCGCGCCCGCCTGGCAGAGCAATTGCGAGCAGA GGCAGAACTGTGTGCAGAGGCCGAGGAGACGCGGGGGAGGCTGGCAGCCCGCAAGCAGGAGCTGGAGCTG GTGGTGTCAGAGCTGGAGGCTCGCGTGGGCGAGGAGGAGGAGTGCAGCCGTCAAATGCAAACCGAGAAGA AGAGGCTGCAGCAGCACATACAGGAGCTAGAGGCCCACCTTGAGGCTGAGGAGGGTGCGCGGCAGAAGCT GCAGCTGGAGAAGGTGACGACAGAGGCAAAAATGAAGAAATTTGAAGAGGACCTGCTGCTCCTGGAAGAC CAGAATTCCAAGCTGAGCAAGGAGCGGAAGCTGCTGGAAGATCGTCTGGCCGAGTTCTCATCCCAGGCAG CTGAGGAGGAGGAGAAGGTCAAGAGCCTCAATAAGCTACGGCTCAAATATGAGGCCACAATCGCAGACAT GGAGGACCGCCTACGGAAGGAGGAGAAGGGTCGCCAGGAGCTGGAGAAGCTGAAGCGGAGGCTGGATGGG GAGAGCTCAGAGCTGCAGGAGCAGATGGTGGAGCAGCAACAGCGGGCAGAGGAGCTGCGGGCCCAGCTGG GCCGGAAGGAGGAGGAGCTGCAGGCTGCCCTGGCCAGGGCAGAAGACGAGGGTGGGGCCCGGGCCCAGCT GCTGAAATCCCTGCGGGAGGCTCAAGCAGCCCTGGCCGAGGCCCAGGAGGACCTGGAGTCTGAGCGTGTG GCCAGGACCAAGGCGGAGAAGCAGCGCCGGGACCTGGGCGAGGAGCTGGAGGCGCTGCGGGGCGAGCTGG AGGACACGCTGGACTCCACCAACGCACAGCAGGAGCTCCGGTCCAAGAGGGAACAGGAGGTGACGGAGCT GAAGAAGACTCTGGAGGAGGAGACTCGCATCCACGAGGCGGCAGTGCAGGAGCTGAGGCAGCGCCACGGC CAGGCCCTGGGGGAGCTGGCGGAGCAGCTGGAGCAGGCCCGGAGGGGCAAAGGTGCATGGGAGAAGACCC GGCTGGCCCTGGAGGCCGAGGTGTCCGAGCTGCGGGCAGAACTGAGCAGCCTGCAGACTGCACGTCAGGA GGGTGAGCAGCGGAGGCGCCGCCTGGAGTTACAGCTGCAGGAGGTGCAGGGCCGGGCTGGTGATGGGGAG AGGGCACGAGCGGAGGCTGCTGAGAAGCTGCAGCGAGCCCAGGCTGAACTGGAGAATGTGTCTGGGGCGC TGAACGAGGCTGAGTCCAAAACCATCCGTCTTAGCAAGGAGCTGAGCAGCACAGAAGCCCAGCTGCACGA TGCCCAGGAGCTGCTGCAGGAGGAGACCAGGGCGAAATTGGCCTTGGGGTCCCGGGTGCGAGCCATGGAG GCTGAGGCAGCCGGGCTGCGTGAGCAGCTGGAGGAGGAGGCAGCTGCCAGGGAACGGGCGGGCCGTGAAC TGCAGACTGCCCAGGCCCAGCTTTCCGAGTGGCGGCGGCGCCAGGAGGAGGAGGCAGGGGCACTGGAGGC AGGGGAGGAGGCACGGCGCCGGGCAGCCCGGGAGGCCGAGGCCCTGACCCAGCGCCTGGCAGAAAAGACA GAGACCGTGGATCGGCTGGAGCGGGGCCGCCGCCGGCTGCAGCAGGAGCTGGACGACGCCACCATGGACC TGGAGCAGCAGCGGCAGCTTGTGAGCACCCTGGAGAAGAAGCAGCGCAAGTTTGACCAGCTTCTGGCAGA GGAGAAGGCAGCTGTACTTCGGGCAGTGGAGGAACGTGAGCGGGCCGAGGCAGAGGGCCGGGAGCGTGAG GCTCGGGCCCTGTCACTGACACGGGCACTGGAGGAGGAGCAGGAGGCACGTGAGGAGCTGGAGCGGCAGA ACCGGGCCCTGCGGGCTGAGCTGGAGGCACTGCTGAGCAGCAAGGATGACGTCGGCAAGAGCGTGCATGA GCTGGAACGAGCCTGCCGGGTAGCAGAACAGGCAGCCAATGATCTGCGAGCACAGGTGACAGAACTGGAG GATGAGCTGACAGCGGCCGAGGATGCCAAGCTGCGTCTGGAGGTGACTGTGCAGGCTCTCAAGACTCAGC ATGAGCGTGACCTGCAGGGCCGTGATGAGGCTGGTGAAGAGAGGCGGAGGCAGCTGGCCAAGCAGCTGAG AGATGCAGAGGTGGAGCGGGATGAGGAGCGGAAGCAGCGCACTCTGGCCGTGGCTGCCCGCAAGAAGCTG GAGGGAGAGCTGGAGGAGCTGAAGGCTCAGATGGCCTCTGCCGGCCAGGGCAAGGAGGAGGCGGTGAAGC AGCTTCGCAAGATGCAGGCCCAGATGAAGGAGCTATGGCGGGAGGTGGAGGAGACACGCACCTCCCGGGA GGAGATCTTCTCCCAGAATCGGGAAAGTGAAAAGCGCCTCAAGGGCCTGGAGGCTGAGGTGCTGCGGCTG CAGGAGGAACTGGCCGCCTCGGACCGTGCTCGGCGGCAGGCCCAGCAGGACCGGGATGAGATGGCAGATG AGGTGGCCAATGGTAACCTTAGCAAGGCAGCCATTCTGGAGGAGAAGCGTCAGCTGGAGGGGCGCCTGGG GCAGTTGGAGGAAGAGCTGGAGGAGGAGCAGAGCAACTCGGAGCTGCTCAATGACCGCTACCGCAAGCTG CTCCTGCAGGTAGAGTCACTGACCACAGAGCTGTCAGCTGAGCGCAGTTTCTCAGCCAAGGCAGAGAGCG GGCGGCAGCAGCTGGAACGGCAGATCCAGGAGCTACGGGGACGCCTGGGTGAGGAGGATGCTGGGGCCCG TGCCCGCCACAAGATGACCATTGCTGCCCTTGAGTCTAAGTTGGCCCAGGCTGAGGAGCAGCTAGAGCAA GAGACCAGAGAGCGCATCCTCTCTGGAAAGCTGGTGCGCAGAGCTGAGAAGCGGCTTAAAGAGGTGGTGC TCCAGGTGGAGGAGGAGCGGAGGGTGGCTGACCAGCTCCGGGACCAGCTGGAGAAGGGAAACCTTCGAGT CAAGCAGCTGAAGCGGCAGCTGGAGGAGGCCGAGGAGGAGGCATCCCGGGCTCAGGCCGGCCGCCGGAGG CTGCAGCGTGAGCTGGAAGATGTCACAGAGTCGGCCGAGTCCATGAACCGTGAAGTGACCACACTGAGGA ACCGGCTTCGACGCGGCCCCCTCACCTTCACCACCCGCACGGTGCGCCAGGTCTTCCGACTAGAGGAGGG CGTGGCATCCGACGAGGAGGCAGAGGAAGCACAGCCTGGGTCTGGGCCATCCCCGGAGCCTGAGGGGTCC CCACCAGCCCACCCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226697 representing NM_001145809
Red=Cloning site Green=Tags(s) MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPSELHGFEAAAL RDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGL FCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKK VIQYLAHVASSPKGRKEPGVPASVSTVSYGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDV AGYIVGANIETYLLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQER ELFQETLESLRVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFS RALLTPRIKVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGF EIFQLNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLA LLDEECWFPKATDKSFVEKVAQEQGGHPKFQRPRHLRDQADFSVLHYAGKVDYKANEWLMKNMDPLNDNV AALLHQSTDRLTAEIWKDEHGGFQQFSFLGSFPPSPPGSAERCSSAISPPGVEGIVGLEQVSSLGDGPPG GRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICR QGFPNRILFQEFRQRYEILTPNAIPKGFMDGKQACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEER DLKVTDIIVSFQAAARGYLARRAFQKRQQQQSALRVMQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDE VLQARAQELQKVQELQQQSAREVGELQGRVAQLEEERARLAEQLRAEAELCAEAEETRGRLAARKQELEL VVSELEARVGEEEECSRQMQTEKKRLQQHIQELEAHLEAEEGARQKLQLEKVTTEAKMKKFEEDLLLLED QNSKLSKERKLLEDRLAEFSSQAAEEEEKVKSLNKLRLKYEATIADMEDRLRKEEKGRQELEKLKRRLDG ESSELQEQMVEQQQRAEELRAQLGRKEEELQAALARAEDEGGARAQLLKSLREAQAALAEAQEDLESERV ARTKAEKQRRDLGEELEALRGELEDTLDSTNAQQELRSKREQEVTELKKTLEEETRIHEAAVQELRQRHG QALGELAEQLEQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGE RARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAME AEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKT ETVDRLERGRRRLQQELDDATMDLEQQRQLVSTLEKKQRKFDQLLAEEKAAVLRAVEERERAEAEGRERE ARALSLTRALEEEQEAREELERQNRALRAELEALLSSKDDVGKSVHELERACRVAEQAANDLRAQVTELE DELTAAEDAKLRLEVTVQALKTQHERDLQGRDEAGEERRRQLAKQLRDAEVERDEERKQRTLAVAARKKL EGELEELKAQMASAGQGKEEAVKQLRKMQAQMKELWREVEETRTSREEIFSQNRESEKRLKGLEAEVLRL QEELAASDRARRQAQQDRDEMADEVANGNLSKAAILEEKRQLEGRLGQLEEELEEEQSNSELLNDRYRKL LLQVESLTTELSAERSFSAKAESGRQQLERQIQELRGRLGEEDAGARARHKMTIAALESKLAQAEEQLEQ ETRERILSGKLVRRAEKRLKEVVLQVEEERRVADQLRDQLEKGNLRVKQLKRQLEEAEEEASRAQAGRRR LQRELEDVTESAESMNREVTTLRNRLRRGPLTFTTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGS PPAHPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145809 |
| ORF Size | 6108 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145809.2 |
| RefSeq Size | 6930 bp |
| RefSeq ORF | 6111 bp |
| Locus ID | 79784 |
| UniProt ID | Q7Z406 |
| Protein Pathways | Regulation of actin cytoskeleton, Tight junction, Viral myocarditis |
| Gene Summary | This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
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