MEMO1 (NM_001137602) Human Tagged ORF Clone
CAT#: RG227278
- TrueORF®
MEMO1 (tGFP-tagged) - Human mediator of cell motility 1 (MEMO1), transcript variant 2
ORF Plasmid: DDK
"NM_001137602" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C2orf4; CGI-27; MEMO; NS5ATP7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227278 representing NM_001137602
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGAGTGGTCTGCCGAGAAGCCAGTCACGCCGGGAGCTGGTACACAGCCTCAGGACCGCAGC TGAATGCACAGCTAGAAGGTTGGCTTTCACAAGTACAGTCTACAAAAAGACCTGCTAGAGCCATTATTGC CCCCCGGAGAATTTTCATCCTTGGGCCTTCTCATCATGTGCCCCTCTCTCGATGTGCACTTTCCAGTGTG GATATATATAGGACACCTCTGTATGACCTTCGTATTGACCAAAAGATTTACGGAGAACTGTGGAAGACAG GAATGTTTGAACGCATGTCTCTGCAGACAGATGAAGATGAACACAGTATTGAAATGCATTTGCCTTATAC AGCTAAAGCCATGGAAAGCCATAAGGATGAGTTTACCATTATTCCTGTACTGGTTGGAGCTCTGAGTGAG TCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATATCTAGCGGATCCTAGTAATCTCTTTGTGGTTT CTTCTGATTTCTGCCATTGGGGTCAAAGGTTCCGTTACAGTTACTATGATGAATCCCAGGGGGAGATTTA TAGATCCATTGAACATCTAGATAAAATGGGTATGAGTATTATAGAACAATTAGACCCTGTATCTTTTAGC AATTACTTGAAGAAATACCATAATACTATATGTGGAAGACATCCCATTGGGGTGTTATTAAATGCTATCA CAGAGCTCCAGAAGAATGGAATGAATATGAGTTTTTCGTTTTTGAATTATGCCCAGTCGAGCCAGTGTAG AAACTGGCAAGACAGTTCAGTGAGTTATGCAGCTGGAGCACTCACGGTCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227278 representing NM_001137602
Red=Cloning site Green=Tags(s) MSNRVVCREASHAGSWYTASGPQLNAQLEGWLSQVQSTKRPARAIIAPRRIFILGPSHHVPLSRCALSSV DIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSE SKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFS NYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQSSQCRNWQDSSVSYAAGALTVH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001137602 |
ORF Size | 822 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001137602.3 |
RefSeq Size | 1762 bp |
RefSeq ORF | 825 bp |
Locus ID | 51072 |
UniProt ID | Q9Y316 |
Gene Summary | May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227278 | MEMO1 (Myc-DDK-tagged)-Human mediator of cell motility 1 (MEMO1), transcript variant 2 |
CNY 2,400.00 |
|
RC227278L3 | Lenti ORF clone of Human mediator of cell motility 1 (MEMO1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC227278L4 | Lenti ORF clone of Human mediator of cell motility 1 (MEMO1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC325696 | MEMO1 (untagged)-Human mediator of cell motility 1 (MEMO1), transcript variant 2 |
CNY 6,270.00 |