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SH3BP2 (NM_001145855) Human Tagged ORF Clone
CAT＃: RG227989
- TrueORF®
SH3BP2 (tGFP-tagged) - Human SH3-domain binding protein 2 (SH3BP2), transcript variant 4. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001145855" in other vectors (4)
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CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3BP-2; 3BP2; CRBM; CRPM; RES4-23 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227989 ORF sequence, codon optimized.
Due to the complexity of NM_001145855, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCTCGGCCCAAGAACGCCCGCCCCCTCTAGAAGTAGAGGCAGAAGGGCGATGTGCTGGGTGA GCACCATATCTTTTATGGCTGCTGAGGAGATGCATTGGCCCGTACCAATGAAAGCTATTGGTGCCCAGAA TCTGCTTACTATGCCCGGTGGAGTGGCCAAAGCCGGCTATCTGCACAAGAAGGGCGGCACCCAGCTGCAA CTTCTTAAATGGCCACTTAGATTCGTGATTATCCACAAGCGGTGTGTGTACTACTTTAAAAGCAGCACCA GCGCCTCCCCTCAGGGAGCCTTTAGTCTGTCCGGCTATAACAGGGTGATGAGAGCCGCCGAAGAAACAAC ATCTAACAATGTTTTCCCTTTTAAAATCATCCATATTTCCAAGAAGCACCGGACCTGGTTCTTCAGCGCA TCCAGCGAGGAAGAAAGAAAAAGTTGGATGGCCCTGCTGCGGAGAGAAATAGGCCACTTTCACGAAAAAA AAGACCTGCCCCTGGATACCAGTGACTCATCCTCCGATACCGATAGCTTCTACGGGGCTGTCGAACGCCC CGTTGATATCTCCCTCAGCCCATACCCCACAGACAACGAGGATTACGAGCACGACGACGAAGACGACAGC TACCTGGAACCCGACAGCCCCGAACCGGGGAGACTCGAAGACGCTCTTATGCACCCTCCCGCTTACCCCC CACCCCCGGTGCCAACACCCCGCAAGCCCGCATTTAGCGATATGCCTCGGGCTCACAGTTTTACCTCAAA GGGGCCAGGCCCCCTTCTGCCACCTCCACCGCCAAAGCACGGCCTGCCAGACGTGGGCCTCGCGGCAGAG GACTCCAAACGAGATCCTCTGTGCCCTCGAAGGGCTGAGCCTTGTCCTAGGGTTCCAGCAACACCCCGCC GGATGTCCGACCCTCCCCTTTCCACAATGCCCACTGCTCCAGGACTGAGAAAGCCCCCATGCTTTAGGGA GTCTGCTAGCCCATCCCCGGAGCCTTGGACTCCAGGACACGGCGCGTGTAGCACATCAAGTGCCGCCATC ATGGCAACTGCAACTTCAAGGAACTGTGACAAGCTGAAATCCTTCCACCTCTCACCCCGCGGCCCCCCTA CCAGTGAGCCCCCACCAGTCCCGGCGAATAAGCCTAAGTTCTTGAAAATTGCCGAAGAAGATCCCCCACG CGAGGCGGCTATGCCTGGGCTCTTCGTGCCACCCGTTGCCCCCAGACCACCTGCACTGAAACTGCCTGTG CCCGAGGCAATGGCCAGACCCGCAGTCTTGCCTAGGCCCGAGAAACCTCAACTTCCACACCTGCAGAGAA GTCCTCCCGACGGCCAGAGCTTTAGAAGCTTCTCCTTCGAAAAACCGCGCCAACCTAGCCAGGCTGACAC AGGTGGCGACGATAGTGATGAGGATTACGAGAAGGTACCACTGCCTAACTCCGTCTTCGTGAATACCACG GAGAGCTGTGAAGTGGAACGCCTTTTCAAGGCAACTTCACCCAGAGGCGAACCCCAAGATGGGCTTTATT GTATAAGGAATTCATCAACTAAGTCCGGGAAAGTCCTGGTGGTTTGGGACGAGACATCCAACAAGGTGAG AAATTATAGGATCTTTGAGAAAGATTCAAAGTTTTATCTGGAAGGCGAAGTGCTTTTTGTGAGCGTTGGG TCTATGGTGGAACATTACCATACACATGTGCTCCCGTCTCATCAGTCCCTGCTTCTGAGGCACCCCTATG GCTACACAGGTCCAAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227989 representing NM_001145855
Red=Cloning site Green=Tags(s) MASLGPRTPAPSRSRGRRAMCWVSTISFMAAEEMHWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQ LLKWPLRFVIIHKRCVYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSA SSEEERKSWMALLRREIGHFHEKKDLPLDTSDSSSDTDSFYGAVERPVDISLSPYPTDNEDYEHDDEDDS YLEPDSPEPGRLEDALMHPPAYPPPPVPTPRKPAFSDMPRAHSFTSKGPGPLLPPPPPKHGLPDVGLAAE DSKRDPLCPRRAEPCPRVPATPRRMSDPPLSTMPTAPGLRKPPCFRESASPSPEPWTPGHGACSTSSAAI MATATSRNCDKLKSFHLSPRGPPTSEPPPVPANKPKFLKIAEEDPPREAAMPGLFVPPVAPRPPALKLPV PEAMARPAVLPRPEKPQLPHLQRSPPDGQSFRSFSFEKPRQPSQADTGGDDSDEDYEKVPLPNSVFVNTT ESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVG SMVEHYHTHVLPSHQSLLLRHPYGYTGPR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145855 |
| ORF Size | 1767 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145855.1, NP_001139327.1 |
| RefSeq Size | 9139 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 6452 |
| UniProt ID | P78314 |
| Protein Families | Druggable Genome |
| Protein Pathways | Natural killer cell mediated cytotoxicity |
| Gene Summary | The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227989 | SH3BP2 (Myc-DDK-tagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 4. Note: ORF is codon optimized |
CNY 4,384.00 |
|
| RC227989L3 | Lenti-ORF clone of SH3BP2 (Myc-DDK-tagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 4. Note: ORF is codon optimized |
CNY 7,130.00 |
|
| RC227989L4 | Lenti-ORF clone of SH3BP2 (mGFP-tagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 4. Note: ORF is codon optimized |
CNY 7,130.00 |
|
| SC326596 | SH3BP2 (untagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 4, mRNA |
CNY 9,600.00 |
