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SLI (SHC2) (NM_012435) Human Tagged ORF Clone
CAT＃: RG229293
- TrueORF®
SHC2 (tGFP-tagged) - Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2)
ORF Plasmid: DDK
"NM_012435" in other vectors (5)
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CNY 5,130.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SCK; SHCB; SLI |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229293 representing NM_012435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCAGGGTCCGGGCGGGCGCGCGCCCCCGGCGCCCCCCGCGCCCCCCGAGCCCGAGGCGCCCACCA CCTTCTGCGCGTTGCTGCCCCGAATGCCGCAGTGGAAGTTCGCGGCCCCGGGCGGCTTCCTGGGCCGCGG CCCGGCGGCGGCGCGGGCGGCTGGGGCCTCGGGGGGCGCGGACCCCCAACCCGAGCCGGCGGGCCCGGGG GGCGTCCCGGCGCTGGCGGCCGCCGTCCTGGGCGCCTGCGAGCCCCGCTGCGCCGCGCCCTGTCCCCTGC CCGCGCTCAGCCGCTGTCGGGGCGCGGGGTCGCGGGGGTCGCGGGGCGGGCGGGGGGCGGCGGGGTCCGG GGACGCCGCCGCCGCCGCCGAGTGGATCCGGAAGGGCAGCTTCATCCACAAACCCGCGCACGGCTGGCTA CACCCCGACGCCAGGGTCCTGGGGCCCGGGGTCTCCTACGTCGTGCGGTACATGGGCTGCATCGAGGTTC TCCGCTCTATGCGCTCCCTGGACTTTAACACGCGCACGCAGGTGACCAGGGAAGCCATCAACCGGCTCCA TGAGGCCGTGCCTGGCGTCCGGGGATCCTGGAAGAAAAAGGCCCCCAACAAGGCCCTGGCGTCCGTCCTG GGCAAGAGCAACCTTCGCTTTGCCGGCATGAGCATCTCCATCCACATCTCCACTGATGGCCTCAGCCTCT CCGTGCCTGCCACGCGCCAGGTCATCGCCAACCACCACATGCCGTCCATCTCCTTCGCGTCAGGCGGAGA CACGGACATGACGGATTACGTGGCCTACGTCGCCAAGGACCCCATCAACCAGAGAGCCTGCCACATCCTG GAGTGCTGTGAGGGCCTGGCACAGAGCATCATCAGCACCGTGGGCCAAGCTTTCGAGCTGCGCTTCAAGC AGTACCTGCACAGCCCGCCCAAGGTGGCGCTGCCCCCAGAAAGGCTGGCAGGGCCGGAGGAGTCGGCCTG GGGGGACGAGGAGGACTCTTTGGAGCACAATTACTACAACAGCATCCCGGGGAAGGAGCCGCCGCTGGGC GGGCTAGTGGACTCCAGGCTGGCCCTGACACAGCCCTGCGCCCTCACGGCCCTCGACCAGGGCCCATCTC CTTCTCTAAGAGATGCCTGCAGCCTGCCATGGGACGTGGGGTCCACCGGTACAGCTCCACCGGGGGACGG CTACGTGCAGGCGGACGCCCGGGGCCCCCCGGACCACGAGGAGCACCTGTATGTCAACACCCAGGGTCTG GACGCCCCCGAGCCGGAGGACAGCCCCAAAAAGGATCTGTTTGACATGCGACCCTTTGAGGATGCCCTGA AGTTGCATGAGTGCTCAGTGGCGGCAGGCGTGACAGCAGCCCCTCTTCCCTTGGAGGACCAGTGGCCCAG CCCCCCTACCCGCCGGGCCCCTGTGGCCCCCACGGAGGAACAGCTGCGTCAGGAGCCCTGGTACCACGGC CGGATGAGCCGCCGGGCGGCAGAGAGGATGCTTCGAGCTGACGGGGACTTCCTTGTGCGAGACAGCGTCA CCAACCCCGGGCAGTATGTCCTCACCGGCATGCACGCCGGGCAGCCCAAGCACCTGCTGCTCGTGGACCC CGAGGGCGTGGTACGGACGAAGGACGTGCTGTTTGAGAGCATCAGCCACCTGATCGACCACCACCTGCAG AACGGGCAGCCCATCGTGGCCGCCGAGAGTGAGCTGCACCTGCGTGGCGTGGTCTCACGGGAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229293 representing NM_012435
Red=Cloning site Green=Tags(s) MTQGPGGRAPPAPPAPPEPEAPTTFCALLPRMPQWKFAAPGGFLGRGPAAARAAGASGGADPQPEPAGPG GVPALAAAVLGACEPRCAAPCPLPALSRCRGAGSRGSRGGRGAAGSGDAAAAAEWIRKGSFIHKPAHGWL HPDARVLGPGVSYVVRYMGCIEVLRSMRSLDFNTRTQVTREAINRLHEAVPGVRGSWKKKAPNKALASVL GKSNLRFAGMSISIHISTDGLSLSVPATRQVIANHHMPSISFASGGDTDMTDYVAYVAKDPINQRACHIL ECCEGLAQSIISTVGQAFELRFKQYLHSPPKVALPPERLAGPEESAWGDEEDSLEHNYYNSIPGKEPPLG GLVDSRLALTQPCALTALDQGPSPSLRDACSLPWDVGSTGTAPPGDGYVQADARGPPDHEEHLYVNTQGL DAPEPEDSPKKDLFDMRPFEDALKLHECSVAAGVTAAPLPLEDQWPSPPTRRAPVAPTEEQLRQEPWYHG RMSRRAAERMLRADGDFLVRDSVTNPGQYVLTGMHAGQPKHLLLVDPEGVVRTKDVLFESISHLIDHHLQ NGQPIVAAESELHLRGVVSREP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012435 |
| ORF Size | 1746 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012435.2, NP_036567.2 |
| RefSeq Size | 2509 bp |
| RefSeq ORF | 1749 bp |
| Locus ID | 25759 |
| UniProt ID | P98077 |
| Protein Pathways | Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, VEGF signaling pathway |
| Gene Summary | Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229293 | SHC2 (Myc-DDK-tagged)-Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2) |
CNY 6,496.00 |
|
| RC229293L3 | Lenti-ORF clone of SHC2 (Myc-DDK-tagged)-Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2) |
CNY 6,560.00 |
|
| RC229293L4 | Lenti-ORF clone of SHC2 (mGFP-tagged)-Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2) |
CNY 6,560.00 |
|
| SC315998 | SHC2 (untagged)-Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2) |
CNY 9,310.00 |
|
| SC327928 | SHC2 (untagged)-Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2) |
CNY 9,310.00 |
