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PHF8 (NM_001184896) Human Tagged ORF Clone
CAT＃: RG230633
- TrueORF®
PHF8 (tGFP-tagged) - Human PHD finger protein 8 (PHF8), transcript variant 1
ORF Plasmid: DDK
"NM_001184896" in other vectors (6)
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CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | JHDM1F; KDM7B; MRXSSD; ZNF422 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230633 representing NM_001184896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAGGAGCCGCGCTATCGTCCAGAGAGGACGCGTGCTGCCGCCTCCCGCCCCTCTTGACACGACGA ACCTGGCCGGCCGCAGAACGCTCCAGGGCCGAGCGAAGATGGCCTCGGTGCCGGTGTATTGCCTCTGCCG GCTGCCTTACGATGTGACCCGCTTCATGATCGAGTGTGACATGTGCCAGGACTGGTTTCATGGCAGTTGT GTTGGTGTTGAAGAGGAGAAGGCTGCTGACATTGACCTCTACCACTGCCCCAACTGTGAAGTCTTGCATG GGCCCTCCATTATGAAAAAACGCCGTGGATCTTCAAAGGGGCATGATACACACAAGGGGAAACCAGTGAA GACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTAGGACTTTTGACAGCTCAGATGAAGTGATTCTG AAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGAAGAAAATAGCTTCAGTGTGCCCATCCTGGTCC TGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCATCATTCACTGTGAGGGATGTTGAACACTATGT TGGTTCTGACAAAGAGATTGATGTGATTGATGTGACCCGCCAGGCTGACTGCAAGATGAAGCTTGGTGAT TTTGTGAAATACTATTACAGCGGGAAGAGGGAGAAAGTCCTCAATGTCATTAGTTTGGAATTCTCTGATA CCAGACTTTCTAACCTTGTGGAGACACCGAAGATTGTTCGAAAGCTGTCATGGGTCGAAAACTTGTGGCC AGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGTACTGCCTCATGAGTGTGCGAGATAGCTATACA GACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTACCATGTACTCAAGGGTGAAAAGATCTTCTACC TGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAGTGCTGGAGCAGTTCCTCTAATCAGAATGAGAT GTTCTTTGGGGACCAGGTGGACAAGTGCTACAAGTGTTCCGTGAAGCAAGGACAGACACTTTTCATTCCC ACAGGGTGGATCCATGCTGTGCTGACGCCTGTGGACTGCCTTGCCTTTGGAGGGAACTTCTTACACAGCC TTAACATCGAGATGCAGCTCAAAGCCTATGAGATTGAGAAGCGGCTGAGCACAGCAGACCTCTTCAGATT CCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGCACATCCTGGACATCTTTCGCGGTTTGCGAGAG AACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGGCAAAGCCTTGAACTTGGCCTTTAGAGCCTGGA CAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATCCCGGAGACAGTGCGAACCGTACAGCTCATTAA AGATCTGGCCAGGGAGATCCGCCTGGTGGAAGACATCTTCCAACAGAACGTTGGGAAGACGAGCAATATC TTTGGGCTGCAGAGGATCTTCCCAGCCGGCTCCATTCCCCTAACCAGGCCAGCCCATTCCACTTCAGTGT CCATGTCCAGGCTGTCACTGCCCTCCAAAAATGGTTCAAAGAAGAAAGGCCTGAAGCCCAAGGAACTCTT CAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCTTGGGGCCTGCTGGCCAGTTGAGCTATAATCTC ATGGACACATACAGTCATCAGGCACTGAAGACAGGCTCTTTCCAGAAAGCAAAGTTCAACATCACTGGTG CCTGCTTGAATGACTCAGATGACGACTCACCAGACTTGGACCTTGATGGAAATGAGAGCCCATTGGCCCT ATTGATGTCTAACGGCAGTACGAAAAGGGTGAAGAGTTTATCCAAATCTCGGCGAACCAAGATAGCAAAG AAGGTAGACAAGGCTAGGCTGATGGCAGAACAGGTGATGGAAGACGAATTTGACTTGGATTCAGATGATG AGCTGCAGATTGACGAGAGATTGGGAAAGGAGAAGGCGACCCTGATAATAAGACCAAAATTTCCCCGGAA ATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAGTTCGTGAACCAGGAGAAGTTGAGTTTGACATT GAGGAGGACTATACAACAGATGAGGACATGGTGGAAGGGGTTGAAGGCAAGCTTGGGAATGGTAGTGGCG CTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAGGTGGGGGGACCTGACTATGCTGCCCTCACCGA GGCCCCAGCTTCTCCCAGCACTCAGGAGGCCATCCAGGGCATGCTGTGCATGGCCAACCTGCAGTCCTCA TCGTCCTCACCGGCTACCTCTAGCCTGCAGGCCTGGTGGACTGGGGGACAGGATCGAAGCAGTGGGAGCT CCAGCAGTGGGCTGGGCACAGTGTCTAACAGTCCTGCTTCCCAGCGCACCCCAGGGAAGCGGCCCATCAA GCGGCCAGCATACTGGAGAACCGAGAGCGAGGAGGAGGAGGAGAACGCCAGTCTGGATGAACAGGACAGC TTGGGAGCGTGCTTCAAGGATGCAGAGTATATCTATCCTTCACTGGAGTCTGATGATGATGACCCTGCTT TGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGATGCTCCATGGAGTCCTAAAGCCCGCGTGACCCC AACTCTGCCGAAGCAGGACCGTCCTGTGCGTGAGGGGACCCGGGTAGCCTCTATTGAGACAGGTTTGGCT GCAGCAGCTGCAAAGCTGGCCCAGCAGGAGCTACAGAAGGCCCAAAAGAAGAAATATATCAAGAAGAAGC CTTTGCTGAAGGAGGTAGAACAGCCTCGCCCTCAAGACTCCAATCTCAGTCTGACAGTACCAGCCCCCAC TGTGGCTGCCACACCACAACTTGTCACCTCCTCCTCACCCCTGCCTCCTCCTGAGCCTAAACAAGAGGCC CTGTCAGGAAGTCTCGCTGACCATGAGTACACCGCTCGTCCCAATGCCTTTGGCATGGCCCAGGCAAACC GCAGCACCACACCTATGGCCCCCGGTGTCTTCTTGACCCAGCGGCGCCCTTCAGTTGGCTCCCAGAGCAA TCAGGCAGGACAAGGAAAGCGTCCCAAAAAGGGCCTGGCCACAGCAAAGCAGAGACTCGGCCGTATCCTG AAAATCCACAGAAATGGCAAACTACTTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230633 representing NM_001184896
Red=Cloning site Green=Tags(s) MNRSRAIVQRGRVLPPPAPLDTTNLAGRRTLQGRAKMASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSC VGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVIL KPTGNQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGD FVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYT DFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVDKCYKCSVKQGQTLFIP TGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFRFPNFETICWYVGKHILDIFRGLRE NRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNI FGLQRIFPAGSIPLTRPAHSTSVSMSRLSLPSKNGSKKKGLKPKELFKKAERKGKESSALGPAGQLSYNL MDTYSHQALKTGSFQKAKFNITGACLNDSDDDSPDLDLDGNESPLALLMSNGSTKRVKSLSKSRRTKIAK KVDKARLMAEQVMEDEFDLDSDDELQIDERLGKEKATLIIRPKFPRKLPRAKPCSDPNRVREPGEVEFDI EEDYTTDEDMVEGVEGKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSS SSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDS LGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLA AAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNLSLTVPAPTVAATPQLVTSSSPLPPPEPKQEA LSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSNQAGQGKRPKKGLATAKQRLGRIL KIHRNGKLLL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001184896 |
| ORF Size | 3180 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184896.1, NP_001171825.1 |
| RefSeq Size | 5921 bp |
| RefSeq ORF | 3183 bp |
| Locus ID | 23133 |
| UniProt ID | Q9UPP1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230633 | PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 11,072.00 |
|
| RC230633L1 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| RC230633L2 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 13,472.00 |
|
| RC230633L3 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| RC230633L4 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| SC329271 | PHF8 (untagged)-Human PHD finger protein 8 (PHF8) transcript variant 1 |
CNY 20,430.00 |
