United States
Germany
Japan
United Kingdom
China
CNOT1 (NM_001265612) Human Tagged ORF Clone
CAT＃: RG235388
- TrueORF®
CNOT1 (tGFP-tagged) - Homo sapiens CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 3
ORF Plasmid: DDK
"NM_001265612" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 29,830.00
货期*
14周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AD-005; CDC39; HPE12; NOT1; NOT1H |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235388 representing NM_001265612
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA ATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT ATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG ACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCCTATG CCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAA AGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGAT CTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAG ACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCA TCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTTAAGACGCTG CGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACA TTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGA TTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTT GGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGAACTCATTCAGGAT TAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATAA AAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCA AGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGAC TTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCAGTAGACCTCATATATAG ACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAGAGATCTTCTGT TTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAG AAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGA GCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAA ATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGATGCCAATTTTCCTTGGAAACC ATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAACT TATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATA CTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTG ACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGATAAGTGGCTCACAGATAAAATTCGAGA GCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTATTTTGGGCGGA CTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCT GTCTGCAAGCTTGTGCAGGGAGTGTTTCTCAGGAGCTATCAGAAACTATCCTCACCATGGTAGCCAATTG CAGTAATGTTATGAATAAGGCCAGACAACCACCACCTGGAGTTATGCCAAAAGGACGTCCTCCTAGTGCT AGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGACATCTCTTAGTATAGGTG GTTCAGCTGCCCCTCACACCCAGAGTATGCAGGGTTTTCCTCCAAATTTGGGTTCTGCATTCAGTACCCC TCAGTCACCAGCAAAAGCATTTCCACCCCTTTCAACCCCCAATCAGACCACTGCATTCAGTGGTATTGGA GGACTTTCATCACAGCTTCCAGTAGGTGGTCTTGGCACAGGCAGCCTGACTGGTATAGGAACTGGTGCTC TTGGACTCCCTGCAGTGAATAACGACCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTGAATCAGCC TACATTCCAGCAGACGGACTTGTCTCAGGTGTGGCCAGAGGCAAACCAGCACTTTAGTAAAGAGATAGAT GATGAAGCAAACAGCTATTTCCAGCGAATATATAATCATCCACCACATCCAACCATGTCTGTTGATGAGG TATTAGAAATGCTGCAGAGATTTAAAGACTCTACTATAAAGAGGGAACGAGAAGTATTTAACTGTATGCT AAGGAACTTGTTTGAAGAATATCGTTTTTTTCCCCAGTATCCTGATAAAGAGTTACATATAACAGCCTGC CTATTTGGTGGTATAATTGAGAAAGGACTGGTCACTTACATGGCACTAGGTCTGGCTCTACGATATGTTC TTGAAGCCTTACGCAAGCCTTTTGGATCCAAAATGTATTATTTCGGGATTGCTGCACTAGATAGATTTAA AAACAGATTGAAGGACTATCCCCAGTATTGTCAGCATTTGGCTTCTATCAGTCACTTTATGCAATTTCCA CATCATTTACAGGAGTATATTGAGTATGGACAGCAGTCTAGAGATCCTCCTGTGAAAATGCAAGGCTCTA TCACAACCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCT TGCTGGTCAAGTTAGCACTATGGTAACCACCTCAACAACTACCACTGTTGCTAAAACGGTTACGGTCACC AGGCCAACTGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATTAATACTACAAATATAGATACGTTGC TTGTGGCCACAGATCAAACTGAGAGAATTGTGGAGCCCCCAGAAAATATCCAGGAGAAAATTGCTTTTAT TTTCAATAATCTCTCACAGTCAAATATGACACAAAAGGTTGAAGAGCTAAAGGAAACGGTGAAAGAAGAA TTTATGCCTTGGGTTTCACAGTATCTGGTTATGAAGAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGT ATTCAAACTTCCTTGACACGCTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAGACCTACAGAAA CATTAAAGTGCTCCTGACCTCTGATAAAGCTGCAGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTG GGACATTGGCTAGGAATGATCACATTAGCTAAAAACAAACCCATCTTACACACTGACTTGGATGTGAAAT CATTGCTGCTAGAGGCTTATGTTAAAGGACAACAAGAATTGCTCTATGTAGTGCCCTTTGTTGCCAAAGT CTTAGAATCTAGCATTCGTAGTGTGGTTTTTAGGCCACCAAACCCTTGGACAATGGCAATTATGAATGTA TTAGCTGAGCTACATCAGGAGCATGACTTAAAGTTAAACTTGAAGTTTGAAATCGAGGTTCTCTGCAAGA ACCTTGCATTAGACATCAATGAGCTAAAACCTGGAAACCTCCTAAAGGATAAAGATCGCCTGAAGAATTT AGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCAGAAGAACTCCCTCCCATCACAACCACA ACAACTTCTACTACACCAGCTACCAACACCACTTGTACAGCCACGGTTCCACCACAGCCACAGTACAGCT ACCACGACATCAATGTCTATTCCCTTGCGGGCTTGGCACCACACATTACTCTGAATCCAACAATTCCCTT GTTTCAGGCCCATCCACAGTTGAAGCAGTGTGTGCGTCAGGCAATTGAACGGGCTGTCCAGGAGCTGGTC CATCCTGTGGTGGATCGATCAATTAAGATTGCCATGACTACTTGTGAGCAAATAGTCAGGAAGGATTTTG CCCTGGATTCGGAGGAATCTCGAATGCGAATAGCAGCTCATCACATGATGCGTAACTTGACAGCTGGAAT GGCTATGATTACATGCAGGGAACCTTTGCTCATGAGCATATCTACCAACTTAAAAAACAGTTTTGCCTCA GCCCTTCGTACTGCTTCCCCACAACAAAGAGAAATGATGGATCAGGCAGCTGCTCAATTAGCTCAGGACA ATTGTGAGTTGGCTTGCTGTTTTATTCAGAAGACTGCAGTAGAAAAAGCAGGCCCTGAGATGGACAAGAG ATTAGCAACTGAATTTGAGCTGAGAAAACATGCTAGGCAAGAAGGACGCAGATACTGTGATCCTGTTGTT TTAACATATCAAGCTGAACGGATGCCAGAGCAAATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGT TGGCTGTTTACGAAGAGTTTGCACGCAATGTTCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCCAC GGGATTTTTAGCCCAGCCCATGAAGCAAGCTTGGGCAACAGATGATGTAGCTCAGATTTATGATAAGTGT ATTACAGAACTGGAGCAACATCTACATGCCATCCCACCAACTTTGGCCATGAACCCTCAAGCTCAGGCTC TTCGAAGTCTCTTGGAGGTTGTAGTTTTATCTCGAAACTCTCGGGATGCCATAGCTGCTCTTGGATTGCT CCAAAAGGCTGTAGAGGGCTTACTAGATGCCACAAGTGGTGCTGATGCTGACCTTCTGCTGCGCTACAGG GAATGCCACCTCTTGGTCCTAAAAGCTCTGCAGGATGGCCGGGCATATGGGTCTCCATGGTGCAACAAAC AGATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAGCTGCTAAT TCGCAATCATTTGGTTAATATGCAGCAGTATGATCTTCACCTAGCGCAGTCAATGGAGAATGGCTTAAAC TACATGGCTGTGGCATTTGCTATGCAGTTAGTAAAAATCCTGCTGGTGGATGAAAGGAGTGTTGCTCATG TTACTGAGGCAGATCTGTTCCACACCATTGAAACCCTCATGAGGATTAATGCTCATTCCAGAGGCAATGC TCCAGAAGGATTGCCCCAGCTGATGGAAGTAGTGCGATCCAACTATGAAGCAATGATTGATCGTGCTCAT GGAGGCCCAAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAGTATGATGACCCTCCAGGCCTGA GGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAG TACCAAAGCTTTCTCTGCATTTGTTGGACAGATGCACCAGCAAGGAATACTGAAGACCGATGATCTCATA ACAAGGTTCTTTCGTCTGTGTACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCTGAGCAGCAGC ACAATCCTGCTGCCAATCCCACCATGATCCGAGCCAAGTGCTATCACAACCTGGATGCCTTTGTTCGACT CATTGCACTGCTCGTGAAACACTCAGGGGAGGCCACCAACACTGTCACAAAGATTAATCTGCTGAACAAG GTCCTTGGTATAGTAGTGGGAGTTCTCCTTCAGGATCATGATGTTCGTCAGAGTGAATTTCAGCAACTTC CCTACCATCGAATTTTTATCATGCTTCTCTTGGAACTCAATGCACCTGAGCATGTGTTGGAAACCATTAA TTTCCAGACACTTACAGCTTTCTGCAATACATTCCACATCTTGAGGCCTACCAAAGCTCCTGGCTTTGTA TATGCCTGGCTTGAACTGATTTCCCATCGGATATTTATTGCAAGAATGCTGGCACATACGCCACAGCAGA AGGGGTGGCCTATGTATGCACAGCTACTGATTGATTTATTCAAATATTTAGCGCCTTTCCTTAGAAATGT GGAACTCACCAAACCTATGCAAATCCTCTACAAGGGCACTTTAAGAGTGCTGCTGGTTCTTTTGCATGAT TTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCACCTAATTGTATCCAGTTAA GAAATTTGATCCTGAGTGCCTTTCCAAGAAACATGAGGCTCCCCGACCCATTCACTCCTAATCTAAAGGT GGACATGTTGAGTGAAATTAACATTGCTCCCCGGATTCTCACCAATTTCACTGGAGTAATGCCACCTCAG TTCAAAAAGGATTTGGATTCCTATCTTAAAACTCGATCACCAGTCACTTTCCTGTCTGATCTGCGCAGCA ACCTACAGGTATCCAATGAACCTGGGAATCGCTACAACCTCCAGCTCATCAATGCACTGGTGCTCTATGT CGGGACTCAGGCCATTGCGCACATCCACAACAAGGGCAGCACACCTTCAATGAGCACCATCACTCACTCA GCACACATGGATATCTTCCAGAATTTGGCTGTGGACTTGGACACTGAGGGTCGCTATCTCTTTTTGAATG CAATTGCAAATCAGCTCCGGTACCCAAATAGCCACACTCACTACTTCAGTTGCACCATGCTGTACCTTTT TGCAGAGGCCAATACGGAAGCCATCCAAGAACAGATCACAAGAGTTCTCTTGGAACGGTTGATTGTAAAT AGGCCACATCCTTGGGGTCTTCTTATTACCTTCATTGAGCTGATTAAAAACCCAGCGTTTAAGTTCTGGA ACCATGAATTTGTACACTGTGCCCCAGAAATCGAAAAGTTATTCCAGTCGGTCGCACAGTGCTGCATGGG ACAGAAGCAGGCCCAGCAAGTAATGGAAGGGACAGGTGCCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235388 representing NM_001265612
Red=Cloning site Green=Tags(s) MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ TQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSD LRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTL RRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQF GVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNP SLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFC FADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQ INTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRI LDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGG LAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSA SSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIG GLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEID DEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITAC LFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFP HHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVT RPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEE FMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNL GHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNV LAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTT TTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELV HPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFAS ALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVV LTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKC ITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYR ECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLN YMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAH GGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLI TRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNK VLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFV YAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHD FPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQ FKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHS AHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVN RPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001265612 |
| ORF Size | 7113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001265612.2 |
| RefSeq Size | 8461 bp |
| RefSeq ORF | 7116 bp |
| Locus ID | 23019 |
| UniProt ID | A5YKK6 |
| Protein Pathways | RNA degradation |
| Gene Summary | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
You may also need
Customer
Reviews
Loading...
