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ULK3 (NM_001284364) Human Tagged ORF Clone
CAT＃: RG238421
- TrueORF®
ULK3 (tGFP-tagged) - Human unc-51 like kinase 3 (ULK3), transcript variant 2
ORF Plasmid: DDK
"NM_001284364" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG238421 representing NM_001284364.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTGGGCAGCGGC ACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTGGTAGCCATAAAGTGTGTA GCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTCACGGAGATTGAGATCCTCAAGGGCATT CGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGGGACAGTGACAATATCTACCTCATCATGGAG TTTTGCGCAGGGGGCGACCTGTCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGT GTCTTCATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTG AAGCCACAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCA CAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATG GTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGAAGCCCTC TTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAGCTGGAAGAGAAGATCCGTAGCAACCGGGTC ATCGAGCTCCCCTTGCGGCCCCTGCTCTCCCGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGG GACCCCAGCCGTCGCATCTCCTTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCC AGTGGGGAGAGTCTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGAT TCAGCAGCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTATGAAGTG GATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGGGCTGAGGAGCTCAAG GCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACCTCTGCCCGAGACCTGCTCAGAGAG ATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAG GCCGCCGGCGGGGAGCAGGATGCCCTGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTG GCAGCGGAGCCCCCGGGCCGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCT GAATACTTGAAGGAGCAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGACTGTCG GAATCTGTTCGTAGCTCTTGCACCCTTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG238421
Blue=ORF Red=Cloning site Green=Tag(s) MAGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGI RHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDL KPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSMGVILYEAL FGQPPFASRSFSELEEKIRSNRVIELPLRPLLSRDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMP SGESLGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELK AIVSSSNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLL AAEPPGRRRELLHTEVQNLMARAEYLKEQMRESRWEADTLDKEGLSESVRSSCTLQ TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001284364 |
| ORF Size | 1410 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001284364.3 |
| RefSeq Size | 2638 bp |
| RefSeq ORF | 1413 bp |
| Locus ID | 25989 |
| UniProt ID | Q6PHR2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
| MW | 53.7 kDa |
| Gene Summary | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
