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TOP3B (NM_001282113) Human Tagged ORF Clone
CAT＃: RG239704
- TrueORF®
TOP3B (tGFP-tagged) - Human topoisomerase (DNA) III beta (TOP3B), transcript variant 3
ORF Plasmid: DDK
"NM_001282113" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | TOP3B1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239704 representing NM_001282113.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGA GGGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCT GGCCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAA TACAACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCT AACCCCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGG CTGGACTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAAC AAGGCCCATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGT AATGCCATGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTG GACCTGCGAATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTA GACAGCTCTCTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGAT AAAATCCAGTCCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGA TCTCTCCTTTTGGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATG ACAAAGCTGGAGAAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTG GCCCTGAACACTGTGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATG CAGACGGCTGAGCGGCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCT GAGAACTTTGACCTGAAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAG CGGTTGTTAGCAGAAGGTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACC CCCATGAAGTCTGCCACAGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGA CACTTCATCGCCACGGTCAGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCC GAGCTCTTCACCTGCTCCGGGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGC GTGCCCCTGGAGGAGAGCCTGCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATG CTGGAGAAGCAGACGAACCCACCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCAT GGCATCGGCACGGATGCCAGCATCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTG GAGAGCGGGCGCCGGCTCAAGCCCACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGAT GCAGAGCTGGTGCTCCCCACCATCCGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAG GCCGACTACCGCCAGGTCCTGGGCCACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGAC TCCATTGCTGGCATGGATGAGTTGATGGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTC TCACGCTGTGGGAAGTGCCACCGCTTCATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCC CACTGCGATGAGACCTACACGCTCCCCCAGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCT CTGGATGACTTCGAGCTGGTCCTGTGGTCATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTAC TGCTACAACCACCCACCCTTCCGAGACATGAAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCC TGCCAGCACTCGCTGAGCATGCTGGGCATCGGCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTG GACCCCACCTCGGGCCCCAAGTGGAAGGTGGCCTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAG AACGCCCACCGCGTGCGGGTGTCCGCCGACACCTGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGAC TTCAACAAGGCCAAGTCCCCACTCCCGGGCGATGAGACGCAGCACATGGGCTGCGTCTTTTGTGACCCC GTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGCCTCCTGCCACCCCATGCACCGCGGTGGACCAGGG AGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGGCCCCCTGGGAAGCCCAACCCCAGACGGCCCAAG GACAAGATGTCAGCCCTGGCCGCCTACTTTGTA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239704
Blue=ORF Red=Cloning site Green=Tag(s) MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGK YNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMN KAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDL DSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNM TKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYP ENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITR HFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKM LEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKID AELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPL SRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPY CYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFE NAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPG RRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282113 |
| ORF Size | 2586 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001282113.2 |
| RefSeq Size | 2901 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 8940 |
| UniProt ID | O95985 |
| Protein Pathways | Homologous recombination |
| MW | 96.7 kDa |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013] |
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