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HERC4 (NM_001278185) Human Tagged ORF Clone
CAT＃: RG239850
- TrueORF®
HERC4 (tGFP-tagged) - Human HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3
ORF Plasmid: DDK
"NM_001278185" in other vectors (2)
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CNY 7,510.00
Product images
Specifications
| Product Data | |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239850 representing NM_001278185.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTA GAGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACT GTGTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAA AAATCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGA GAAGCTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAG CTTGGCCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAG ATTGTACAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGG GGACAGAATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGCAGCTGCTT AAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTT TCTGGAGCTATCTTTGGATGGGGACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGG TATGTTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCAT ACTGCTGCTCTAACCAAGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCAT AATTCTACCAGTCATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATT GCTTGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGT GGTAATGGGCAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGG TACCCCTATAATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTC TCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGA TGTCCCAATCCGACAAAGCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCT TCTGGAAGGTTTCCTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAAT GGAAGTTTTTTAGCTGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATG AATGCTGCTAGGCTTTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCA GCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTT TATCTTACTCTACCAGAATGTCCCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTT GGTACAGCTCTTGTGAACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAA CCTCCACTATTCCTCAAGATAGTAGAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAG ATCGGTATTCCCCCTTCTGAAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAA ATACTACATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTA CAAGAATTGATAGACATAAGAAATGATTATATCAACTGGGTCCAACAGCAGGCCTATGGAATGGATGTC AACCATGGATTAACTGAGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCC CAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCCAC AGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGCTTAATTCTAGTGGTG CGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAG CCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTGCGCAAAGAATTTTTCTTG CTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCATT TGGTTTTCTGATAAGATCACAGTTGAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGT GCAGACACAGCTGTTAACAAACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAAT AAATCAGTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTT CTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGATTGGAAGGAACTG GAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGAACATCCTACGATAAAAATTTTTTGGGAAGTA TTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTGTTATTTTTGACAGGTAGTGATCGCATTCCT ATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTT TCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTG ATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239850
Blue=ORF Red=Cloning site Green=Tag(s) MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHE KSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQ IVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTL SGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGH NSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNW YPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYP SGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVA ASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLE PPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEV QELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAH RQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFL LIMRELLDPKYGMFRYYEDSRLIWFSDKITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFN KSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEV FHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKL IQAIDHNEGFSLI TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001278185 |
| ORF Size | 2937 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001278185.2 |
| RefSeq Size | 4297 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 26091 |
| UniProt ID | Q5GLZ8 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 110 kDa |
| Gene Summary | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC239850 | HERC4 (myc-DDK-tagged) - Human HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3 |
CNY 7,130.00 |
|
| SC337744 | HERC4 (untagged) - Human HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3 |
CNY 9,500.00 |
