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Tpm1 (NM_001034073) Rat Untagged Clone
CAT＃: RN200472
Tpm1 (untagged ORF) - Rat tropomyosin 1, alpha (Tpm1), transcript variant 7, (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Alpha-tm; Tma2; Tmsa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200472 representing NM_001034073
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTAGCAGCTCGCTGGAGGCGGTGCGGAGAAAGATCCGGAGCCTGCAGGAACAGGCAGATGCCG CGGAGGAGCGTGCGGGCAGCCTGCAGCGAGAGCTGGACCAGGAGCGGAAGCTGCGGGAAACCGCTGAAGC TGACGTAGCATCTCTGAACAGACGCATCCAGCTGGTTGAGGAGGAGTTGGATCGCGCTCAGGAGCGTCTG GCCACAGCTCTACAGAAGCTGGAGGAGGCTGAGAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCA TTGAAAGCCGAGCCCAAAAAGATGAAGAAAAGATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCA CATTGCTGAAGATGCTGACCGAAAGTATGAAGAGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGATCTG GAGCGTGCGGAGGAGAGGGCTGAGCTCTCGGAAGGCAAATGTGCCGAGCTTGAAGAAGAGTTGAAAACGG TGACGAACAACTTGAAGTCACTGGAGGCTCAGGCTGAGAAGTACTCTCAGAAAGAAGACAAGTATGAAGA GGAGATCAAGGTTCTCTCTGACAAGCTGAAGGAGGCTGAGACCCGGGCTGAGTTTGCAGAGAGATCAGTA ACCAAATTGGAGAAAAGCATTGATGACTTAGAAGAGAAAGTGGCACATGCCAAAGAAGAAAACCTTAGTA TGCACCAGATGCTGGACCAGACTTTACTGGAGCTAAACAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001034073 |
| Insert Size | 747 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001034073.1, NP_001029245.1 |
| RefSeq Size | 1677 bp |
| RefSeq ORF | 747 bp |
| Locus ID | 24851 |
| UniProt ID | P04692 |
| Gene Summary | This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (Tpm1.8, also known as variant 7) has multiple differences in the coding region compared to variant 1. These differences result in an isoform (Tpm1.8cy, also referred to as TM-5a) that is shorter and has distinct N- and C-termini compared to isoform a. Isoform Tpm1.8cy is the same length as isoforms Tpm1.9cy and Tpm1.13 but is the product of alternate splicing and contains short regions of sequence differences. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200472 | Tpm1 (Myc-DDK-tagged ORF) - Rat tropomyosin 1, alpha (Tpm1), transcript variant 7, (10 ug) |
CNY 3,990.00 |
|
| RR200472L3 | Lenti ORF clone of Tpm1 (Myc-DDK-tagged ORF) - Rat tropomyosin 1, alpha (Tpm1), transcript variant 7, (10 ug) |
CNY 6,080.00 |
|
| RR200472L4 | Lenti ORF clone of Tpm1 (mGFP-tagged ORF) - Rat tropomyosin 1, alpha (Tpm1), transcript variant 7, (10 ug) |
CNY 6,650.00 |
