Tpm1 (NM_001301336) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Alpha-tm; Tma2; Tmsa
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216243 representing NM_001301336 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAAGAGAACGCCTTGGATCGAGCAGAGC AGGCGGAGGCTGACAAGAAGGCTGCGGAAGACCGGAGCAAGCAGCTGGAAGATGAGCTGGTGTCACTGCA AAAGAAACTCAAGGGCACTGAAGATGAACTGGACAAATACTCCGAGGCTCTCAAAGATGCCCAGGAGAAA CTGGAGCTGGCGGAGAAAAAGGCCACAGATGCTGAAGCTGACGTAGCATCTCTGAACAGACGCATCCAGC TGGTTGAGGAGGAGTTGGATCGCGCTCAGGAGCGTCTGGCCACAGCTCTACAGAAGCTGGAGGAGGCTGA GAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCATTGAAAGCCGAGCCCAAAAAGATGAAGAAAAG ATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCACATTGCTGAAGATGCTGACCGAAAGTATGAAG AGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGATCTGGAGCGTGCGGAGGAGAGGGCTGAGCTCTCGGA AGGCAAATGTGCCGAGCTTGAAGAAGAGTTGAAAACGGTGACGAACAACTTGAAGTCACTGGAGGCTCAG GCTGAGAAGTACTCTCAGAAAGAAGACAAGTATGAAGAGGAGATCAAGGTTCTCTCTGACAAGCTGAAGG AGGCTGAGACCCGGGCTGAGTTTGCAGAGAGATCAGTAACCAAATTGGAGAAAAGCATTGATGACTTAGA AGACGAGCTGTATGCTCAGAAACTGAAGTACAAGGCCATCAGCGAGGAGCTGGACCACGCTCTCAACGAT ATGACTTCCATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301336
Insert Size	855 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301336.1, NP_001288265.1
RefSeq Size	1235 bp
RefSeq ORF	855 bp
Locus ID	24851
UniProt ID	P04692
Gene Summary	This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (Tpm1.1st, also known as variant 10) contains a alternate 3' exon compared to variant 1. The resulting protein (isoform Tpm1.1st) is shorter and has a distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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