Twist1 (NM_053530) Rat Tagged ORF Clone
CAT#: RR200631
- TrueORF®
Twist1 (Myc-DDK-tagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug)
"NM_053530" in other vectors (5)
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CNY 3,600.00
Cited in 13 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Twist |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200631 representing NM_053530
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAGGACGTGTCCAGCTCGCCAGTCTCGCCGGCCGACGACAGCCTGAGCAACAGCGAGGAGGAGC CGG[AT]CCGGCAGCAGCCGGCGAGCGGCAAGCGGGGGGCTCGCAAGAGACGCAGCAGTCGGCGCAGCGC GGGCGGCAGCGCGGGGCCCGGCGGGGCCACGGGCGGGGGCATCGGAGGCGGCGACGAGCCGGGCAGCCCG GCCCAGGGCAAGCGCGGCAAGAAATCTGCGGGCGGAGGCGGCGGCGCGGGCGGAGGCGGCGGCGGCGGCG GCAGCAGCAGCGGGGGCGGGAGCCCGCAGTCGTACGAGGAGCTGCAGACACAGCGGGTCATGGCCAACGT GCGGGAGCGCCAGCGCACCCAGTCGCTGAACGAGGCATTTGCCGCCCTGCGCAAGATCATCCCCACGCTG CCCTCGGACAAGCTGAGCAAGATTCAGACCCTCAAACTGGCGGCCAGGTACATCGACTTCCTGTACCAGG TCCTGCAGAGCGACGAGCTGGACTCCAAGATGGCAAGCTGCAGCTATGTGGCCCACGAGCGGCTCAGCTA CGCCTTCTCCGTCTGGAGGATGGAGGGGGCCTGGTCCATGTCCGCGTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200631 representing NM_053530
Red=Cloning site Green=Tags(s) MMQDVSSSPVSPADDSLSNSEEEPXXRQQPASGKRGARKRRSSRRSAGGSAGPGGATGGGIGGGDEPGSP AQGKRGKKSAGGGGGAGGGGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTL PSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_053530 |
ORF Size | 609 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053530.2, NP_445982.1 |
RefSeq Size | 1513 bp |
RefSeq ORF | 612 bp |
Locus ID | 85489 |
MW | 21 kDa |
Gene Summary | beta helix loop helix transcription factor [RGD, Feb 2006] |
Citations (13)
The use of this cDNA Clones has been cited in the following citations: |
---|
INT6/eIF3e represses E-cadherin expression through HIF2α in lung carcinoma A549 cells.
,null,
Genes to cells : devoted to molecular & cellular mechanisms
,PubMed ID 36116043
[Twist1]
|
TWIST1 -Reprogrammed Endothelial Cell Transplantation Potentiates Neovascularization-Mediated Diabetic Wound Tissue Regeneration.
,null,
Diabetes
,PubMed ID 32234721
[Twist1]
|
α-Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling.
,null,
Oncogene
,PubMed ID 30804457
[Twist1]
|
TWIST1-WDR5- Hottip regulates Hoxa9 chromatin to facilitate prostate cancer metastasis
,null,
Cancer research
,PubMed ID 28484075
[Twist1]
|
ETS2 and Twist1 promote invasiveness of Helicobacter pylori -infected gastric cancer cells by inducing Siah2
,null,
Biochemical Journal
,PubMed ID 27048589
[Twist1]
|
Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes
,null,
PLoS ONE
,PubMed ID 26361389
[Twist1]
|
Twist1 correlates with poor differentiation and progression in gastric adenocarcinoma via elevation of FGFR2 expression
,null,
World Journal of Gastroenterology : WJG
,PubMed ID 25561797
[Twist1]
|
MicroRNA-106b modulates epithelial-mesenchymal transition by targeting TWIST1 in invasive endometrial cancer cell lines.
,null,
Molecular carcinogenesis
,PubMed ID 24002805
[Twist1]
|
The emerging role of Twist proteins in hematopoietic cells and hematological malignancies
,null,
Blood Cancer Journal
,PubMed ID 24769647
[Twist1]
|
Presence of Twist1-positive neoplastic cells in the stroma of chromosome-unstable colorectal tumors.
,null,
Gastroenterology
,PubMed ID 23684708
[Twist1]
|
Mutations of TCF12 , encoding a basic-helix-loop-helix partner of TWIST1, are a frequent cause of coronal craniosynostosis
,null,
Nature genetics
,PubMed ID 23354436
[Twist1]
|
Investigation of FGFR2-IIIC Signaling via FGF-2 Ligand for Advancing GCT Stromal Cell Differentiation
,null,
PLoS ONE
,PubMed ID 23071632
[Twist1]
|
Astrocytes Upregulate Survival Genes in Tumor Cells and Induce Protection from Chemotherapy
,null,
Neoplasia (New York, N.Y.)
,PubMed ID 21390191
[Twist1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200631 | Twist1 (untagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug) |
CNY 3,990.00 |
|
RR200631L1 | Lenti ORF clone of Twist1 (Myc-DDK-tagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug) |
CNY 6,080.00 |
|
RR200631L2 | Lenti ORF clone of Twist1 (mGFP-tagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug) |
CNY 6,000.00 |
|
RR200631L3 | Lenti ORF clone of Twist1 (Myc-DDK-tagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug) |
CNY 6,080.00 |
|
RR200631L4 | Lenti ORF clone of Twist1 (mGFP-tagged ORF) - Rat twist homolog 1 (Drosophila) (Twist1), (10 ug) |
CNY 6,650.00 |