Nup98 (NM_031074) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200873 representing NM_031074 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACAAATCATTTGGAACCCCCTTTGGGGGTGGTACAGGGGGCTTTGGTACAACGTCAACATTTG GACAAAATACTGGCTTTGGTACTACTAGTGGAGGAGCATTTGGAACATCTGCATTTGGTTCTAGCAATAA TACTGGAGGCTTATTTGGTAATTCACAGACCAAACCAGGAGGACTGTTTGGTACCAGTTCATTTAGCCAG CCAGCAACCTCCACAAGCACTGGTTTTGGGTTTGGCACATCAACAGGAACATCAAATAGTTTGTTTGGAA CTGCAAATACAGGGACCAGTCTTTTCTCATCCCAGAACAATGCATTTGCACAAAATAAACCAACTGGATT TGGGAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACTACAAATACCACCTCTAATCCTTTT GGTAACACATCTGGCTCCCTCTTTGGGCCAAGTAGTTTTACAGCAGCACCTACAGGAACTACCATCAAAT TTAATCCTCCCACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATCAGCACCAAGCATCA GTGTATTACTGCCATGAAAGAATATGAAAGCAAGTCATTAGAGGAGCTTCGTTTGGAGGACTATCAGGCT AACCGGAAAGGCCCACAGAACCAAGTGGGGGCAGGTACCACAACTGGCTTATTTGGGTCTTCTCCAGCGA CTTCCAGTGCAACAGGACTCTTCAGCTCTTCCACCACTAATTCAGCCTTTTCATATGGTCAGAACAAAAC TGCCTTTGGAACTAGCACAACTGGATTTGGGACAAATCCAGGTGGCCTCTTTGGCCAACAGAATCAACAG ACTACCAGTCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCCAAATACTGGCTTTTCCTTTGGTA ATACCAGCACCCTTGGACAACCAAGCACCAATACTATGGGCCTATTTGGAGTAACCCAAGCCTCACAGCC AGGAGGTCTTTTTGGGACCGCTACAAACACCAGCACTGGGACAGCATTTGGGACAGGAACAGGTCTCTTT GGGCAGCCCAATACTGGATTTGGTGCAGTTGGTTCGACGCTGTTTGGCAATAACAAGCTTACAACTTTTG GAACCAGCACAACCAGTGCTCCCTCATTTGGTACAACCAGTGGCGGGCTCTTCGGTAACAAACCAACCCT GACTTTAGGAACCAATACAAACACTTCCAATTTTGGTTTTGGCACAAATAACAGTGGGAGTAGTATTTTT GGAAGTAAGCCAGCAGCCGGGACTCTGGGAACAGGACTTGGTACAGGATTTGGAACAGCTCTTGGTGCTG GACAGGCATCTTTGTTTGGAAACAACCAACCTAAGATTGGAGGGCCTCTTGGTACAGGAGCCTTTGGGGC CCCTGGATTTAATACTTCGACTGCCATTTTGGGCTTTGGCGCCCCCCAGGCCCCAGTAGCTTTGACAGAT CCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACCTCAATAGTCTAACATACTCACCCTTTG GAGACTCCCCTCTCTTCCGGAATCCTATGTCAGATCCTAAGAAGAAAGAAGAGAGACTGAAACCAACCAA TCCAGCAGCTCAGAAAGCTCTTACAACCCCTACTCATTATAAACTTACACCTCGCCCTGCCACCAGAGTC AGACCAAAGGCTTTGCAAACAACAGGTACAGCCAAATCACATCTCTTTGATGGGCTGGATGACGATGAAC CATCTCTAGCCAACGGAGCGTTTATGCCTAAAAAGAGCATCAAGAAGTTGGTTTTGAAAAATCTTAACAA TAGCAATCTCTTTTCTCCTGTTAATCATGATTCAGAAGATCTAGCTTCACCCTCTGAGTATCCAGAAAAT GGAGAGAGATTCAGCTTCCTGAGCAAACCTGTTGATGAGAACCATCAGCAGGATGGAGATGATGACTCTC TTGTATCACGATTTTACACTAATCCTATTGCCAAACCTATTCCACAAACTCCAGAGAGTGCTGGAAACAA AAATAACAGTAGCAGCAATGTGGAAGACACTTTCATTGCTTTGAACATGCGTGCTGCTTTGCGAAATGGA CTGGAAGGAAGCAGTGAAGAAACGTCTTTCCATGATGAGTCATTGCAAGATGACCGAGACGAGATAGAAA ACAGTGCTTTCCAAATCCATCCAGCAGGCATTGTTCTCACAAAAGTTGGTTATTATACTATTCCATCTAT GGATGACCTTGCTAAAATTACCAATGAGAAAGGAGAATGCATTGTTTCTGACTTCACCATTGGCCGTAAA GGGTATGGCTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAATTTGGATGACATTGTGCATA TCCGGAGGAAAGAAGTTATTGTCTATGTAGATGATAACCAAAAGCCACCTGTGGGTGAAGGACTAAATAG GAAGGCTGAAGTTACTTTGGATGGAGTTTGGCCAACAGATAAAACATCCCGGTGTTTAATAAAGAGTCCA GATCGACTTGCTGATATCAATTATGAGGGGAGATTAGAAGCAGTCTCAAGAAAGCAAGGGGCCCAATTCA AGGAGTATCGGCCTGAAACTGGTTCTTGGGTGTTTAAGGTTTCCCATTTTTCTAAGTATGGCCTTCAGGA TTCTGATGAAGAGGAGGAGGAACACCCACCCAAAACTACTTCAAAGAAGCTGAAGACTGCCCCTTTGCCC CCTGCAGGCCAGGCAACCACTTTCCAGATGACTCTTAATGGCAAACCTGCACCCCCACCTCAGAGCCAGA GCCCAGAAGTGGAGCAGTTAGGGAGGGTTGTGGAGCTGGACAGCGACATGGTAGATATCACGCAGGAGCC AGTTCCGGATTCGGTGTTAGAAGAGAGTGTACCTGAGGATCAGGAGCCCGTGTCCGCATCAACACAGATC GCGTCTTCACTGGGAATTAATCCACATGTGTTACAGATCATGAAAGCATCCTTGCTTGTGGATGAAGAAG ACGTAGATGCCATGGAGCAGCGCTTCGGTCACTTCCCTTCCAGAGGAGACACTGCTCAGGAGATCTGCTC CCCTAGACTCCCCATTTCAGCCTCCCACTCCTCAAAATCCCGCTCCATAGTTGGTGGGTTGCTCCAATCA AAATTTGCAAGTGGAACTTTTCTTTCACCAAGTGCCTCCGTGCAAGAATGTCGCACACCCAGAACATCAT CTCTAATGAACGTCCCATCCACATCCCCCTGGTCTGTCCCTCTACCCCTGGCCACTGTGTTCACAGTGCC CAGCCCAGCCCCTGAAGTTCCCCTAAAAACAGTGGGGATACGCAGGCAACCAGGCCTAGTCCCTCTTGAA AAATCCATTACATATGGCAAGGGAAAACTCTTGATGGACATGGCCTTATTCATGGGACGTTCATTTCGGG TTGGCTGGGGTCCCAACTGGACTCTTGCAAATAGTGGAGAACAACTGCATGGCTCTCATGAACTGGAAAA TCATCAGGTTGCCGAGTCTATGGAATACGGATTCCTGCCCAATCCAGTAGCTGTTAAATCTCTATCTGAA TCCCCATTCAAAGTTCATTTGGAAAAACTCGGCTTGAGACAGAGGAAGCTGGATGAGGATCTGCAGTTAT ACCAGACACCTCTAGAGCTCAAATTAAAACATAGCACTGTGCATGTAGATGAGCTGTGTCCTCTCATCGT CCCCAATCCTGGGGTTTCAGTCATTCATGGTTATGCAGATTGGGTTAAGAAATCACCCAGAGATTTACTG GAACTACCAATTGTGAAGCACTGGAGCCTGACGTGGACATTATGTGAAGCCCTATGGGGCCACCTGAAGG AGCTTGACAGCCAGCTGGATGAGCCCAGTGAATACATTCAGACTCTGGAGCGGAGAAGAGCTTTTTCCCG CTGGCTGTCCCACACTGCTGCACCTCAGATAGAAGAGGAAGTCTCCTTAACCCGCAGAGACAGTCCCATA GAGGCTGTCTTCAGCTACCTCACGGGCAGTAGAATTAGTGAGGCCTGTTGTCTGGCACAGCAGTCAGGTG ATCATCGCCTTGCCCTTCTTCTGTCTCAGCTGGTGGGAAGCCAGTCAGTCCGGGAGCTGCTCACCATGCA GTTGGCTGATTGGCATCAGCTCCAGGCTGACTCCTTCATCCACGATGAGCGGTTGCGCATATTTGCCCTG TTAGCTGGAAAACCGGTGTGGCAGCTCTCAGAACAGAAACAAATCAATGTATGCTCCCAGCTAGATTGGA AACGAACACTTGCTATCCATCTTTGGTATTTGCTTCCACCAACTGCCTCCATTTCCAGGGCTCTCAGCAT GTATGAAGAAGCATTTCAGAATACTTGTGAGGGTGATAAATATGCCTGTCCCCCTCTTCCTTCTTACCTG GAGGGCAGTGGCTGTGTGGTAGAGGAAGAAAAAGACCCCCAGAGACCACTTCAAGACGTTTGCTTTCATC TTCTAAAGCTCTACAGTGACAGACATTATGGTCTCAATCAGCTCTTGGAACCCCGAAGCATAACTGCAGA TCCTTTGGACTACCGCCTAAGCTGGCACCTTTGGGAAGTGCTCCGTGCTCTTAACTATACACATCTCTCA GAACAGTGTGAGGGCGTGCTACAGGCCAGTTATGCTGGCCAGCTGGAAAGTGAAGGACTCTGGGAGTGGG CCATCTTTGTCTTTCTGCACATTGACAACTCAGGCATGCGTGAGAAGGCTGTTCGAGAACTGCTGACCCG GCACTGCCAACTCTCAGAGACCCCTGAGTCTTGGGCTAAGGAGACTTTCCTTACTCAGAAGCTTTGTGTG CCTGCTGAGTGGATTCATGAGGCCAAAGCAGTTCGTGCACACATGGAATCCAATAAGCACTTGGAGGCCC TCTATTTATTTAAAGCTGGTCACTGGAACCGCTGCCACAAACTAGTCGTTCGGCACCTAGCTTCTGATGC CATTATTAATGAGAACTATGACTACCTGAAAGGTTTCTTAGAAGATCTGGCACCTCCAGAGCGCAGCAGC CTAATTCAGGACTGGGAAACATCTGGGCTTGTTTACTTGGATTACATTCGAGTAATTGAAATGCTCCACC GTATTCAGCAGGTGGATTGCTCAGGCTATGAACTAGAGCACTTACATACCAAAGTGACTTCACTGTGTAA CCGGATAGAGCAGATTCCGTGCTACAATGCCAAGGATCGCCTGGCTCAGTCAGATATGGCCAAGCGGGTA GCCAACTTACTGCGGGTAGTTCTGAGCCTTCAGCACACTCCTGACGCCACCTCCAACTCCACTCCGGACC CTCAGCGAGTCCCTTTGCGTCTTTTGGCTCCCCACATTGGCAGGCTCCCTATGCCTGAGGACTATGCCTT GGAGGAACTGCGAGGCCTTACCCAGTCCTACCTTCGAGAACTGACTGTTGGGAGCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200873 representing NM_031074 Red=Cloning site Green=Tags(s) MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQ PATSTSTGFGFGTSTGTSNSLFGTANTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPF GNTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQA NRKGPQNQVGAGTTTGLFGSSPATSSATGLFSSSTTNSAFSYGQNKTAFGTSTTGFGTNPGGLFGQQNQQ TTSLFSKPFGQATTTPNTGFSFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLF GQPNTGFGAVGSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNNSGSSIF GSKPAAGTLGTGLGTGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTSTAILGFGAPQAPVALTD PNASAAQQAVLQQHLNSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRV RPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNHDSEDLASPSEYPEN GERFSFLSKPVDENHQQDGDDDSLVSRFYTNPIAKPIPQTPESAGNKNNSSSNVEDTFIALNMRAALRNG LEGSSEETSFHDESLQDDRDEIENSAFQIHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRK GYGSIYFEGDVNLTNLNLDDIVHIRRKEVIVYVDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSP DRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPPKTTSKKLKTAPLP PAGQATTFQMTLNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVPDSVLEESVPEDQEPVSASTQI ASSLGINPHVLQIMKASLLVDEEDVDAMEQRFGHFPSRGDTAQEICSPRLPISASHSSKSRSIVGGLLQS KFASGTFLSPSASVQECRTPRTSSLMNVPSTSPWSVPLPLATVFTVPSPAPEVPLKTVGIRRQPGLVPLE KSITYGKGKLLMDMALFMGRSFRVGWGPNWTLANSGEQLHGSHELENHQVAESMEYGFLPNPVAVKSLSE SPFKVHLEKLGLRQRKLDEDLQLYQTPLELKLKHSTVHVDELCPLIVPNPGVSVIHGYADWVKKSPRDLL ELPIVKHWSLTWTLCEALWGHLKELDSQLDEPSEYIQTLERRRAFSRWLSHTAAPQIEEEVSLTRRDSPI EAVFSYLTGSRISEACCLAQQSGDHRLALLLSQLVGSQSVRELLTMQLADWHQLQADSFIHDERLRIFAL LAGKPVWQLSEQKQINVCSQLDWKRTLAIHLWYLLPPTASISRALSMYEEAFQNTCEGDKYACPPLPSYL EGSGCVVEEEKDPQRPLQDVCFHLLKLYSDRHYGLNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLS EQCEGVLQASYAGQLESEGLWEWAIFVFLHIDNSGMREKAVRELLTRHCQLSETPESWAKETFLTQKLCV PAEWIHEAKAVRAHMESNKHLEALYLFKAGHWNRCHKLVVRHLASDAIINENYDYLKGFLEDLAPPERSS LIQDWETSGLVYLDYIRVIEMLHRIQQVDCSGYELEHLHTKVTSLCNRIEQIPCYNAKDRLAQSDMAKRV ANLLRVVLSLQHTPDATSNSTPDPQRVPLRLLAPHIGRLPMPEDYALEELRGLTQSYLRELTVGSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031074
ORF Size	5448 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031074.2, NP_112336.2
RefSeq Size	6595 bp
RefSeq ORF	5451 bp
Locus ID	81738
UniProt ID	P49793
MW	197.3 kDa
Gene Summary	nuclear pore complex protein; involved in nuclear pore complex docking and transport [RGD, Feb 2006]

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