Trps1 (NM_001134837) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202101 representing NM_001134837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGGAAAAAGAACCCCCCTCTGAGAAACGTTGCTAGTGAAGGCGAGGGTCAAACCCTGGAGCCAA CAGCTACAGAGAGCAAGGCATCTGGGAAGAACAAAGAATTTTCTGCAGATCAGATGTCAGAAAATACAGA TCAGAGTGATGTTGCAGAATTGAACACGAAGGAGGAACACAGCACGCATGGCCAAGAGCCGTCTTCGAGC AGTAAGAAGGACTTGCAAATCTCAGGCCTGAGTGAGAAGGCTGGCTTCAATTATGAAAGCCCCAGTAAGG GAGGGAACCTTGCCTCCTTCCCACATGATGAGGTAACAGACAGAAATATGTTGGCTTTCTCATCTCCAGC TGCTGGGGGAGTCTGCGAGCCCTTGAAGTCTCCTCAAAGAGCAGAGGCAGATGACCCTCAAGATATGGCC TGCACCCCATCAGGGGATTCACTGGAGACAAAGGAAGATCATAAAATGTCACCAAAGGCCACTGAGGAAA CAGGGCCAGTGCAGAGTGGTCAAGCCAATTGTCAAGGTTTGAGTCCAGTTTCCGTGGCCTCAAAAAACCC ACAAGTGCCTTCAGATGGGGGTGTCAGACTGAGTAAACCCAAAAGTGACTTACTGGTGAGTGACAACCCA GACCCGGCACCTCTGTCTCCAGAGCTTCAGGACTTTAAATGCAATATCTGTGGATACGGTTACTATGGCA ATGACCCCACAGATCTGATTAAGCACTTCCGAAAGTATCACTTAGGACTGCATAATCGCACCAGGCAGGA TGCTGAGCTGGACAGCAAAATCTTGGCCCTTCATAACATGGTGCAGTTCAGCCATTCCAAAGACTTCCAG AAGGTCAACCGTTCTGTGCTTTCTGGCGTGCTGCAGGATATCAGCTCTTCACGGCCTGCGCTACTAAATG GGACCTATGATGTACAGGTCACTTCAGGTGGAACGTTCATTGGCATTGGACGGAAAACTCCCGATTGCCA AGGCAACACCAAGTATTTCCGCTGCAAATTCTGCAATTTCACTTACATGGGCAACTCGTCAACTGAACTA GAACAACATTTTCTTCAGACTCACCCGAATAAAATCAAAGTGTCTCTCCCGTCCTCCGAGGGCGTGAAAG CTTCAGAGAAAAACTCTAACAAATCCATCCCTGCGCTTCGAGCCAGTGACTCTGGGGATGTAGGAAAATG GCAGGACAAAATGACAGTCAAAGCTGGAGATGACACCCCTGTCGGCTACTCAGTGCCCATCAAGCCCCTC GATTCCTCACGACAAAATGGTACAGAGGCCACCAGTTATTACTGGTGTAAATTTTGTAGTTTCAGCTGTG AGTCATCTAGCTCGCTTAAGCTGCTAGAACATTACGGCAAGCAGCATGGAGCAGTGCAGTCGGGCGGCCT TAATCCAGAGTTGAATGACAAACTTCCCAGGGGCTCTGTCATTAATCAAAATGATCTAGCCAAAAGTGTA GAAGGAGAGCCATTGACCAAGCCAGAAAAGGGCTTGAGTGGGGCTAAAAAGAAGGACTTCCCCAGCAAGG GAGCAGAGGATAATATGGTAACGAGCTATAACTGTCAGTTCTGTGACTTTAGATATTCCAAAAGCCATGG CCCTGATGTGATTGTAGTGGGTCCACTTCTCCGTCATTATCAGCAACTCCATAACATCCACAAGTGTACC ATTAAGCATTGTCCATTCTGTCCCAGAGGCCTTTGCAGCCCAGAAAAGCACCTTGGAGAAATTACTTATC CGTTTGCTTGTAGAAAAAGTAATTGTTCCCACTGTGCACTCTTGCTGTTACACTTGTCTCCTGGGGTGGC CGGAAGTTCCAGAGTCAAACACCAATGCCACCAGTGTTCCTTCTCTACCCCCGATGTAGATGTGCTCCTC TTTCACTATGAGACCATGCATGAGTCCCAAGCATCGGATGTCAAACAAGAGGTAAACCACCTGCTAGGAT CTGAAGGGCAGCAGGCTGCCAGGGACAGCAAGGAACACTCGTGCACCAAATGTGATTTTATAACCCAGGT GGAAGAAGAGATCTCTAGACACTACAGGAGAGCACACAGCTGCTATAAATGTCGTCAGTGCAGTTTCACA GCTGGTGACACTCAGTCACTGTTGGAGCACTTCAACACGGTTCACTGCCAGGAACAGGAAGTAGCTACTG CTAATGGCGAAGAGGATGGCCATGCCATACCCACCATCAAGGAAGAGCCCAAAATTGATCTCAAGGTCTA CAGTCTGCTAAACCCAGACTCTAAAATGGGAGAGACAGTTCCCGAGAGCATAGTTAAGAGAGAGAAACTT GATGACAAGGAAGGGCTAAAAGATAAAATCTGGACGGAGAGTTCAACTGATGACCTTCGTGGTGTGACTT GGAGAGGAGCCGACATCCTACGGGGTAGTCCATCCTATACTCAAGCAAGTCTGGGGCTCTTGACGCCTGT GTCCAGCTCCCAAGAGCAGACAAAGACTCTGAGGGACAGCCCCAATGTCGAAGCTGCCCACCTGGCCCGA CCTATGTATGGCTTGGCTGTCGATACCAAGGGCTTCCTGCAAGGGGCTCCTGCTGGTGGCGAGAAGTCTG CATCTCTCACTCAGCAGTATCCTGCTTCTGGAGAGGGCAAGACCAAGGACGAATCCCAGTCGCTGTTACG GAGGCGAAGAGGCTCTGGTGTTTTTTGTGCCAATTGCCTGACCACAAAGACCTCTCTCTGGCGAAAGAAT GCAAATGGCGGATATGTATGCAACGCATGTGGCCTCTACCAGAAACTTCACTCGACTCCCAGGCCTTTAA ACATCATTAAACAAAACAACGGCGAGCAGATTATTAGAAGACGGACAAGAAAGCGCCTTAACCCAGAGGC ACTTCAGGCTGAGCAACTCAACAAACAGCAGAGGGGAAGTGGGGAGGAGCAGGTCAATGGAAGCCCCTTA GAGAGGAGGTCAGAAGATCACTTAAGTGAAAGTCATCAGAGAGAAATTCCACTTCCGAGCCTGAGTAAAT ACGAAGCCCAGGGTTCATTGACTAAAAGCCATTCTGCTCAACAGCCGGTCCTGGTCAGCCAAACTCTGGA TATTCACAAAAGGATGCAGCCTTTGCACATTCAGATAAAAAGTCCTCAGGAAAGTACTGGAGATCCAGGA AACAGTTCATCCGTATCTGATGGGAAAGGAAGTTCTGAAAGAGGCAGCCCCATAGAAAAGTACATGAGGC CTGCAAAACACCCCAACTATTCTCCACCAGGCAGCCCCATCGAAAAGTACCAATACCCACTTTTTGGAGT TCCTTTTGTACATAATGACTTCCAGAGTGAAGCTGATTGGCTACGGTTCTGGAGTAAATATAAGCTCTCT GTTCCTGGGAATCCGCACTACTTGAGTCATGTGCCTGGCTTACCAAATCCTTGCCAAAACTATGTGCCTT ATCCCACCTTCAATCTGCCTCCTCATTTCTCAGCTGTTGGATCAGACAATGACATTCCTCTAGATTTGGC GATCAAGCATTCCAGACCTGGGCCAACTGCAAATGGTGCCGCCAAGGAGAAAAGCAAGGCACCATCAAAC GTAAAAAATGAAGGTCCCTTGAATGTAGTTAAGACGGAGAAAGTTGACAGAAGCACTCAAGATGAACTTT CAACGAAATGTGTGCACTGTGGCATTGTTTTCCTGGATGAAGTGATGTATGCTTTGCATATGAGTTGCCA TGGTGACAGTGGACCTTTCCAGTGCAGCATATGCCAGCATCTTTGCACGGACAAATATGACTTCACAACA CATATCCAGAGGGGCCTGCATAGGAACAATGCACAAGCAGAAAAAAATGGAAAACCTAAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202101 representing NM_001134837 Red=Cloning site Green=Tags(s) MVRKKNPPLRNVASEGEGQTLEPTATESKASGKNKEFSADQMSENTDQSDVAELNTKEEHSTHGQEPSSS SKKDLQISGLSEKAGFNYESPSKGGNLASFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMA CTPSGDSLETKEDHKMSPKATEETGPVQSGQANCQGLSPVSVASKNPQVPSDGGVRLSKPKSDLLVSDNP DPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQ KVNRSVLSGVLQDISSSRPALLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTEL EQHFLQTHPNKIKVSLPSSEGVKASEKNSNKSIPALRASDSGDVGKWQDKMTVKAGDDTPVGYSVPIKPL DSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLPRGSVINQNDLAKSV EGEPLTKPEKGLSGAKKKDFPSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLHNIHKCT IKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGVAGSSRVKHQCHQCSFSTPDVDVLL FHYETMHESQASDVKQEVNHLLGSEGQQAARDSKEHSCTKCDFITQVEEEISRHYRRAHSCYKCRQCSFT AGDTQSLLEHFNTVHCQEQEVATANGEEDGHAIPTIKEEPKIDLKVYSLLNPDSKMGETVPESIVKREKL DDKEGLKDKIWTESSTDDLRGVTWRGADILRGSPSYTQASLGLLTPVSSSQEQTKTLRDSPNVEAAHLAR PMYGLAVDTKGFLQGAPAGGEKSASLTQQYPASGEGKTKDESQSLLRRRRGSGVFCANCLTTKTSLWRKN ANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSGEEQVNGSPL ERRSEDHLSESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQESTGDPG NSSSVSDGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGVPFVHNDFQSEADWLRFWSKYKLS VPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGAAKEKSKAPSN VKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKYDFTT HIQRGLHRNNAQAEKNGKPKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134837
ORF Size	3843 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134837.1, NP_001128309.1
RefSeq Size	4577 bp
RefSeq ORF	3846 bp
Locus ID	299897
MW	141 kDa

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