Pard3 (NM_031235) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Par3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202843 representing NM_031235 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGACCGTGTGCTTCGGGAGGACCCGGGTGGTCGTGCCGTGCGGAGATGGCCGCATGAAAGTTT TCAGCCTTATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCGTGGCCAAGGATCCAAACTACTGGATACA GGTACATCGCTTGGAGCATGGGGACGGTGGGATTCTAGACCTGGATGACATCCTCTGTGACGTTGCCGAT GACAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCCCACCATGGAGGAGATGGTACGAGTGCCA GCTCCACGGGAACCCAGAGTCCAGAGATATTCGGCAGTGAGCTGGGCACCAACAATGTTTCTGCTTTTCG GCCTTATCAAACCACAAGTGAAATTGAGGTCACGCCTTCAGTCCTTCGGGCAAATATGCCTCTTCACGTC CGACGGAGCAGTGACCCAGCTTTAACTGGCCTATCCACTTCCGTCAGTGATAACAACTTTTCCTCAGAGG AGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCAGAACACCACTGGAAG TCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGGAGCCTCCCACGGGATCCCAGTAGCTGGTCC AACCAGTTCCAGAGAGACAACGCCCGCTCCTCTCTGAGTGCCAGCCACCCAATGGTAGACCGGTGGCTGG AGAAGCAAGAACAGGACGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCCGGTTGGACATGCTGA TACTGGACTGGAGAATATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTACAAGTCCCCAACGAT GGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACACTGGGGTTGTTAGTGA AGCGGTTGGAGAAAGGTGGGAAGGCTGAACAAGAAAACCTTTTCCACGAGAATGACTGCATTGTGAGGAT TAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAGCATATGTTCCGCCAAGCCATGCGTGCG CGAGTCATTTGGTTCCACGTGGTCCCTGCAGCGAACAAGGAGCAGTATGAACAGCTCTCCCAACGAGAGA TGAACAACTATTCCCCTGGCCGCTTCAGCCCCGACAGCCACTGTGTGGCCAACAGGAGTGTGGCCAACAA TGCGCCTCAGGCGTTGCCCAGAGCACCCAGACTGAGCCAGCCACCTGAGCAGCTGGATGCTCACCCCCGA CTACCTCATAGTGCTCATGCCTCAACCAAACCGCCCACAGCCCCAGCCTTGGCTCCACCGAATGTGCTTA GTACCAGTGTCGGCAGTGTGTACAACACGAAGAGAGTAGGCAAGAGGCTCAACATTCAGCTTAAAAAAGG TACAGAAGGACTGGGATTTAGCATCACTTCCCGAGACGTAACTATAGGTGGCTCAGCTCCCATTTATGTC AAGAACATTCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGGCTCAAGGCGGGAGACCGGCTAATAGAGG TCAATGGAGTAGATTTAGCGGGCAAGTCCCAGGAGGAAGTTGTTTCCCTGTTGAGAAGCACCAAGATGGA GGGGACCGTGAGCCTTCTGGTCTTTCGTCAAGAAGAGGCTTTCCACCCAAGGGAAATGAATGCCGAACCC AGCCAGATGCAGAGTCCAAAAGAAACGAAAGCCGAAGACGAGGACATTGTTCTCACACCTGACGGTACCA GGGAGTTTCTGACTTTCGAAGTTCCACTGAATGACTCAGGGTCTGCAGGGCTTGGTGTCAGCGTCAAGGG GAACCGCTCCAAAGAGAACCATGCAGATTTGGGGATCTTCGTTAAGTCCATCATTAACGGGGGAGCTGCA TCTAAAGATGGGAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAACGGAGAGTCTCTGCTGGGCAAAG CCAACCAGGAAGCCATGGAGACTCTGAGGAGGTCCATGTCCACCGAGGGCAACAAGCGTGGCATGATCCA GCTCATCGTGGCGAGGCGGATCAGCAGATGTAACGAGTTGCGGTCTCCTGGGAGCCCCGCTGCACCCGAG CTGCCCATAGAAACAGAGTTGGATGACAGAGAACGCAGGATCTCACACTCCCTCTACAGTGGGATCGAGG GGCTGGATGAGTCTCCCACCAGGAATGCCGCACTCAGCAGGATAATGGGTGAGTCAGGTAAATGCCAGCT CTCCCCAACCGTGAACATGCCACATGATGACACTGTCATGATTGAAGATGACAGGCTGCCTGTGCTCCCT CCTCACCTCTCTGACCAGTCCTCCTCCAGCTCCCATGATGACGTGGGATTCATAATGACAGAAGCAGGCA CGTGGGCCAAGGCTACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGTTCTCGC TTTTCAACGAGAAGGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAAATGCCAGT CAATTGGATTTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGGTATAGCTGACGAGACTAAACTCA ATACAGTGGATGACCAGAGAGCAGGCTCCCCCAATAGAGATGTGGGACCCTCCTTGGGTCTGAAGAAATC CAGCTCTTTAGAAAGTCTGCAGACGGCTGTTGCTGAGGTGACCCTGAATGGGAACATTCCTTTCCACCGC CCACGGCCACGAATCATCCGAGGAAGGGGCTGCAACGAGAGCTTCAGAGCCGCCATTGACAAGTCCTACG ATAAGCCCATGGTGGATGACGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAG GTCAGGGAGGGAGTCCGTGTCCACGTCCAGTGATCAGCCTTCCTATTCTCTGGAGAGACAAATGAATGGA GACCCAGAGAAGAGGGACAAGGCAGAGAAGAAAAAGGACAAAGCCGGAAAGGATAAGAAGAAAGACCGAG AGAAGGAGAAGGATAAACTGAAAGCCAAGAAGGGGATGCTGAAAGGCTTGGGGGACATGTTCAGGTTTGG CAAACATCGAAAAGATGACAAGATGGAAAAAATGGGTCGAATAAAAATCCAGGACTCTTTCACCTCAGAA GAGGACAGGGTGCGGATGAAGGAGGAACAGGAGAGGATTCAAGCCAAAACTCGAGAATTTAGGGAGCGGC AAGCTCGAGAGCGCGACTATGCTGAAATCCAAGATTTCCATCGAACATTTGGCTGTGATGACGAGTTGCT GTATGGGGGCATGTCATCCTATGATGGCTGCCTGGCTCTCAATGCCAGACCCCAGAGCCCAAGAGAAGGT CACCTGATGGACACTTTGTATGCACAAGTAAAGAAACCTCGGAGCTCCAAACCTGGGGACAGCAATCGAT CCACCCCTAGCAACCATGACCGGATACAACGTCTACGGCAAGAATTCCAGCAAGCCAAACAGGATGAGGA CGTGGAAGACCGGCGCCGTACCTACAGCTTTGAGCAATCTTGGTCCAGCTCACGGCCAGCATCGCAGAGT GGTCGGCACTCGGTGTCAGTGGAGGTTCAAGTGCAGCGGCAGCGCCAGGAGGAGCGAGAGAGCTTCCAGC AGGCCCAGCGCCAGTACAGCTCCCTGCCAAGACAAAGCAGGAAGAATGCCAGCTCTGTATCACAGGATTC CTGGGAACAGAACTACGCCCCTGGTGAAGGCTTCCAGAGTGCCAAGGAGAACCCCAGGTATTCCAGTTAC CAGGGCTCCAGGAACGGCTATCTAGGTGGCCATGGCTTTAATGCCAGGGTCATGCTGGAGACCCAGGAGC TTCTCCGACAGGAGCAGAGGCGAAAAGAGCAGCAACTGAAGAAGCAGCCACCAGCGGATGGAGTCAGAGG GCCCTTCCGGCAAGATGTACCCCCGTCTCCATCTCAGGTGGCCAGGCTGAACAGACTGCAGACACCCGAG AAAGGGCGGCCCTTCTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202843 representing NM_031235 Red=Cloning site Green=Tags(s) MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVAD DKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHV RRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWS NQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPND GGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRA RVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPR LPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYV KNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEP SQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAA SKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPE LPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLP PHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNAS QLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHR PRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNG DPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSE EDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREG HLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQS GRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSY QGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPE KGRPFYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031235
ORF Size	4011 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031235.1, NP_112514.1
RefSeq Size	5500 bp
RefSeq ORF	4014 bp
Locus ID	81918
UniProt ID	Q9Z340
MW	149.4 kDa
Gene Summary	may mediate the establishment and maintenance of epithelial cell polarity [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.