Flt4 (NM_053652) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Vegfr3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205561 representing NM_053652 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGGCGCTGCGCTGAACCGGCGCCTGTGGCTCTGCCTCGGACTCCTCCAAGGCCTGGCAAATG GCTACTCCATGACCCCTCCAACTCTGAACATCACAGAGGATTCATATGTCATTGACACCGGGGACAGCCT ATCCATATCCTGCAGGGGGCAGCACCCCCTCGAGTGGACCTGGCGAGGGGCCCAAGAGGTACTGACCACA GGTGGGAAGGACAGCGAGGACACGCAGGTTGTGCAAGACTGTGAAGGCACAGAAGCTAGGCCCTACTGCA AGGTGCTGTCGCTGGCCCAGACTCACGCCAACAACACGGGCAGCTATTACTGCTACTACAAGTATATCAA GGCCCGGATTGAGGGCACCACAGCTGCCAGCACCTATGTGTTTGTAAGAGACTTTGAACAGCCTTTCATC AACAAACCTGACACGCTCCTGGTCAACAGGAAGGACTCGATGTGGGTGCCCTGCTTGGTGTCCATTCCCG GCCTCAACATCACACTGCGCTCGCAAAGTTCGGTGCTGCACCCTGATGGGCAGGAGGTGTTGTGGGACGA CCGCCGGGGCATGCGGGTGCCCACTCTACTGTTGCGTGACGCCCTGTACCTGCAGTGCGAGACCACCTGG GGAGACCAGGACTTCCTTTCCAATCCCTTCCTCGTGCACATCACAGGCAATGAGCTCTATGACATCCAGC TGTACCCCAAGAAGTCACTGGAGCTGTTGGTTGGAGAGAAGCTGGTTTTGAACTGTACAGTATGGGCTGA GTTCGACTCTGGTGTCACCTTCGACTGGGATTACCCAGGAAAGCAGGCAGAGCGTGCTAAGTGGGTACCT GAGCGGCGTTCCCAGCAGACCCACACGGAACTCTCCAGCATCCTGACCATCCACAATGTCAGCCAGCACG ACCTGGGCCCCTATGTGTGTGAGGCCAACAATGGGATTCAGCAGTTCCGGGAAAGCACGGAGGTCATTGT GCACGAAAAGCCCTTCATCAGTGTCGAGTGGCTCAAAGGACCTGTCCTGGAGGCCACAGCCGGTGACGAG ATGGTGAAGCTACCCGTGAAGCTTGCAGCTTATCCCCCACCGGAGTTCCAATGGTACAAGGACAGAAAGG CAGTGACTGGGCGCCACAATCCACATGCTCTGGTGCTCAAAGAGGTGACCGAGGCCAGTGCAGGCGTCTA CACCCTCGCCCTGTGGAACTCTGCAGCCGGTCTGAGGCAGAATATCAGTCTGGAGCTGGTGGTGAATGTG CCGCCCCACATCCATGAAAAGGAAGCTTCTTCACCCAGCATCTACTCCCGCCACAGCCGCCAGACCCTCA CCTGCACCACCTATGGGGTACCCCAACCTCTCAGTGTTCAGTGGCACTGGAGGCCCTGGACACCCTGTAA GACATTTGCCCAGCGCAGTCTCCGGAGGCGGCAGCCGCGGGATGGCATGCCACAGTGCCGAGACTGGAAG GAGGTGACCACTCAGGACGCTGTGAACCCCATCGAGAGCCTGGACACCTGGACCGAGTCTGTGGAGGGCA AGAACAAGACGGTGAGCAAGCTGGTGATTCAGGATGCCAATGTGTCAGCCATGTACAAATGCGTGGTCTT CAACAAAGTGGGCCAGGACGAGCGGCTCATCTACTTCTATGTGACCACCATCCCTGACGGCTTCAGTATC GAATCAGAGCCATCCGAGGATCCCTTAGAAGGCCAGTCCGTGCGCCTCAGCTGCCGGGCGGACAACTACA CGTATGAGCATCTGCGCTGGTACCGGCTCAACCTCTCCACGCTGCACGACGCTCAAGGGAACCCCCTACT GCTCGACTGCAAGAACGTGCACCTGTTTGCCACGCCCCTAGAGGCCAACCTAGAGGAGGCGGAGCCTGGG GCCCGCCACGCCACCCTCAGCTTGAATATCCCCCGAGTGGCGCCCGAGGACGAGGGTGACTACGTGTGTG AAGTGCAGGATAGGCGCAGCCAGGACAAGCACTGCCACAAGAAGTACCTGTCCGTGCAGGCCCTGGAAGC TCCTCGGCTCACGCAGAACTTAACCGACCTCCTGGTGAACGTGAGAACCTCCCTGGAGATGCGATGTCCG GTAGCCGGAGCACATGTGCCCAGTATTGTGTGGTACAAAGATGAAAGGCTCCTGGAGAAAGAGTCGGGAA TCGACCTGGCAGACTCCAATCAGAGGCTGAGCATCCAACGCGTGCGCGAGGAGGACGCAGGTCGTTACCT GTGCAGTGTGTGCAATGCCAAGGGCTGCGTAAACTCCTCCGCCAGCGTGGCCGTAGAAGGCTCGGAAGAT AAAGGCAGCATGGAGATTGTGATACTCATTGGCACTGGCGTCATCGCAGTCTTCTTCTGGGTCCTCCTCC TGCTCATCTTCTGTAACATGAAAAGGCCTGCCCATGCAGACATCAAGACGGGCTACCTGTCCATCATCAT GGACCCCGGGGAGGTGCCTTTGGAGGAGCAGTGTGAATACCTGTCCTATGACGTCAGCCAGTGGGAGTTC CCCAGGGAAAGGTTGCACCTCGGGAGAGTCCTAGGCCATGGGGCTTTTGGGAAGGTGGTGGAAGCCTCAG CCTTTGGCATCAATAAAGGCAGCAGCTGTGATACCGTGGCGGTGAAGATGCTGAAAGAGGGCGCTACTGC CAGCGAACACCGTGCCCTGATGTCCGAGCTCAAGATCCTAATTCACATCGGTAACCACCTCAATGTGGTC AACCTCCTAGGGGCGTGCACCAAGCCCAATGGCCCTCTCATGGTGATCGTGGAGTTTTGCAAATACGGCA ACCTCTCCAACTTCTTGCGTGTCAAGCGGGAGACGTTCGACCCCTACGCAGAGAAGTCTCCCGAGCAACG CAGGCGCTTCCGCGCGATGGTAGAAGGCGCCAAGGCTGATAGGAGGAGACTTGGAAGCACCGACAGAGCC CTGTTTACAAGGTTCCTGATGGGCAAAGGAAGTGCACGGCGAGCCCCATTTGTCCAAGAAGCTGAGGACC TGTGGTTGAGCCCGCTGACCATGGAGGACCTTGTCTGCTACAGTTTCCAAGTGGCCCGGGGAATGGAGTT CCTGGCTTCCCGCAAGTGCATTCACAGAGACCTGGCTGCTCGGAACATTTTACTGTCAGAAAGTGACATA GTGAAGATCTGTGACTTTGGCCTCGCTCGGGACATCTACAAAGACCCTGACTATGTCCGAAAGGGCAGCG CCCGACTGCCTCTGAAATGGATGGCCCCCGAGAGCATCTTTGATAAGGTGTACACCACGCAGAGTGATGT GTGGTCCTTCGGCGTGCTGCTGTGGGAGATCTTCTCATTGGGGGCCTCTCCATACCCTGGGGTACAGATC AATGAGGAATTCTGCCAGCGGCTGAAAGACGGCACACGAATGAGGGCCCCAGAACTGGCCACTCCTGCCA TACGCCACATCATGCAGAGTTGCTGGTCTGGAGACCCTAAAGCGAGGCCTGCTTTCTCTGACCTAGTGGA GATCCTGGGGGACCTGCTTCAGGGTGGAGGCTGGCAGGAGGAGGAGGAGGAATGCATGGCCCTGCACAGT TCTCAGAGCTCAGAGGAGGATGGCTTCATGCAGGCATCCACCACAGCTCTACATATCACGGAGGCTGACG CTGAGAGCAGTCCACCCAGCATGCATTGCCACAGCCTGGCAGCCAGATATTACAACTGTGTGTCCTTTCC TGGGCGCCTGGTCAGGGGGACTAAGGCTCCAGGTTCCTCCAGGATGAAGACGTTTGAAGAATTGCCCATG ACCCCTACAACCTACAAAGCCTCTGTGGATAACCAAACAGACAGCGGGATGGTGCTGGCCTCAGAAGAGT TTGAGCAGATAGAAAGCAGGCATAGACAAGAAGGCAGCTTCAGCCGTAAAGATCCTGGCCAGCACATGGA TATTTCCAGAGGACACCCGGACCTCCAGGGGAGGCGGCGACGGCCCACCCAGGGGGCACAAGGAGGCAAG GTGTTTTACAACAACGAGTATGGGGAGGTCTCCCAGCCGTGTACAGAAGGTGACTGCTGCCCGTCTGCTG GCTCCACCTTCTTCGCAGACAGCAACTACGCTCCACCTTCTTCGCAGACAGCAACTAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205561 representing NM_053652 Red=Cloning site Green=Tags(s) MQPGAALNRRLWLCLGLLQGLANGYSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWRGAQEVLTT GGKDSEDTQVVQDCEGTEARPYCKVLSLAQTHANNTGSYYCYYKYIKARIEGTTAASTYVFVRDFEQPFI NKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSVLHPDGQEVLWDDRRGMRVPTLLLRDALYLQCETTW GDQDFLSNPFLVHITGNELYDIQLYPKKSLELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVP ERRSQQTHTELSSILTIHNVSQHDLGPYVCEANNGIQQFRESTEVIVHEKPFISVEWLKGPVLEATAGDE MVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNV PPHIHEKEASSPSIYSRHSRQTLTCTTYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQPRDGMPQCRDWK EVTTQDAVNPIESLDTWTESVEGKNKTVSKLVIQDANVSAMYKCVVFNKVGQDERLIYFYVTTIPDGFSI ESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPG ARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVRTSLEMRCP VAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSED KGSMEIVILIGTGVIAVFFWVLLLLIFCNMKRPAHADIKTGYLSIIMDPGEVPLEEQCEYLSYDVSQWEF PRERLHLGRVLGHGAFGKVVEASAFGINKGSSCDTVAVKMLKEGATASEHRALMSELKILIHIGNHLNVV NLLGACTKPNGPLMVIVEFCKYGNLSNFLRVKRETFDPYAEKSPEQRRRFRAMVEGAKADRRRLGSTDRA LFTRFLMGKGSARRAPFVQEAEDLWLSPLTMEDLVCYSFQVARGMEFLASRKCIHRDLAARNILLSESDI VKICDFGLARDIYKDPDYVRKGSARLPLKWMAPESIFDKVYTTQSDVWSFGVLLWEIFSLGASPYPGVQI NEEFCQRLKDGTRMRAPELATPAIRHIMQSCWSGDPKARPAFSDLVEILGDLLQGGGWQEEEEECMALHS SQSSEEDGFMQASTTALHITEADAESSPPSMHCHSLAARYYNCVSFPGRLVRGTKAPGSSRMKTFEELPM TPTTYKASVDNQTDSGMVLASEEFEQIESRHRQEGSFSRKDPGQHMDISRGHPDLQGRRRRPTQGAQGGK VFYNNEYGEVSQPCTEGDCCPSAGSTFFADSNYAPPSSQTAT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053652
ORF Size	4089 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	4360 bp
RefSeq ORF	4092 bp
Locus ID	114110
UniProt ID	Q91ZT1
MW	153.3 kDa
Gene Summary	VEGF receptor-related, cell surface associated kinase with growth promoting ability [RGD, Feb 2006]

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