Ambra1 (NM_001134341) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nyw1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206199 representing NM_001134341 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTTGTCCCAGAGAAGAATGCTGTACGAATCCTCTGGGGCCGAGAGCGGGGCACTCGTGCCATGG GTGCCCAGCGGCTTCTGCAGGAGCTGGTGGAGGATAAGACTCGATGGATGAAATGGGAGGGCAAGAGAGT AGAACTTCCTGATAGTCCACGCTCGACCTTCTTACTGGCCTTCAGCCCAGACAGGACTCTCTTGGCGTCC ACACATGTGAACCATAACATCTATATTACAGAGGTTAAGACTGGCAAGTGTGTTCACTCTCTGATTGGAC ACCGCCGTACTCCATGGTGTGTCACTTTTCATCCCACTATCTCAGGCCTTATTGCTTCTGGCTGCCTAGA TGGGGAAGTTAGGATTTGGGACTTACATGGTGGCAGTGAAAGCTGGTTCACAGATAGCAACAATGCCATA GCTTCTTTGGCTTTCCACCCTACGGCTCAGCTCCTGCTGATCGCCACTGCAAATGAGATCCATTTCTGGG ATTGGAGTCGTCGGGAACCCTTTGCTGTGGTGAAAACAGCTAGTGAGATGGAGCGTGTTCGTCTGGTGAG ATTTGATCCACTTGGACACTACTTACTTACAGCTATTGTTAACCCCTCTAATCAGCAGGGTGATGATGAA CCAGAAATCCCTATAGATGGAACAGAATTATCTCATTACCGTCAGCGTGCCCTCCTGCAATCACAGCCAG TTCGCCGGACACCCCTCCTCCACAATTTCCTGCATATGCTGTCCTCCCGCTCCTCTGGCATCCAGGTGGG AGAGCAAAGCACAGTACAAGATTCTGCTACCCCCTCACCCCCACCGCCTCCCCCTCAGCCCTCCACGGAG CGTCCCAGGACTTCCGCTTACATCAGACTCCGACAGCGGGTCAGTTACCCCACCACAGTTGAGTGCTGCC AGCACCCTGGGATCCTGTGCCTTTGCAGCCGTTGTGCTGGCACTCGAGTTCCTTCCCTCTTGCCACACCA GGACAGTGTCCCCCCTGCTTCTGCCAGAGCTACTACCCCTTCCTTTTCTTTTGTACAGACCGAGCCCTTC CATCCCCCGGAGCAGGCTTCATCAACGCAGCAGGACCAAGGCCTCCTGAACCGGCCGTCTGCCTTCAGTA CAGTCCAGAGCAGCACTGCGGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGTCGCTCTTTGGG GGGCCCACTGTCCAGCCACCCATCTAGGTATCACAGAGAACTAGCCCCTGGGCTGACAGGTTCTGAGTGG ACCCGGACAGTACTCACTCTGAACTCACGTTCTGAGGTAGAATCTATGCCCCCACCGAGGACCAGTGCCT CCTCAGTGAGTTTGCTTTCTGTACTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTATACTTCAGC CACAGAAGGGAGGGGTTTTCCATCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGTTCCAGCCAA AACAACTCAGGCAACATTCGCCATGAGCTTCAGTGTGACCTGAGACGCTTTTTTTTGGAGTATGACAGGC TGCAGGAGCTGGATCAGAGCCTGAGTGGGGAAACTCCCCAGACCCAACAGGCCCAGGAAATGCTCAACAA TAATATTGAATCTGAGAGACCAGGTCCTTCCCATCAGCCTACCCCACACAGCAGTGAGAACAACTCCAAC CTTTCTCGGGGCCACCTTAATCGATGCCGTGCTTGCCATAATCTTCTAACCTTCAACAATGATACCCTTC GTTGGGAAAGAACCACACCTAACTACTCATCCGGTGAGGCTAGCTCTTCCTGGCATGTCTCCACTACTTT TGAGGGCATGCCACCGAGTGGCAACCAACTACCACCTCTTGAGCGGACTGAGGGCCAAATGCCTAACTCC AGCAGGCTGGAGTTGAGCAGTTCTGCTAGTTCACAAGAGGAGAGGACTGTGGGGGTGGCCTTTAACCAGG AGACCGGCCATTGGGAACGAATTTATACCCAGTCCAGCAGATCTGGAACTGTATCACAGGAGGCCTTACA TCAAGATATGCCTGAGGAAAGCTCTGAGGAAGACTCCCTGAGGAGGAGGCTGCTGGAGTCTTCCCTCATT TCATTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGATTATCTC CTGCTGCATACTACGCCCAGAGGATGATCCAGTATCTCTCAAGGAGAGACAGTATTCGCCAGCGCTCCAT GCGCTACCAACAGAATCGGCTTCGTTCTTCCACCTCCTCTTCTTCCTCAGACAACCAGGGTCCATCAGTA GAGGGAACCGACTTGGAATTTGAGGACTTTGAGGACAATGGTGACAGATCTAGGCACCGAGCTCCCCGAA ATGCCCGAATGTCTGCACCATCACTTGGACGCTTTGTCCCAAGGCGTTTTTTGCTGCCTGAGTACTTGCC TTATGCTGGGATTTTTCATGAGCGTGGACAGCCTGGCTTGGCTACTCATTCTTCTGTTAACAGGGTCCTG GCAGGGGCAGTAATTGGAGATGGACAGTCTGCTGTGGCCAGTAACATTGCCAATACTACCTACAGGCTCC AGTGGTGGGACTTCACCAAGTTTGACCTTCCAGAAATCAGTAATGCCTCCGTGAATGTCCTGGTACAGAA CTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGATGGCCAGCTCCTGGCAGCTTTCATCCCG AGTAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGTATTCCCTGGCCCCCCATAACTTGGGGG AAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCTGTAAGCCTGTCCCCAATGGGCCGATACGT TATGGTGGGCTTGGCCTCACGAAGGATCCTGCTGCACCCCTCTACAGAGCACATGGTAGCCCAGGTCTTC AGGCTGCAGCAGGCCCATGGTGGTGAGACTTCCATGAGGAGAGTTTTCAACGTCCTTTACCCCATGCCTG CGGACCAGCGGAGACACGTCAGCATCAACTCTGCCCGCTGGCTACCTGAGCCAGGGCTCGGTTTGGCCTA TGGCACCAACAAAGGAGACCTGGTGATCTGCCGACCAGAGGCCTTAAACTCTGGCATTGAGTACTACTGG GACCAGCTAAATGAAACGGTCTTCACTGTCCACTCAAGTAGCAGGAGCAGCGAGCGGCCTGGAACCAGCA GAGCCACATGGCGGACAGACAGAGACATGGGCTTGATGAATGCAATTGGGCTGCAACCTCGGAACCCTAC CACCTCTGTGACATCTCAGGGCACCCAGACTCTGGCCCTCCAGTTGCAGAATGCTGAAACACAGACTGAG AGGGAGGAAGAGGAACCAGGGACAGCATCCTCAGGTCCTGGTGAAGGCGAGGGCTCGGAATATGGTGGCA GTGGGGAAGATGCCCTCAGCCGGATCCAGAGGCTAATGGCCGAGGGTGGCATGACAGCTGTGGTACAGCG AGAACAGAGTACCACCATGGCCTCCATGGGTGGCTTCGGCAACAACATCATCGTTAGCCACCGCATTCAC CGCAGCTCCCAGACAGGCACAGAGTCAGGGGCTGCTCGTACCTCCTCACCCCAGCCCTCCACCTCTCGAG GACTACTCTCAGAACCTGGGCAACTGGCAGAGCGAGGACTAAGCCCCCGGACAGCCTCCTGGGACCAGCC TAGTACCTCTGGAAGGGAACTACCCCAGCCAGCCCTGTCCTCTTCCTCCCCTGTCCCCATTCCAGTCCCC CTTGCCAGCAATGAGGGACCAACCATGCACTGCAATGTGACCAATAACAGTCACCTTCCTGAGGGTGATA GTAGCAATGTGGGGGAAGCTGCAGGCCCTAGTGGGGAGCCACGGAACCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206199 representing NM_001134341 Red=Cloning site Green=Tags(s) MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLAS THVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAI ASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDE PEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTE RPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPF HPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEW TRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQ NNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSN LSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNS SRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLI SLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSV EGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVL AGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIP SSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVF RLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYW DQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTE REEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIH RSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVP LASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134341
ORF Size	3900 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134341.1, NP_001127813.1
RefSeq Size	5290 bp
RefSeq ORF	3903 bp
Locus ID	59319
MW	143 kDa

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