Sall1 (NM_001107415) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211859 representing NM_001107415 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGGAGGAAGCAAGCGAAGCCTCAACATTTCCAATCCGACCCCGAAGTGGCCTCGCTCCCCCGGC GAGATGGGGACACAGAGAAGGGTCAACCAAGTCGCCCCACTAAGAGCAAAGATGCCCACGTCTGTGGCCG GTGCTGCGCTGAATTCTTTGAATTATCAGACCTTCTGCTCCACAAAAAGAGCTGCACTAAGAATCAATTA GTTTTGATCGTCAATGAAAGTCCAGCCTCCCCACCAAAAACCTTCCCTCCCGGCCCGCCGTCTCTCAACA ATCCTGATGAGCAAATGAAATTCGTGGCGAGTAAAGCAGACCAAGAAGACTGCGGCGACCTTTCAGAACA CAAGGGACTCGACAGGGAAGAGTCCATGGAGACGGAGGTCCCAGTTGCCACCAGCAGCAGCAGCAGCAAC GGTGGCACTCTGAGCAGTGTCACCAACATCGCCACCCCAAGCTGCCACAGCGGCTCTTCCACGGGTACCT CAGCGATCACAACCTCTCTACCTCAACTTGGGGACCTGACGACACTGGGCAACTTCTCGGTGATCAACAG CAACGTCATCATTGAAAATCTCCAGAGCACCAAGGTGGCGGTGGCCCAGTTCTCCCAGGAAGCGAGGTGT GGGGGGGCCTCTGGAGGCAAGCTGCTCATCTCGGCCCTCATGGAGCAGCTCCTAGCTCTGCAGCAGCAGC AGATCCACCAGCTGCAGCTCATAGAACAGATTCGTCACCAAATACTGCTGCTGGCTTCTCAGAACGCAGA TCTGTCAGCCTCTTCTGTTCCTTCTCAAGGTACTTTACGAACGTCTGCCAACCCCTTGACCACACTAAGT TCCCATTTGTCTCAGCAGCTGGCGGTGGCAGCTGGATTAGCACAGAGCCTTGCTAGCCAATCTGCCAACA TCAGCGGTGTGAAGCAGCTCCCCCCAGTCCAGCTACCTCAGAGCAGCTCCGGCACCATCATCCCGCCCAG TAGTGGCCCATCTCCCAACGTGAGCATGGTGACAGCAACAGTTCCCACCCCGTCCTCGGAAAAAGTGGCT TCCAATGCTGGTGCCTCCCATGTCAGCCCCCCGGCCGTCTCAGCATCATCCTCACCAGCAGCATTCGCAA TAAGCAGTCTGTTGAGTCCTGCATCTAACCCACTTCTACCTCAGCCGACCCCAGCTAATGCTGTTTTCCC TACCCCTTTGCCTAACATTGCGACAACTGCAGAAGATTTAAACGCCCTGTCTGCCTTGGCCCAGCAAAGA AAAAGCAAGCCACCAAATGTCACTGCCTTCGAAGCAAAGAGTACTTCAGACGAGGCATTCTTTAAACACA AGTGTAGGTTCTGTGCAAAGGTCTTTGGGAGTGACAGTGCCTTGCAGATCCACTTGCGCTCCCATACTGG AGAGAGGCCGTTCAAGTGCAACATCTGTGGGAACAGGTTCTCCACCAAGGGCAACCTGAAAGTCCACTTT CAGCGCCACAAAGAGAAGTACCCCCACATCCAGATGAACCCCTACCCCGTACCGGAGCACTTGGACAACG TCCCTACGAGCACCGGCATCCCCTACGGCATGTCCATCCCTCCAGAGAAGCCGGTCACCAGCTGGCTAGA CACCAAGCCGGTCCTGCCCACTCTGACCACTTCCGTCGGCCTGCCATTGCCTCCTACCCTCCCAAGCCTC ACGCCCTTCATCAAGACGGAAGAACCAGCTCCCATCCCAATTAGCCATTCTGCTGCCAGCCCCCAAGGCT CAGTCAAAAGTGACTCGGGGGCCCCCGATTTGGCCACAAGAAACCCAAGTGGGGTCCCAGAGGAAGCCGA AGGGTCTGCTGTGCCCCCCTTCGGTGGCAAGGGTGAAGACAGTAGCGTGGTAAGCTCCATCCCGACAGCG GGCAGCAGTGCCCTGAACTCCCCAGTGGCTGATGGTGGTGCAGGAGGGACCACCTTCACCAACCCTCTGT TGCCCCTCATGTCTGAGCAGTTCAAGGCCAAGTTTCCTTTTGGGGGACTCTTAGATTCAGCCCAGGCCTC AGAGACATCCAAGCTGCAGCAACTGGTAGAAAACATTGACAAGAAGGCCACCGACCCCAACGAGTGTGTC ATCTGCCATCGGGTTCTGAGCTGTCAGAGCGCTTTGAAAATGCACTACCGAACACACACCGGGGAGAGGC CCTTCAAGTGTAAGATCTGCGGCCGGGCTTTTACCACGAAAGGGAACCTCAAGACCCACTACAGCGTCCA TCGGGCTATGCCCCCACTCAGAGTCCAGCATTCCTGCCCCATCTGTCAGAAGAAGTTCACAAACGCAGTG GTCCTCCAGCAGCACATCCGTATGCACATGGGAGGCCAGATCCCGAACACCCCGGTCCCTGACAACTACC CCGAGTCCATGGAGTCTGACACAGGCTCCTTCGATGAGAAGAATTTCGATGACTTAGACAACTTCTCAGA TGAGAACATGGAAGAGTGTCCTGAGGGCAGCATCCCGGATACGCCCAAGTCAGCTGACGCTTCCCAAGAC AGCCTGTCGTCTTCACCTCTGCCACTCGAGATGTCCAGCATCGCCGCTTTGGAAAATCAGATGAAGATGA TCAACGCTGGCCTGGCCGAGCAGCTGCAGGCCAGCCTGAAGTCGGTGGAGAACGGGTCAGTGGAAGGAGA TGTACTGACCAATGACTCATCCTCAGTGGGTGGGGATGTGGAGAGCCAGAGTTCGGGCAGCCCAGCCATC TCAGAGTCTACCTCTTCTATGCAGGCTCTGTCCCCATCCAACAGCACCCAAGAGTTCCACAAGTCACCCA ATGTTGAGGAAAAGCCACAGAGAGTGGGGCCAGGCGAGTTTGCCAATGGCCTGTCTCCCACCCCAGTGAA CGGAGGTGCTTTGGACTTGACCTCTAGTCATGCAGAGAAAATCATCAAAGAAGATTCCCTGGGAATCCTC TTCCCTTTCAGAGACCGGGGTAAATTTAAAAACACTGCTTGTGACATTTGTGGCAAAACCTTTGCTTGTC AGAGTGCCTTGGACATTCATTACAGAAGTCATACCAAAGAGAGACCGTTCATTTGCACAGTTTGCAGTCG TGGCTTTTCCACAAAGGGGAATTTGAAGCAGCACATGTTGACACATCAGATGCGAGATCTGCCATCGCAG CTCTTTGAGCCCAGTTCCAACCTCGGCCCCAATCAGAACTCTGCGATGATTCCCGCCAGCTCGTTGTCAT CTCTCATCAAAACAGAGGTCAACGGCTTTGTGCACGTTTCTCCTCAGGACAGTAAGGATGCCCCCACTAG TCATGTCCCTTCGGGGCCTCTGTCATCCTCTGCGACATCCCCGGTTCTGCTCCCAGCTCTGCCCCGGAGA ACCCCCAAACAGCACTATTGTAACACATGTGGTAAAACCTTCTCTTCCTCGAGTGCCCTGCAGATCCACG AGAGAACTCACACTGGAGAGAAGCCCTTTGCTTGCACTATCTGTGGAAGAGCATTCACAACGAAAGGCAA TCTGAAGGTCCACATGGGCACTCATATGTGGAACAGTACCCCTGCACGCCGGGGCCGCCGGCTCTCAGTG GATGGCCCCATGACATTTCTAGGAGGGAATCCTGTCAAGTTCCCAGAAATGTTCCAGAAGGATCTGGCGG CGAGGTCAGGAAGCGGGGATCCGTCCAGCTTTTGGAACCAGTATACAGCAGCACTGTCCAACGGGCTGGC CATGAAGGCCAACGAGATCTCTGTCATTCAGAATGGTGGCATCCCTCCAATTCCTGGAAGCCTGGGCAAC GGGAGCAGCTCACCTATCAGCGGGCTGACAGGGAACGTGGAAAAGCTGGGGAATTCGGAACCCAGTGCTC CCCTGGCTGGTCTGGAGAAAATGGCGAGCAGCGAGAACGGAACCAACTTCCGTTTCACCCGCTTCGTGGA GGACAGCAAAGAGATCGTCACGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211859 representing NM_001107415 Red=Cloning site Green=Tags(s) MSRRKQAKPQHFQSDPEVASLPRRDGDTEKGQPSRPTKSKDAHVCGRCCAEFFELSDLLLHKKSCTKNQL VLIVNESPASPPKTFPPGPPSLNNPDEQMKFVASKADQEDCGDLSEHKGLDREESMETEVPVATSSSSSN GGTLSSVTNIATPSCHSGSSTGTSAITTSLPQLGDLTTLGNFSVINSNVIIENLQSTKVAVAQFSQEARC GGASGGKLLISALMEQLLALQQQQIHQLQLIEQIRHQILLLASQNADLSASSVPSQGTLRTSANPLTTLS SHLSQQLAVAAGLAQSLASQSANISGVKQLPPVQLPQSSSGTIIPPSSGPSPNVSMVTATVPTPSSEKVA SNAGASHVSPPAVSASSSPAAFAISSLLSPASNPLLPQPTPANAVFPTPLPNIATTAEDLNALSALAQQR KSKPPNVTAFEAKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHF QRHKEKYPHIQMNPYPVPEHLDNVPTSTGIPYGMSIPPEKPVTSWLDTKPVLPTLTTSVGLPLPPTLPSL TPFIKTEEPAPIPISHSAASPQGSVKSDSGAPDLATRNPSGVPEEAEGSAVPPFGGKGEDSSVVSSIPTA GSSALNSPVADGGAGGTTFTNPLLPLMSEQFKAKFPFGGLLDSAQASETSKLQQLVENIDKKATDPNECV ICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAV VLQQHIRMHMGGQIPNTPVPDNYPESMESDTGSFDEKNFDDLDNFSDENMEECPEGSIPDTPKSADASQD SLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSVEGDVLTNDSSSVGGDVESQSSGSPAI SESTSSMQALSPSNSTQEFHKSPNVEEKPQRVGPGEFANGLSPTPVNGGALDLTSSHAEKIIKEDSLGIL FPFRDRGKFKNTACDICGKTFACQSALDIHYRSHTKERPFICTVCSRGFSTKGNLKQHMLTHQMRDLPSQ LFEPSSNLGPNQNSAMIPASSLSSLIKTEVNGFVHVSPQDSKDAPTSHVPSGPLSSSATSPVLLPALPRR TPKQHYCNTCGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSV DGPMTFLGGNPVKFPEMFQKDLAARSGSGDPSSFWNQYTAALSNGLAMKANEISVIQNGGIPPIPGSLGN GSSSPISGLTGNVEKLGNSEPSAPLAGLEKMASSENGTNFRFTRFVEDSKEIVTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107415
ORF Size	3945 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107415.2, NP_001100885.2
RefSeq Size	5124 bp
RefSeq ORF	3948 bp
Locus ID	307740
MW	139.5 kDa

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