Brca1 (NM_012514) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212756 representing NM_012514 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTATCTGCTGTTCGAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTGGAGT GTCCAATCTGTTTGGAACTGATCAAAGAACCGGTTTCCACACAGTGCGACCACATATTTTGCAAATTTTG TATGCTGAAACTCCTTAACCAGAAGAAAGGACCTTCCCAGTGTCCTTTGTGTAAGAATGAGATAACCAAA AGGAGCCTACAAGGAAGTGCAAGGTTTAGTCAACTTGTTGAAGAGCTGCTGAAAATCATTGATGCTTTTG AGCTTGACACCGGAATGCAGTGTGCAAATGGTTTTAGTTTTTCAAAAAAGAAAAATAGTTCTTCTGAGCT TCTGAATGAGGACGCTTCTATCATCCAGAGTGTGGGCTACCGAAACCGCGTCAAAAAACTTCAACAGATT GAGTCTGGAAGTGCCACCTTGAAGGACAGTCTCAGTGTCCAGTTGTCTAACCTTGGGATTGTGAGATCAA TGAAGAAAAATAGGCAGACACAACCTCAAAACAAATCTGTCTACATTGCTTTAGAATCTGATTCTTCTGA GGAGAGAGTAAATGCACCCGATGGCTGCAGTGTGAGAGATCAGGAATTGTTTCAGATCGCCCCTGGAGGA GCTGGAGATGAAGGCAAGTTGAACTCTGCAAAAAAGGCTGCTTGTGACTTTTCTGAGGGCATAAGAAACA TTGAACATCATCAATGCAGTGATAAAGATTTGAACCCCACTGAGAATCATGCAACTGAAAGGCATCCAGA AAAATGTCCGCGTATTTCTGTTGCAAACGTGCACGTGGAGCCGTGTGGCACAGATGCTCGTGCCAGCTCA TTACAGCGTGGGACCCGCAGTTTATTGTTCACTGAGGACAGACTGGATGCAGAAAAGGCTGAATTCTGTG ATAGAAGCAAACAGTCTGGCGCAGCAGTGAGCCAGCAGAGCAGATGGGCTGACAGTAAAGAAACATGTAA TGGCAGGCCGGTTCCCCGCACTGAGGGAAAGGCAGATCCAAATGTGGATTCCCTCTGTGGTAGAAAGCAG TGGAATCATCCGAAAAGCCTGTGCCCTGAGAATTCTGGAGCTACCACTGACGTTCCTTGGATAACACTGA ATAGCAGCATTCAGAAAGTGAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAATGCATC TGACAGGAGGCCTGCGTCAAATGCAGAAGCTGCTGTTGTGTTAGAAGTTTCAAATGAAGTGGATGGATGT TTCAGTTCTTCAAAGAAAATAGACTTAGTTGCCCCTGATCCCGATAATGCTGTAATGTGTACAAGTGGAA GAGACTTCTCCAAGCCAGTAGAGAATATTATCAACGATAAAATATTTGGGAAAACCTATCAGAGAAAGGG AAGCCGCCCTCACTTGAACCATGTGACTGAAATTATAGGCACATTTACTACAGAACCACAGATTATACAA GAGCAGCCCTTCACAAATAAATTAAAACGCAAAAGAAGTACATGCCTTCATCCTGAGGACTTTATCAAGA AAGCAGATTTAACAGTTGTTCAAAGGATTTCTGAAAATTTAAATCAGGGAACTGACCAAATGGAGCCAAA TGACCAAGCAATGAGTATTACCAGTAACGGTCAGGAGAACAGAGCAACAGGTAATGATCTTCAGAGAGGG AGAAATGCTCATCCAATAGAATCATTGAGAAAGGAACCTGCTTTCACAGCTAAAGCCAAATCTATAAGCA ACAGTATAAGTGATTTGGAGGTAGAATTAAATGTTCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAG GAGGAAGTCTACCAGGTGTGTTCTTCCACTCGAACCAATCAGTAGAAATCCGAGCCCACCTACTTGTGCT GAACTTCAGATCGAGAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACAATTCCAACCAAACCCCAGCCG GGCACATTAGAGAGCCTCAACTCATCGAAGACACAGAACCCGCAGCTGATGCCAAGAAGAACGAGCCAAA TGAACACATAAGGAAGAGAAGTGCCAGTGATGCGTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTA TTAACTAGCTGTTCAAGTCCTAGAAAGCCTCAAGGACCTGTCAATCCTAGCCCTGAGAGAAAAGGAATAG AGCAACTTGAAATGTGCCAGATGCCTGATAATAACAAAGAACTCGGGGATTTGGTCCTGGGAGGAGAGCC CAGTGGGAAACCTACTGAACCATCTGAGGAGAGCACCAGTGTGTCCTTGGTACCCGACACAGACTACGAC ACCCAGAACAGTGTCTCAATACTGGAAGCGAACACTGTCAGATATGCAAGAACAGGATCAGTTCAGTGTA TGACTCAGTTTGTCGCAAGTGAAAACCCCAAGGAACTTGTCCATGGTTCTAACAATGCTGGAAGTGGCTC GGAGTGCTTCAAGCACCCATTGAGACATGAACTTAACCACAATCAAGAGACAATAGAAATGGAAGACAGT GAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAA AACTTAGAAGTCCCCAAAAGGACTGTACTCTGGTAGGTGCCCGCTCTGTGCCCTCAAGGGAACCAAGTCC AAAGGTGACTTCTAGAGGTGAACAAAAAGAACGTCAGGGACAAGAAGAGTCTGAAATCAGTCATGTACAG GCAGTCACAGTCACAGTAGGCTTACCTGTGCCCTGTCAGGAAGGTAAGCCAGGTGCTGTTACAATGTGTG CTGATGTTTCTAGGCTTTGTCCGTCATCTCATTATAGAAGCTGTGAGAATGGACTCAACACCACAGATAA ATCTGGAATTTCACAAAACTCACATTTTAGACAATCAGTTTCTCCCCTCAGGTCATCTATAAAAACTGAC AATAGAAAAACTCTGACAGAGGGNCGATTTGAGAAACATACTGAAAGGGGRATGGGAAATGAGACTGCTG TTCAAAGTACAATACACACAATTAGTCTAAATAACAGAGGAGATGCTTGTCTAGAAGCCAGCTCAGGCAG TGTTATTGAAGTACATTCCACTGGTGAAAACGTCCAGGGGCAACTAGATAGAAACAGAGGGCCTAAGGTA AACACCGTGTCTCTATTAGATAGTACACAGCCTGGTGTCTCTAAGCAGAGTGCTCCTGTAAGTGATAAGT ATCTTGAAATAAAGCAGGAGAGTAAGGCTGTCAGTGCAGACTTCTCTCCATGTCTGTTCTCAGATCATCT TGAAAAACCTATGAGAAGTGATAAGACTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGTTGGATGAT GTTGAAATACAGGAAAATGCTAGCTTCGGTGAAGGTGGCATAACGGAAAAGTCTGCTATTTTTAATGGAA GTGTCCTGAGAAGAGAGTCCAGTAGGAGCCCTAGCCCTGTAACCCATGCATCGAAGTCGCGGAGTCTCCA CAGAGGGTCTAGGAAATTAGAATTCTCAGAAGAGAGCGACTCCACTGAGGATGAAGACCTTCCCTGCTTC CAACACTTGCTGAGCAGAGTAAGCAGCACACCCGAACTTACCAGATGCAGCAGTGTGGTGACACAGCGTG TGCCAGAGAAAGCGAAGGGGACCCAAGCACCCCGGAAGAGTAGCATCAGTGACTGCAATAATGAGGTGAT CTTGGGAGAGGCATCTCAGGAATATCAGTTTAGTGAGGATGCAAAATGCTCTGGCAGCATGTTCTCCTCA CAGCACAGTGCTGCCCTAGGTTCNCCTGCAAATGCACTCTCCCAGGATCCCGATTTTAATCCACCTTCCA AACAAAGGCGTCACCAGGCTGAGAATGAGGAAGCCTTTCTAAGTGATAAGGAATTGATTTCAGATCATGA GGACATGGCAGCTTGCCTGGAAGAGGCTAGCGATCAAGAAGAGGACAGTATAATCCCGGACTCAGTGGCG TCTGGATATGAGAGTGAAGCAAACCTCTCTGAAGACTGCTCACAGAGTGATATTCTAACCACTCAGCAGA GGGCGACCATGAAGGATAACCTGATAAAGCTCCAGCAGGAAATGGCTCAGCTGGAAGCTGTGCTAGAGCA GCATGGGAGCCAGCCGTCTGGCCACCCCCCTTGCCTCCCAGCTGACCCTTGTGCCCTAGAAGACCTGCCA GATCCAGAACAAAACAGATCAGGAACAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCC AGAATCCAAAGCGCGCCTGTGATGACAAATCCCAACCACAACCTCCGGACGGTCTCCCCAGTGGAGATAA GGAGTCAGGAATGAGAAGGCCTTCCCCTTTTAAATCTCCACTGACCAGCAGTAGGTGCTCTGCACGAGGC CACTCCAGGAGTCTTCAGAATAGAAACTCCACSTCGCAAGAGGAGCTCCTTCAGCCTGCTGRATTGGAGA AGTCATGTGAACCACACAACTTAACAGGGCGGTCTTGCTTGCCAAGGCAAGATCTCGAAGGAACCCCATA CCCGGAATCTGGAATCAGGCTAGTCTCCAGCAGAGACCCTGATTCTGAATCCCCTAAAGTGTCAGCCCTT GTTTGCACTGCACCAGCTTCAACCTCTGCACTGAAAATATCCCAAGGTCAAGTTGCTGGGTCTTGCAGGA GTCCAGCTGCTGGTGGTGCTGATACTGCAGTGGTAGAAATTGTGAGCAAGATAAAGCCGGAAGTGACGTC TCCAAAAGAACGAGCGGAGAGAGATATATCCATGGTGGTGTCAGGCTTGACCCCTAAAGAAGTAATGATT GTCCAAAAGTTTGCTGAAAAATACCGCCTTGCTTTAACTGATGTAATTACTGAGGAAACTACGCATGTAA TTATAAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAA GTGGATAGTTAGCTATTCTTGGGTGATCAAGTCTATCCAAGAAAGAAAACTTCTGAGTGTGCATGAATTT GAAGTCAAAGGAGACGTTGTGACTGGAAGCAACCACCAAGGTCCAAGGCGATCCAGAGAATCCCAGGAAA AGCTCTTTGAAGGCCTACAGATCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAG GATGCTGCAGCTGTGTGGGGCTTCTGTGGTGAAGGAGCTTCCATTGCTCACCCGTGACACAGGTGCTCAT CCAATTGTGCTCGTGCAGCCAAGTGCCTGGACAGAAGACAACGACTGCCCTGATATTGGGCAGCTGTGCA AGGGACGTCTAGTGATGTGGGACTGGGTGTTGGACAGTATATCCGTCTACCGGTGTCGGGATCTGGATGC CTACCTGGTACAGAATATCACCTGTGGCCGTGATGGTAGCGAGCCACAAGACTCCAATGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212756 representing NM_012514 Red=Cloning site Green=Tags(s) MDLSAVRIQEVQNVLHAMQKILECPICLELIKEPVSTQCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITK RSLQGSARFSQLVEELLKIIDAFELDTGMQCANGFSFSKKKNSSSELLNEDASIIQSVGYRNRVKKLQQI ESGSATLKDSLSVQLSNLGIVRSMKKNRQTQPQNKSVYIALESDSSEERVNAPDGCSVRDQELFQIAPGG AGDEGKLNSAKKAACDFSEGIRNIEHHQCSDKDLNPTENHATERHPEKCPRISVANVHVEPCGTDARASS LQRGTRSLLFTEDRLDAEKAEFCDRSKQSGAAVSQQSRWADSKETCNGRPVPRTEGKADPNVDSLCGRKQ WNHPKSLCPENSGATTDVPWITLNSSIQKVNEWFSRTGEMLTSDNASDRRPASNAEAAVVLEVSNEVDGC FSSSKKIDLVAPDPDNAVMCTSGRDFSKPVENIINDKIFGKTYQRKGSRPHLNHVTEIIGTFTTEPQIIQ EQPFTNKLKRKRSTCLHPEDFIKKADLTVVQRISENLNQGTDQMEPNDQAMSITSNGQENRATGNDLQRG RNAHPIESLRKEPAFTAKAKSISNSISDLEVELNVHSSKAPKKNRLRRKSTRCVLPLEPISRNPSPPTCA ELQIESCGSSEETKKNNSNQTPAGHIREPQLIEDTEPAADAKKNEPNEHIRKRSASDAFPEEKLMNKAGL LTSCSSPRKPQGPVNPSPERKGIEQLEMCQMPDNNKELGDLVLGGEPSGKPTEPSEESTSVSLVPDTDYD TQNSVSILEANTVRYARTGSVQCMTQFVASENPKELVHGSNNAGSGSECFKHPLRHELNHNQETIEMEDS ELDTQYLQNTFQVSKRQSFALFSKLRSPQKDCTLVGARSVPSREPSPKVTSRGEQKERQGQEESEISHVQ AVTVTVGLPVPCQEGKPGAVTMCADVSRLCPSSHYRSCENGLNTTDKSGISQNSHFRQSVSPLRSSIKTD NRKTLTEXRFEKHTERXMGNETAVQSTIHTISLNNRGDACLEASSGSVIEVHSTGENVQGQLDRNRGPKV NTVSLLDSTQPGVSKQSAPVSDKYLEIKQESKAVSADFSPCLFSDHLEKPMRSDKTFQVCSETPDDLLDD VEIQENASFGEGGITEKSAIFNGSVLRRESSRSPSPVTHASKSRSLHRGSRKLEFSEESDSTEDEDLPCF QHLLSRVSSTPELTRCSSVVTQRVPEKAKGTQAPRKSSISDCNNEVILGEASQEYQFSEDAKCSGSMFSS QHSAALGXPANALSQDPDFNPPSKQRRHQAENEEAFLSDKELISDHEDMAACLEEASDQEEDSIIPDSVA SGYESEANLSEDCSQSDILTTQQRATMKDNLIKLQQEMAQLEAVLEQHGSQPSGHPPCLPADPCALEDLP DPEQNRSGTAILTSKNINENPVSQNPKRACDDKSQPQPPDGLPSGDKESGMRRPSPFKSPLTSSRCSARG HSRSLQNRNSXSQEELLQPAXLEKSCEPHNLTGRSCLPRQDLEGTPYPESGIRLVSSRDPDSESPKVSAL VCTAPASTSALKISQGQVAGSCRSPAAGGADTAVVEIVSKIKPEVTSPKERAERDISMVVSGLTPKEVMI VQKFAEKYRLALTDVITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVIKSIQERKLLSVHEF EVKGDVVTGSNHQGPRRSRESQEKLFEGLQIYCCEPFTNMPKDELERMLQLCGASVVKELPLLTRDTGAH PIVLVQPSAWTEDNDCPDIGQLCKGRLVMWDWVLDSISVYRCRDLDAYLVQNITCGRDGSEPQDSND TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012514
ORF Size	5451 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012514.1, NP_036646.1
RefSeq Size	5607 bp
RefSeq ORF	5454 bp
Locus ID	497672
UniProt ID	O54952
MW	200.1 kDa
Gene Summary	involved in the DNA damage response and DNA repair [RGD, Feb 2006]

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