Pde4dip (NM_022382) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214670 representing NM_022382 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTAATGGATATCGCACTCTGTCCCAGCACCTCAATGACCTGAAGAAGGAGAACTTCAGCCTCAAGC TGCGCATCTACTTCCTGGAGGAGCGCATGCAACAGAAGTATGAAGTCAGCCGGGAGGACGTCTACAAGCG GAACATTGAGCTGAAGGTTGAAGTGGAGAGCCTGAAACGAGAGCTCCAGGACAGGAAACAGCATCTACAT AAAACATGGGCCGATGAGGAGGATCTCAACAGCCAGAATGAAGCAGAGCTCCGGCGCCAGGTTGAAGAAC CGCAGCAGGAGACAGAACACGTTTATGAGCTCCTAGACAACAACATTCAGCTGCTGCAGGAGGAATCCAG GTTTGCAAAGGATGAAGCCACACAGATGGAGACTCTGGTGGAGGCAGAGAAGGGGTGTAATCTGGAGCTC TCAGAGAGGTGGAAGGATGCTACCAAGAACAGGGAAGATGCACCGGGAGACCAGGTGAAGCTTGACCAAT ATTCTGCGGCACTGGCTCAGAGGGACAGGAGAATTGAAGAGCTGAGGCAGAGCTTGGCTGCCCAGGAGGG GCTTGTGGAACAGCTGTCTCGAGAGAAACAACAACTGTTACATCTGCTGGAGGAGCCTGGGGGCATGGAA GTGCAGCCCATGCCTAAAGGGTTACCCACGCAACAAAAGCCAGACCTAAATGAGACCCCTACAACCCAGC CATCTGTGTCTGATTCCCACCTGGCAGAACTCCAGGACAAAATCCAGCAAACAGAGGTCACCAACAAGAT TCTTCAAGAGAAACTGAATGACATGAGCTGTGAGCTCAGATCTGCACAGGAGTCGTCTCAGAAGCAAGAT ACGACAATCCAAAGCCTCAAGGAAATGCTAAAGAGCAGGGAAAGTGAGACTGAAGAGCTGTACCAGGTGA TTGAAGGTCAAAATGACACAATGGCAAAGCTTCCGGAAATGCTACACCAGAGCCAGCTCGGACAGCTCCA GAGCTCAGAGGGCATTGCCCCTGCTCAGCAGCAAGTGGCCCTGCTTGACCTTCAGAGTGCTCTGTTCTGC AGCCAGCTTGAAATCCAGAAGCTCCAGAGGCTGTTACGCCAGAAAGAGCGTCAGCTGGCTGACGGCAAGC GGTGCATGCAATTTGTGGAGGCTGCAGCACAGGAGAGAGAGCAGCAGAAGGAAGCTGCTTGGAAACATAA CCAGGAATTACGAAAAGCTTTGCAACACCTCCAAGGAGAACTGCACAGTAAGAGCCAACAGCTCCACGTT CTGGAGGCAGAAAAATATAATGAAATTCGAACCCAGGGACAAAACATTCAACACCTAAGTCACAGTCTGA GTCACAAAGAGCAGCTAATTCAGGAACTTCAGGAGCTCCTACAGTATCGGGATACCACAGACAAAACTCT AGACACAAATGAGGTGTTTCTTGAGAAACTACGGCAACGAATACAAGACCGGGCAGTTGCTCTAGAGCGG GTTATAGATGAAAAGTTCTCTGCTCTAGAAGAAAAGGACAAGGAACTGCGGCAGCTCCGGCTTGCTGTGA GGGACCGAGACCATGACTTAGAGAGACTGCGTTGTGTCCTGTCTGCCAATGAAGCTACCATGCAAAGTAT GGAGAGTCTCCTGAGGGCCAGAGGCCTGGAAGTGGAGCAGTTAATTGCCACCTGCCAAAACCTCCAGTGG TTGAAGGAAGAATTGGAAACCAAGTTTGGCCACTGGCAGAAGGAACAGGAGAGCATCATTCAGCAGTTAC AGACATCTCTGCATGACAGGAACAAAGAAGTAGAGGATCTCAGTGCAACTTTGCTCCACAAACTTGGACC CGGCCAGAGTGAAGTAGCTGAGGAGCTGTGCCAGCGCCTGCAGCGGAAGGAAAGGGTGCTGCAGGACCTT CTGAGTGATCGGAACAAACAAGCCATGGAGCACGAGATGGAGGTCCAGGGACTGCTCCAGTCGATGGGCA CCCGGGAACAGGAAAGACAGGCTGTTGCAGAAAAAATGGTACAAGCCTTCATGGAAAGAAACTCGGAATT ACAGGCCCTGCGGCAGTATCTAGGGGGGAAGGAATTAATGGCAGCATCTCAGGCATTCATCTCTAACCAA CCAGCTGGAGCGACTTCTGTAGGCCCCCACCATGGAGAGCAAACTGACCAAGGTTCTACGCAGATGCCCT CTCGAGACGACAGCACCTCGCTGACTGCCAGAGAGGAGGCCAGCATACCCCGGTCTACATTAGGAGACTC AGACACAGTTGCAGGGCTGGAGAAAGAACTGAGCAATGCCAAGGAGGAGCTTGAGCTCATGGCCAAAAAA GAAAGAGAAAGCCAGATAGAATTGTCTGCCCTGCAGTCCATGATGGCTGTGCAAGAGGAAGAGCTGCAGG TGCAGGCTGCTGACTTGGAGTCCCTGACCAGGAACATACAGATAAAAGAAGACCTCATAAAGGACCTGCA AATGCAACTGGTTGACCCTGAAGATATGCCAGCCATGGAGCGCCTGACCCAAGAGGTCTTACTTCTTCGG GAAAAAGTTGCTTCAGTGGAACCCCAGGGTCAGGAAGGGTCAGAGAACAGGAGACAACAGTTGCTGCTGA TGTTAGAAGGACTAGTGGATGAACGGAGTCGGCTCAACGAGGCCCTGCAAGCTGAGCGGCAGCTCTACAG CAGCCTGGTCAAGTTCCATGCCCAACCAGAGACTTTTGAGAGAGACCGAACTCTGCAGGTGGAACTGGAA GGGGCCCAGGTGTTACGCAGTCGACTAGAAGAAGTTCTTGGAAGAAGCCTGGAGCGCTTAAGCAGGCTGG AGACCCTGGCCGCCATTGGAGGTGCTACTGCAGGCGATGAGACTGAAGATACAAGCACACAGTTCACAGA CAGCATTGAGGAGGAGGCTGCACACAACAGCCACCAGCAACTCATCAAGGTGTCTTTGGAGAAAAGCCTG ACCACCATGGAGACCCAGAACACATGTCTTCAGCCCCCTTCCCCAGTAGGAGAGGATGGTAACAGGCATC TTCAGGAAGAAATGCTCCACCTGAGGGCTGAAATCCACCAGCCCTTAGAAGAGAAGAGAAAAGCTGAGGC AGAACTCAAGGAGCTAAAGGCTCAAATTGAGGAAGCAGGATTCTCCTCTGTGTCCCACATCAGGAACACC ATGCTGAGCCTTTGCCTTGAGAATGCAGAGCTGAAAGAGCAGATGGGAGAAGCAATGTCTGATGGATGGG AGGTGGAGGAAGACAAGGAGAAGGGCGAGGTGATGGTGGAGACCGTGGTGGCCAAAGGGGGTCTGAGTGA GGACAGCCTTCAGGCTGAGTTCAGGAAAGTCCAGGGGAGACTCAAGAGTGCCTACAACATCATCAACCTC CTCAAAGAGCAGCTGGTCCTGAGAAGCTCGGAAGGGAACACTAAGGAGATGCCAGAGTTCCTCGTGCGCC TGGCCAGGGAGGTGGACAGAATGAACATGGGCTTGCCTTCCTCGGAGAAGCATCAACACCAAGAACAGGA GAATATGACCGCAAGGCCTGGCCCCAGGCCCCAGAGTCTCAAGCTTGGGACAGCTCTCTCAGTAGACGGC TACCAACTGGAGAACAAGTCCCAGGCCCAAGACTCTGGACATCAGCCAGAATTTAGCCTACCAGGGTCCA CCAAACACCTGCGCTCCCAGCTGGCTCAGTGTAGACAACGGTACCAAGATCTCCAGGAGAAGCTGCTCAT CTCAGAAGCCACTGTGTTTGCCCAGGCAAACCAGCTAGAGAAGTACAGAGCCATATTAAGTGAATCCCTG GTGAAGCAGGACAGCAAGCAGATCCAGGTGGACCTTCAGGACCTGGGCTATGAGACTTGTGGCCGAAGTG AGAATGAAGCTGAACGTGAGGAGACCACCAGCCCTGAGTGTGAGGAGCACGGTAACCTGAAGCCTGTGGT GCTGGTGGAAGGCTTGTGCTCTGAGCAAGGGTACCTGGACCCTGTCTTGGTCAGCTCACCTGTGAAGAAC CCTTGGAGAACAAGCCAGGAAGCGAGAGGAGTCCAGGCACAAGGAACTTCAGACGACAGCTCTCTCCTGA GGAAGGACATCCGAGATCTGAAAGCCCAGCTACAGAATGCCTACAAGGTCATTCAGAACCTGAGGAGCCG GGTCCGGTCCCTGTCTGCCACAAGCGATTACTCATCGAGTCTGGAGAGACCCCGCAAGCTGAGAGCCGTG GCAACCCTTGAGGGGGCCTCACCCCACAGTGTCACTGATGAAGACGAGGGCTTGTTGTCAGATGGCACCG GGGCTTTTTACCCTCCAGGGCTCCAGGCCAAAAAGAATCTAGAGAATCTCATCCAGAGAGTATCCCAGCT GGAGGCCCAGCTCCCCAAAACTGGACTAGAAGGGAAGCTGGCTGAAGAGCTGAAGTCCGCCTCGTGGCCT GGAAAATACGATTCTTTGATTCAGGATCAGGCCCGAAAAACTGTCATATCTGCGTCCGAAAATACCAAAA GGGAGAAGGATTTGTTTTCTTCTCACCCAACATTCGAAAGATACGTCAAATCTTTTGAAGACCTCCTGAG GAACAACGACTTGACTACTTACCTGGGCCAGAGCTTCCGGGAACAACTTAGTTCAAGGCGTTCAGTGACA GACAGGCTGACCAGCAAATTCAGCACAAAGGATCATAAGAGTGAAAAAGAAGAAGCTGGGCTTGAGCCAC TGGCCCTCAGGCTCAGCAGGGAACTACAGGAGAAAGAGAAAGTGATTGAAGTCCTGCAGGCCAAGCTGGA TACCCGGTTCTCCTCACCCCCCAGCAGCCATGCTGCGTCTGACTCCCACCGCTCTGCCAGCAGCACATCT TTCCTGTCGGATGACATAGAAGCCTGCTCTGACATGGACGTAGCCAGCGAGTACACACACTATGAAGAGA AGAAGCCCTCACCCAGTAACTCAGCAGCCAGTGCATCTCAGGGGCTTAAGGGCGAGCCCAGAAGCAGCTC CATCAGCTTGCCAACTCCCCAGAACCCCCCTAAGGAGGCCAGCCAGGCCCAGCCAGGCTTTCACTTTAAC TCCATACCCAAGCCGGCTAGCCTTTCCCAGGCACCAATGCACTTCACTGTACCCAGCTTCATGCCTTTCG GCCCCTCTGGGCCTCCCCTTCTTGGTTGCTGTGAGACACCAGTGGTGTCCTTGGCTGAGGCTCAACAAGA GCTGCAGATGCTGCAGAAGCAGCTGGGACGAAGTGTTAGCATTGCCCCTCCCACCTCCACATCCACGTTG CTTAGCAACCACACAGAAGCTAGCTCTCCCCGCTACAGCAACCCTGCTCAGCCCCACTCCCCAGCAAGGG GCACCATAGAGCTGGGCAGAATCCTGGAGCCTGGATACCTGGGCAGCGGCCAGTGGGACATGATGAGGCC TCAGAAAGGGAGCATCTCTGGGGAGCTGTCCTCAGGCTCCTCGATGTACCAGCTTAACTCCAAACCCACA GGGGCCGACCTGTTGGAAGAGCATTTAGGTGAGATCCGGAACCTGCGCCAGCGCCTGGAGGAGTCCATAT GTGTCAATGACAGGCTACGGGAGCAGCTGCAGCATAGGCTCAGCTCCACGGCCCGAGAAAATGGTTCCAC CTCTCACTTCTACAGTCAGGGCCTGGAGTCCATGCCTCAGCTCTACAATGAGAACAGAGCCCTCAGGGAA GAAAACCAAAGCCTGCAGACACGGCTCAGTCATGCTTCCAGGGGACACTCCCAGGAAGTGGACCACCTGA GGGAGGCTCTGCTTTCCTCAAGTTCCCAGCTCCAGGAGCTGGAGAAGGAGCTGGAGCAGCAGAAGGCTGA GCGGCGGCAGCTTCTGGAAGACTTGCAGGAGAAGCAGGATGAGATCGTGCATTTCCGAGAGGAGAGGCTG TCCCTCCAGGAAAACAACTCCAGGCTGCAGCACAAGCTGGCCCTCCTGCAACAACAGTGTGAGGAGAAAC AGCAGCTCTCCCTGTCCCTGCAGTCAGAGCTCCAGATCTACGAGTCCCTCTACGAAAATCCTAAGAAGGG CTTGAAAGCCTTCAGCCTAGATTCCTGTTACCAAGTCCCGGGTGAGTTGAGCTGCCTGGTGGCAGAGATT CGAGCTCTGAGAGTGCAGTTGGAGCAGAGCATTCAAGTGAACAACCGTCTGCGGCTGCAGCTGGAACAGC AGATGGATCACGGTGCTGGCAAAGCCAGTCTCAGTTCCTGCCCTGTTAACCAGAGCTTCTCAGCCAAGGC GGAGCTGGCAAACCAGCAGCCACCCTTCCAAGGTTCAGCTGCTTCCCCTCCAGTCCGGGACGTTGGCTTG AATTCTCCACCCGTGGTCCTCCCCAGCAATTCGTGCTCTGTTCCTGGCTCAGACTCTGCCATCATCAGTA GGACAAACAATGGTTCGGATGAGTCTGCAGCAACGAAGACCCCTCCCAAGATGGAGGTCGATGCTGCTGA TGGCCCATTTGCCAGTGGACACGGCAGACACGTCATCGGCCATGTGGATGACTACGACGCCCTACAGCAG CAGATTGGGGAAGGGAAGCTGCTGATCCAAAAGATACTGTCTCTCACGAGGCCAGCACGCAGCGTCCCTG CACTGGACGCGCAGGGCACAGAGGCACCAGGTACCAAAAGTGTCCATGAGCTTCGGAGCAGCGCCAGGGC TCTGAACCACAGCCTAGAAGAGTCAGCTTCCCTCCTCACCATGTTCTGGAGAGCAGCTTTGCCAAACTCT CATGGTTCTGTACTGGTAGGCGAAGAGGGAAACCTGATGGAGAAAGAACTCCTAGACCTGCGAGCCCAAG TGTCCCAACAGCAACAGCTCCTTCAGAGCACTGCTGTGCGTCTGAAGACGGCCAACCAGAGGAAGAAAAG CATGGAGCAGTTCATCGTGAGCCATCTGACCAGGACCCATGATGTCTTGAAGAAAGCACGGACTAATTTA GAGATGAAATCCTTCAGGGCCCTGATGTGCACTCCAGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214670 representing NM_022382 Red=Cloning site Green=Tags(s) MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEVSREDVYKRNIELKVEVESLKRELQDRKQHLH KTWADEEDLNSQNEAELRRQVEEPQQETEHVYELLDNNIQLLQEESRFAKDEATQMETLVEAEKGCNLEL SERWKDATKNREDAPGDQVKLDQYSAALAQRDRRIEELRQSLAAQEGLVEQLSREKQQLLHLLEEPGGME VQPMPKGLPTQQKPDLNETPTTQPSVSDSHLAELQDKIQQTEVTNKILQEKLNDMSCELRSAQESSQKQD TTIQSLKEMLKSRESETEELYQVIEGQNDTMAKLPEMLHQSQLGQLQSSEGIAPAQQQVALLDLQSALFC SQLEIQKLQRLLRQKERQLADGKRCMQFVEAAAQEREQQKEAAWKHNQELRKALQHLQGELHSKSQQLHV LEAEKYNEIRTQGQNIQHLSHSLSHKEQLIQELQELLQYRDTTDKTLDTNEVFLEKLRQRIQDRAVALER VIDEKFSALEEKDKELRQLRLAVRDRDHDLERLRCVLSANEATMQSMESLLRARGLEVEQLIATCQNLQW LKEELETKFGHWQKEQESIIQQLQTSLHDRNKEVEDLSATLLHKLGPGQSEVAEELCQRLQRKERVLQDL LSDRNKQAMEHEMEVQGLLQSMGTREQERQAVAEKMVQAFMERNSELQALRQYLGGKELMAASQAFISNQ PAGATSVGPHHGEQTDQGSTQMPSRDDSTSLTAREEASIPRSTLGDSDTVAGLEKELSNAKEELELMAKK ERESQIELSALQSMMAVQEEELQVQAADLESLTRNIQIKEDLIKDLQMQLVDPEDMPAMERLTQEVLLLR EKVASVEPQGQEGSENRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAQPETFERDRTLQVELE GAQVLRSRLEEVLGRSLERLSRLETLAAIGGATAGDETEDTSTQFTDSIEEEAAHNSHQQLIKVSLEKSL TTMETQNTCLQPPSPVGEDGNRHLQEEMLHLRAEIHQPLEEKRKAEAELKELKAQIEEAGFSSVSHIRNT MLSLCLENAELKEQMGEAMSDGWEVEEDKEKGEVMVETVVAKGGLSEDSLQAEFRKVQGRLKSAYNIINL LKEQLVLRSSEGNTKEMPEFLVRLAREVDRMNMGLPSSEKHQHQEQENMTARPGPRPQSLKLGTALSVDG YQLENKSQAQDSGHQPEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRAILSESL VKQDSKQIQVDLQDLGYETCGRSENEAEREETTSPECEEHGNLKPVVLVEGLCSEQGYLDPVLVSSPVKN PWRTSQEARGVQAQGTSDDSSLLRKDIRDLKAQLQNAYKVIQNLRSRVRSLSATSDYSSSLERPRKLRAV ATLEGASPHSVTDEDEGLLSDGTGAFYPPGLQAKKNLENLIQRVSQLEAQLPKTGLEGKLAEELKSASWP GKYDSLIQDQARKTVISASENTKREKDLFSSHPTFERYVKSFEDLLRNNDLTTYLGQSFREQLSSRRSVT DRLTSKFSTKDHKSEKEEAGLEPLALRLSRELQEKEKVIEVLQAKLDTRFSSPPSSHAASDSHRSASSTS FLSDDIEACSDMDVASEYTHYEEKKPSPSNSAASASQGLKGEPRSSSISLPTPQNPPKEASQAQPGFHFN SIPKPASLSQAPMHFTVPSFMPFGPSGPPLLGCCETPVVSLAEAQQELQMLQKQLGRSVSIAPPTSTSTL LSNHTEASSPRYSNPAQPHSPARGTIELGRILEPGYLGSGQWDMMRPQKGSISGELSSGSSMYQLNSKPT GADLLEEHLGEIRNLRQRLEESICVNDRLREQLQHRLSSTARENGSTSHFYSQGLESMPQLYNENRALRE ENQSLQTRLSHASRGHSQEVDHLREALLSSSSQLQELEKELEQQKAERRQLLEDLQEKQDEIVHFREERL SLQENNSRLQHKLALLQQQCEEKQQLSLSLQSELQIYESLYENPKKGLKAFSLDSCYQVPGELSCLVAEI RALRVQLEQSIQVNNRLRLQLEQQMDHGAGKASLSSCPVNQSFSAKAELANQQPPFQGSAASPPVRDVGL NSPPVVLPSNSCSVPGSDSAIISRTNNGSDESAATKTPPKMEVDAADGPFASGHGRHVIGHVDDYDALQQ QIGEGKLLIQKILSLTRPARSVPALDAQGTEAPGTKSVHELRSSARALNHSLEESASLLTMFWRAALPNS HGSVLVGEEGNLMEKELLDLRAQVSQQQQLLQSTAVRLKTANQRKKSMEQFIVSHLTRTHDVLKKARTNL EMKSFRALMCTPAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022382
ORF Size	6972 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022382.1, NP_071777.1
RefSeq Size	6975 bp
RefSeq ORF	6975 bp
Locus ID	64183
UniProt ID	Q9WUJ3
MW	262 kDa
Gene Summary	targets cyclic nucleotide phosphodiesterase PDE4D to the Golgi/centrosome; may play a role in cAMP-dependent intracellular signaling [RGD, Feb 2006]

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