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Uhrf1 (NM_001008882) Rat Tagged ORF Clone
CAT＃: RR214841
- TrueORF®
Uhrf1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like with PHD and ring finger domains 1 (Uhrf1), (10 ug)
"NM_001008882" in other vectors (3)
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CNY 10,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ac2-121; Np95 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214841 representing NM_001008882
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGGAATCTTTGGCGAGGGAGGATCCCTTCCTCCTCTCCCGGTATTGCTACGGGGTTTACAAG ACCTGGGCCTTCTTGGTAAACTAGATATAGCCCGCAGGGCCACACTGACTGGGATCTCCCTCTGTTTTTC AGTATTCCTTCAGCACATCGGCATCATGTGGATCCAGGTTCGAACTATGGATGGGAAGGAGACCCACACC GTGAACTCTCTATCCCGGTTGACCAAGGTGCAGGAGCTGAGGAAAAAGATTGAGGAGCTGTTTCACGTGG AACCCCAATTGCAGAGACTCTTTTACAGGGGCAAACAGATGGAGGATGGTCACACACTCTTCGATTATGA CGTGCGCCTCAATGACACAATCCAGCTGCTGGTGCGCCAGAGTCTGGCCCTACCTCTCAGTACGAAAGAA CGGGACTCGGAGCTCTCAGACTCTGACTCTGGCTATGGTGTGGGTCACAGTGAATCAGACAAGTCGTCCA CGCATGGTGAAGGGACAGCTGATGGGGATGACAAGACTGTGTGGGAGGACACTGACCTAGGACTGTACAA GGTTAATGAGTACGTGGACGTGCGTGACAATATCTTTGGTGCATGGTTTGAGGCCCAGGTGGTCCAGGTA CAGAAGAAAGCCCTATCTGAGGAAGAGCCCTGTAGCTCCAGTGCCATTATGGCCCCAGAGGATGACATCA TGTACCACATCAAGTATGATGACTATCCAGAGCATGGAGTGGACATTGTCAAAGCCAAGAATGTCCGTGC TCGCGCTCGCACTGTGATCCCCTGGGAGGACCTGGAGGTGGGTCAGGTGGTCATGGCCAACTACAACGTG GACTATCCCAGAAAACGTGGCTTCTGGTATGATGTTGAGATTTGTAGGAAGCGCCAAACCAGGACGGCAC GTGAACTGTATGGCAACGTCATGCTTTTGAATGATTCTCAGCTCAACAACTGTCGGATCATATTTGTGGA TGAAGTCTTGAAGATTGAGCTCCCTAATGAGAGGAGCCCCTTGATTGGTAGCCCCTCGAGACGGAAGAGC GGCCCATCCTGCCAGTACTGCAAGGATGACGAGAACAAACCGTGTCGCAAGTGTGCCTGCCATATTTGTG GTGGGCGTGAGGCTCCCGAGAAACAAGTGTTGTGTGATGAGTGTGACATGGCCTTCCACCTGTACTGCCT GCAGCCACCGCTCACCTGTGTCCCCCCTGAGCCAGAGTGGTACTGCCCCAGCTGCCGAACTGACTCCAGT GAGGTGGTACAAGCAGGGGAGAAACTGAAGAAAAGCAAGAAGAAGGCAAAGATGGCGTCAGCCACTTCCT CCTCCCGACGGGACTGGGGCAAGGGCATGGCATGTGTGGGCCGCACCACAGAGTGTACTATTGTACCGGC CAACCACTTCGGGCCCATCCCTGGTGTCCCCGTGGGCACCATGTGGCGCTTCAGAGTCCAGGTCAGTGAG TCCGGTGTGCATCGGCCACATGTGGCGGGTATTCATGGTCGGAGCAACGACGGTGCCTACTCACTGGTGC TGGCTGGTGGCTATGAGGATGATGTGGATAATGGCAATTTCTTCACCTACACGGGCAGTGGTGGCCGAGA CCTCTCTGGCAACAAGCGTACAGCGGGACAGTCCTCTGACCAGAAACTCACTAATAACAACAGGGCTCTG GCGCTCAATTGCCACTCCCCAATCAATGAGAAAGGTGCGGAGGCTGAAGACTGGCGCCAGGGTAAGCCGG TGCGTGTGGTCCGGAACATGAAGGGAGGCAAACATAGCAAGTACGCACCTGCAGAGGGGAACCGCTATGA TGGCATCTACAAGGTGGTCAAGTACTGGCCGGAGAAGGGGAAATCTGGCTTCATTGTGTGGCGCTATCTC CTTCGACGGGATGACACTGAACCTGAGCCGTGGACTCGGGAGGGCAAGGACCGCACTCGGCAGCTGGGCC TCACTATGCAGTATCCTGAAGGCTACTTGGAGGCCTTGGCTAACAAGGAGAAGAACAGGAAGCGCCCGGC CAAGGCCTTGGAGCAGGGACCATCATCTTCCAAGATAGGCAAAAGCAAGCGGAAGTCCACAGGCCCGGCC ACCACAAGCCCCCGTGTCTCCAAGAAGAGCAAGCTGGAGCCCTACACACTTCCATTGCAGCAGGCCAACC TCATCAAGGAGGACAAGGGCAACGCCAAGCTGTGGGATGACGTGCTGAGCTCCCTTCAGGATGGGCCGTA CCAGATCTTCCTGAGCAAGGTGAAGGAGGCTTTCCAGTGCATCTGTTGCCAGGAGTTGGTGTTCCGGCCA GTCACCACCGTGTGTCAGCACAACGTTTGTAAGGACTGCCTGGACAGGTCCTTCCGTGCCCAGGTGTTCA GCTGCCCAGCATGTCGCTATGACCTGGACCACAGCTCCCCAACCCGAGTGAATCAGCCCTTGCAGACCAT TCTCAACCAGCTCTTCCCTGGCTATGGCAGCGGCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214841 representing NM_001008882
Red=Cloning site Green=Tags(s) MESGIFGEGGSLPPLPVLLRGLQDLGLLGKLDIARRATLTGISLCFSVFLQHIGIMWIQVRTMDGKETHT VNSLSRLTKVQELRKKIEELFHVEPQLQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVRQSLALPLSTKE RDSELSDSDSGYGVGHSESDKSSTHGEGTADGDDKTVWEDTDLGLYKVNEYVDVRDNIFGAWFEAQVVQV QKKALSEEEPCSSSAIMAPEDDIMYHIKYDDYPEHGVDIVKAKNVRARARTVIPWEDLEVGQVVMANYNV DYPRKRGFWYDVEICRKRQTRTARELYGNVMLLNDSQLNNCRIIFVDEVLKIELPNERSPLIGSPSRRKS GPSCQYCKDDENKPCRKCACHICGGREAPEKQVLCDECDMAFHLYCLQPPLTCVPPEPEWYCPSCRTDSS EVVQAGEKLKKSKKKAKMASATSSSRRDWGKGMACVGRTTECTIVPANHFGPIPGVPVGTMWRFRVQVSE SGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDNGNFFTYTGSGGRDLSGNKRTAGQSSDQKLTNNNRAL ALNCHSPINEKGAEAEDWRQGKPVRVVRNMKGGKHSKYAPAEGNRYDGIYKVVKYWPEKGKSGFIVWRYL LRRDDTEPEPWTREGKDRTRQLGLTMQYPEGYLEALANKEKNRKRPAKALEQGPSSSKIGKSKRKSTGPA TTSPRVSKKSKLEPYTLPLQQANLIKEDKGNAKLWDDVLSSLQDGPYQIFLSKVKEAFQCICCQELVFRP VTTVCQHNVCKDCLDRSFRAQVFSCPACRYDLDHSSPTRVNQPLQTILNQLFPGYGSGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008882 |
| ORF Size | 2487 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008882.1, NP_001008882.1 |
| RefSeq Size | 2536 bp |
| RefSeq ORF | 2490 bp |
| Locus ID | 316129 |
| UniProt ID | Q7TPK1 |
| MW | 93.2 kDa |
| Gene Summary | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214841 | Uhrf1 (untagged ORF) - Rat ubiquitin-like with PHD and ring finger domains 1 (Uhrf1), (10 ug) |
CNY 10,640.00 |
|
| RR214841L3 | Lenti ORF clone of Uhrf1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like with PHD and ring finger domains 1 (Uhrf1), (10 ug) |
CNY 12,450.00 |
|
| RR214841L4 | Lenti ORF clone of Uhrf1 (mGFP-tagged ORF) - Rat ubiquitin-like with PHD and ring finger domains 1 (Uhrf1), (10 ug) |
CNY 13,590.00 |
