Col5a2 (NM_053488) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217645 representing NM_053488 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCAAACTGGGTGGTAGCAAGACCTCTCCTCATTCTTTTTGTTTTATTAGGGTACTGTGTCTCAA TAAAAGCCCAGGAACAAGAGAATGATGACTATGATGAAGAAATAGCCTGTACACAACATGGCCAGATGTA TTTAAATAGAGACATTTGGAAACCTTCTCCATGCCAGATCTGTGTCTGTGACAATGGTGCTATCCTCTGT GACAAGATAGAGTGCCAAGAAGTGCTCAACTGTGCCAATCCTGTAACACCTCCTGGGGAGTGCTGCCCAG CGTGTCCACAGACATCTGGAGGTGGAGATACAAGTTTTGGTAGAGGAAGAAAGGTGAAAGGCTTACCAGG GTATTATGAAATTGTAACGGGCATACGTGGTCGTCCAGGACCTGCAGGCCCTCCAGGATCACAAGGACCA AGAGGGGACCGAGGCCCCAAAGGAAGACCTGGTCCTCGTGGTCCTCAGGGAATTGATGGAGAGCCAGGTG TTCCTGGTCAGCCAGGTGCTCCAGGGCCTCCTGGGCACCCCTCTCACCCAGGACCTGATGGCATGAGCAG GCCCTTTTCAGCTCAAATGGCTGGGTTGGATGAAAAATCTGGACTTGGAAGTCAAGTGGGACTCATGCCT GGCTCTGTGGGTCCTATTGGCCCAAGGGGACCTCAGGGTTTACAAGGACAGCAAGGTGGTGTGGGACCTG CAGGACCTCCTGGTGAGCCTGGCGAGCCTGGACCAATGGGTCCAATTGGTGCCCGTGGACCAGAAGGCCC ACCTGGGAAGCCTGGAGAAGATGGTGAACCTGGAAGAAATGGGAATACAGGTGAAGTGGGGTTCTCAGGA TCACCAGGTGCTCGAGGCTTTCCTGGTGCTCCTGGTCTCCCTGGCTTGAAAGGTCACAGAGGAAACAAAG GTCTTGAAGGCCCCAAAGGTGAAGTTGGAGCACCTGGTGCTAAGGGTGAAGCTGGCCCCACCGGTCCAAT GGGTGCTATGGGCCCTCTGGGACCAAGAGGAATGCCAGGAGAGAGGGGAAGACTTGGGCCACAGGGTGCT CCTGGACAACGAGGTGCTCATGGCATGCCTGGAAAACCTGGACCAATGGGTCCCCTTGGGATACCTGGCT CTTCTGGCTTTCCAGGAAATCCTGGAGTGAAGGGAGAAGCAGGTCCTACTGGGGCCCGAGGCCCTGAAGG ACCTCAGGGACAGAGAGGTGAAACTGGGCCACCTGGTCCTGCTGGCTCTCATGGCCTTCCTGGTGCGGTG GGGACTGATGGTACACCTGGCGCTAAAGGACCCACAGGCTCTCCTGGAACCTCTGGCCCTCCTGGCTTAG CAGGGCCCCCTGGGTCTCCTGGGCCTCAGGGCAGCACTGGACCTCAGGGAATTCGAGGACAACTGGGTGA TCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGCCCAAAAGGAGAACCAGGGCCACATGGTATTCAGGGT CCTATTGGTCCACCTGGAGAAGAAGGCAAAAGAGGTCCTCGAGGGGACCCAGGCACAGTTGGTCCTCCAG GACCAATGGGAGAAAGGGGTGCTCCTGGCAATCGTGGTTTTCCAGGTTCTGATGGTTTACCTGGACCAAA GGGTGCTCAGGGAGAGCGTGGCCCTATAGGTTCTTCAGGGCCTAAAGGAGGCCAGGGAGATCCAGGACGT CCAGGCGAACCTGGACTTCCAGGTGCTCGGGGCCTGACAGGAAATCCTGGTGTTCAAGGTCCTGAAGGAA AACTTGGGCCTTTGGGTGCTCCTGGCGAAGATGGCCGACCAGGTCCTCCAGGCTCCATTGGTATTAGAGG GCAGCCTGGAAGCATGGGCCTTCCAGGCCCCAAAGGTAGCAGTGGTGACCTTGGGAAACCAGGAGAAGCT GGGAATGCTGGCGTGCCTGGGCAAAGGGGGGCTCCTGGCAAAGACGGAGAAGTTGGTCCTTCAGGCCCCG TGGGACCTCCGGGCCTAGCTGGGGAAAGAGGAGAACAGGGACCTCCAGGTCCTACTGGCTTTCAGGGACT TCCTGGGCCTCCAGGGCCACCTGGAGAAGGTGGAAAGCCTGGTGATCAAGGTGTTCCTGGAGAGCCTGGA GCTGTTGGGCCATTAGGACCTCGGGGAGAAAGAGGGAATCCTGGAGAAAGAGGAGAGCCAGGGATAACTG GATTACCTGGGGAGAAAGGCATGGCTGGAGGACATGGCCCTGATGGTCCAAAGGGCAACCCAGGTCCAAC TGGGACCATTGGAGACACAGGGCCACCAGGTCTTCAAGGCATGCCAGGAGAAAGAGGAATTGCAGGGACA CCTGGTCCTAAGGGTGACAGAGGTGGCGTAGGAGAGAAAGGTGCTGAAGGAACAGCTGGAAATGATGGGG CAAGGGGTCTTCCTGGTTCCTTGGGACCTCCAGGTCCTGCAGGCCCTACTGGAGAAAAGGGTGAACCTGG TCCTAGAGGATTAGTTGGCCCTCCTGGCTCCCGTGGCAATCCTGGTTCTCGGGGGGAAAATGGTCCAACT GGAGCTGTTGGTTTTGCTGGACCCCAGGGTCCCGATGGACAGCCTGGTGTCAAGGGTGAGCCTGGAGAGC CAGGGCAGAAGGGAGATGCTGGGTCTCCTGGACCCCAAGGACTTGCAGGATCACCTGGCCCTCATGGTCC TCATGGTGTTCCTGGACTGAAAGGTGGTCGAGGAACCCAGGGTCCACCTGGTGCTACAGGATTTCCTGGT TCTGCTGGAAGAGTTGGGCCTCCAGGCCCCGCTGGAGCTCCAGGACCTGCAGGGCCCATAGGAGAACCTG GGAAGGAAGGACCACCCGGTCTTCGTGGGGACCCTGGCTCTCATGGGAGAGTGGGTGATAGAGGACCAGC TGGCCCACCTGGTAGCCCAGGAGACAAAGGGGACCCAGGGGAAGATGGACAACCTGGTCCAGATGGCCCC CCTGGTCCTGCTGGTACCACTGGGCAAAGAGGAATTGTTGGAATGCCTGGACAACGTGGGGAGCGAGGAA TGCCTGGCCTTCCTGGCCCAGCGGGTACACCAGGAAAAGTAGGACCGACTGGTGCAACAGGAGATAAAGG TCCCCCTGGACCCGTAGGACCTCCAGGTTCCAATGGTCCTGTGGGGGAACCTGGACCAGAAGGTCCGGCT GGTAATGATGGTACCCCAGGACGGGATGGTGCTGTTGGAGAACGGGGTGACCGTGGAGACCCCGGGCCTG CTGGTCTACCAGGTTCTCAGGGTGCCCCTGGGACCCCTGGCCCTGTCGGTGCACCAGGAGATGCAGGACA AAGAGGAGAGCCAGGTTCCCGAGGCCCTGTAGGACCACCTGGTCGAGCTGGAAAGCGTGGTTTACCTGGA CCACAAGGACCTCGAGGTGATAAGGGTGACAATGGAGACAGAGGTGACAGAGGTCAGAAAGGCCACAGAG GCTTCACTGGCTTGCAGGGTCTTCCTGGCCCTCCTGGTCCAAATGGTGAACAAGGAAGTGCTGGCATTCC TGGTCCTTTTGGCCCAAGAGGTCCTCCAGGACCAGTAGGTCCTTCAGGAAAAGAAGGAAACCCTGGGCTA CTTGGACCAATTGGACCTCCTGGTGTGCGGGGAAGTGTAGGAGAAGCAGGACCAGAGGGCCCTCCTGGTG AACCTGGCCCTCCTGGCCCTCCAGGTCCCCCTGGTCATCTTACTGCTGCTCTTGGTGATATCATGGGACA CTATGATGAGAACATGCCAGATCCACTTCCAGAATTTACAGAAGACCAGGCAGCTCCAGATGACACAAAC AAAACTGACCCTGGAGTCCATGCTACTCTGAAGTCTCTCAGTAGTCAAATTGAAACCATGCGGAGTCCTG ATGGTTCCAAGAAGCACCCAGCCCGCACTTGTGATGATTTAAAGCTTTGCCATCCTACAAAGCAGAGTGG TGAATATTGGATTGATCCTAACCAGGGATCAGCTGAAGACGCAATCAAAGTTTACTGCAATCTGGAGACA GGAGAGACATGCATTTCCGCAAACCCAGCCAGTGTTCCACGGAAAACGTGGTGGGCCAGCAAATCCCCTG ACAATAAGCCCGTGTGGTATGGTCTCGACATGAATAGAGGATCTCAGTTCACATATGGAGATTACAAGTC CCCTAACACAGCTATTACTCAGATGACCTTTCTGCGCCTTTTATCAAAAGAAGCCTCCCAGAACCTTACT TATATATGTAAGAACACTGTTGGTTATATGGATGATCAAACTAAAAACCTCAAGAAAGCTGTGGTTCTTA AAGGGTCAAATGACTTAGAAATAAAAGGAGAAGGAAACATCAGATTCAGATACACCGTTCTTCAAGATAC CTGTTCAAAGCGAAATGGAAATGTAGGCAAGACTGTCTTTGAATACAGAACACAGAATGTGGCCCGCTTG CCCATCATAGATATCGGTCCTGTGGATATTGGCAATGCAGACCAGGAATTTGGCTTTGAAATTGGGCCAG TTTGTTTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217645 representing NM_053488 Red=Cloning site Green=Tags(s) MMANWVVARPLLILFVLLGYCVSIKAQEQENDDYDEEIACTQHGQMYLNRDIWKPSPCQICVCDNGAILC DKIECQEVLNCANPVTPPGECCPACPQTSGGGDTSFGRGRKVKGLPGYYEIVTGIRGRPGPAGPPGSQGP RGDRGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMP GSVGPIGPRGPQGLQGQQGGVGPAGPPGEPGEPGPMGPIGARGPEGPPGKPGEDGEPGRNGNTGEVGFSG SPGARGFPGAPGLPGLKGHRGNKGLEGPKGEVGAPGAKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGA PGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGVKGEAGPTGARGPEGPQGQRGETGPPGPAGSHGLPGAV GTDGTPGAKGPTGSPGTSGPPGLAGPPGSPGPQGSTGPQGIRGQLGDPGVPGFKGEAGPKGEPGPHGIQG PIGPPGEEGKRGPRGDPGTVGPPGPMGERGAPGNRGFPGSDGLPGPKGAQGERGPIGSSGPKGGQGDPGR PGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDLGKPGEA GNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGEPG AVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGNPGPTGTIGDTGPPGLQGMPGERGIAGT PGPKGDRGGVGEKGAEGTAGNDGARGLPGSLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPT GAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPHGVPGLKGGRGTQGPPGATGFPG SAGRVGPPGPAGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGSPGDKGDPGEDGQPGPDGP PGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPA GNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGEPGSRGPVGPPGRAGKRGLPG PQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGL LGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDENMPDPLPEFTEDQAAPDDTN KTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPTKQSGEYWIDPNQGSAEDAIKVYCNLET GETCISANPASVPRKTWWASKSPDNKPVWYGLDMNRGSQFTYGDYKSPNTAITQMTFLRLLSKEASQNLT YICKNTVGYMDDQTKNLKKAVVLKGSNDLEIKGEGNIRFRYTVLQDTCSKRNGNVGKTVFEYRTQNVARL PIIDIGPVDIGNADQEFGFEIGPVCFM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053488
ORF Size	4491 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053488.1, NP_445940.1
RefSeq Size	6085 bp
RefSeq ORF	4494 bp
Locus ID	85250
MW	145.3 kDa
Gene Summary	alpha chain of type V collagen; has a role in bone growth [RGD, Feb 2006]

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