Lamc1 (NM_053966) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B2e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217665 representing NM_053966 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGCGGCAGGCGGGCCGCTCTGGCCCTGCAGCTCCAGCGGCGGCTGTGGCTGCTGCTGGCTGTGC TGGCGGCCGTGGCGTGCTGGGTCCGGGCGGCCATGGACGAGTGCGCGGATGAGGGCGGCCGGCCGCAGCG CTGCATGCCGGAGTTTGTTAATGCCGCCTTCAATGTGACCGTGGTGGCTACCAACACGTGTGGGACTCCA CCCGAGGAGTACTGCGTGCAGACTGGAGTGACCGGAGTCACCAAGTCCTGTCACCTGTGCGACGCCGGCC AGCTCCACCTGCAACACGGGGCAGCCTTCTTGACCGACTACAACAACCAGGCCGACACCACCTGGTGGCA AAGCCAGACTATGCTGGCCGGGGTGCAGTACCCCAACTCCATCAACCTCACGCTGCACCTGGGAAAGGCT TTTGACATCACTTACGTGCGTCTCAAGTTCCACACCAGCCGCCCAGAGAGTTTCGCCATTTATAAGCGCA CTCGGGAAGACGGGCCCTGGATTCCTTACCAGTACTACAGTGGCTCCTGTGAGAACACGTACTCAAAGGC TAACCGTGGCTTCATCAGGACGGGAGGAGATGAGCAGCAGGCCTTGTGTACCGATGAGTTCAGTGACATT TCCCCCCTCACCGGCGGCAACGTGGCCTTTTCAACTCTGGAAGGAAGGCCGAGTGCCTACAACTTTGACA ACAGCCCCGTGCTACAGGAATGGGTGACAGCCACTGACATCAGAGTCACACTCAATCGCCTGAACACCTT TGGAGATGAAGTATTCAATGACCCCAAAGTCCTCAAGTCTTACTATTATGCAATCTCGGACTTTGCTGTG GGTGGCAGGTGTAAATGTAATGGACATGCCAGTGAGTGTGTAAAGAACGAGTTTGGGAAGCTCGTGTGCA ACTGCAAGCATAACACATACGGAGTTGACTGTGAAAAGTGCCTGCCTTTCTTCAACGATCGGCCGTGGAG GAGAGCAACTGCCGAGAGTGCCAACCAGTGCCTGCCTTGCGACTGCAATGGCCGATCCCAAGAATGCTAC TTTGACCCTGAGCTATACCGTTCCACTGGGCACGGTGGCCACTGTACCAACTGCCGGGATAACACAGACG GTGCCAAGTGCGAGAGGTGCCGGGAGAACTTCTTCCGCCTGGGGAACACTGAAGCCTGCTCTCCGTGCCA CTGCAGCCCCGTTGGTTCTCTCAGCACGCAGTGTGACAGTTACGGCAGGTGCAGCTGTAAGCCCGGAGTG ATGGGTGACAAGTGTGACCGTTGCCAGCCTGGGTTCCATTCCCTCACCGAGGCAGGATGCAGGCCATGCT CCTGCGACCCTTCTGGCAGCACCGACGAATGTAATGTTGAAACAGGAAGATGCGTTTGCAAAGACAACGT CGAAGGCTTCAGCTGTGAGAGATGCAAACCTGGATTTTTTAATCTGGAATCCTCCAATCCTAAGGGTTGC ACACCCTGCTTCTGCTTTGGGCATTCTTCTGTGTGCACCAATGCTGTTGGCTACAGTGTTTATGACATCT CCTCGACCTTTCAGATTGATGAGGACGGGTGGCGTGTGGAGCAGAGAGATGGCTCGGAGGCGTCTCTTGA GTGGTCCTCAGACCGGCAGGATATTGCTGTGATCTCAGACACTTACTTCCCCAGATACTTTGTCGCCCCT GCCAAGTTCCTGGGCAACCAGGTCCTGAGTTACGGGCAGAACCTCTCATTCTCCTTCCGAGTGGACAGAC GGGACACTCGCCTCTCCGCAGAGGATCTCGTGCTGGAAGGAGCTGGCTTGAGAGTATCCGTGCCCTTGAT CGCTCAGGGCAACTCGTACCCTAGCGAGACCACTGTGAAGTACATCTTCAGACTCCATGAAGCAACAGAT TACCCTTGGAGGCCTGCTCTCTCCCCATTTGAATTTCAGAAGCTCCTAAACAACTTGACCTCTATCAAGA TCCGTGGTACATACAGTGAGAGGAGCGCTGGATATTTGGATGATGTCACCTTGCAAAGTGCTCGCCCTGG GCCCGGAGTCCCCGCAACGTGGGTGGAGTCCTGCACCTGTCCAGTGGGGTATGGAGGGCAGTTCTGTGAG ACATGCCTCCCAGGCTACAGAAGAGAAACCCCAAGTCTTGGACCTTACAGCCCGTGTGTGCTCTGTACCT GTAATGGGCACAGTGAGACCTGTGACCCCGAGACAGGTGTCTGTGACTGCAGAGACAATACTGCCGGCCC GCACTGTGAGAAATGTAGCGATGGGTACTATGGAGACTCAACCCTGGGCACCTCCTCTGACTGCCAGCCT TGTCCCTGCCCTGGCGGCTCAAGTTGTGCCATTGTCCCAAAGACAAAGGAAGTGGTGTGCACACACTGTC CAACTGGCACTGCCGGCAAGAGATGTGAGCTCTGTGATGACGGCTACTTTGGAGACCCTCTGGGCAGCAA TGGACCCGTGAGACTCTGCCGCCCATGTCAGTGCAACGACAACATAGACCCCAACGCAGTTGGCAACTGC AACCGTCTGACTGGAGAATGCCTGAAGTGCATCTATAACACGGCTGGCTTCTACTGCGACCGGTGCAAAG AAGGGTTTTTCGGAAATCCCCTGGCTCCCAATCCAGCAGACAAGTGCAAAGCCTGCGCCTGCAATCCCTA TGGTACAGTACAGCAGCAGAGCAGCTGTAACCCAGTGACCGGGCAGTGCCAGTGTCTGCCTCATGTGTCA GGCCGGGACTGTGGCGCTTGTGACCCTGGCTTCTACAACCTGCAGAGCGGGCAAGGCTGCGAGAGTTGTG ACTGCCATGCTTTGGGCTCCACCAACGGGCAATGTGACATCCGCACCGGGCAGTGTGAGTGTCAGCCCGG CATCACCGGTCAGCACTGTGAGCGCTGTGAGACCAACCACTTTGGGTTTGGACCTGAAGGCTGCAAACCT TGCGACTGTCACCACGAAGGATCCCTTTCGCTCCAATGTAAGGATGACGGCCGTTGTGAATGCAGGGAAG GCTTCGTGGGCAATCGCTGTGACCAGTGTGAAGAGAACTATTTCTACAATCGGTCTTGGCCTGGCTGCCA AGAGTGTCCGGCTTGTTACCGGCTGGTGAAGGATAAGGTTGCTGAGCATCGAGTGAAACTCCAGGAGTTG GAGAGCCTCATAGCAAACCTCGGGACTGGGGATGAGACGGTGACAGATCAAGCCTTTGAGGACAGACTGA AGGAAGCAGAGAGGGAGGTTACAGACCTCCTCCGTGAGGCTCAGGAAGTCAAAGATGTAGACCAAAATCT GATGGATCGCCTTCAGAGAGTAAATAACAGTCTGCACAGCCAAATCAGCCGGCTGCACAATATCCGGAAC ACTATAGAAGAGACGGGGATCTTGGCTGAGCGAGCGCGGTCCCGAGTGGAGAGCACAGAGCAGCTGATTG AGATCGCCTCCAGGGAGCTCGAGAAAGCAAAAATGGCTGCCGCCAATGTGTCAATCACTCAGCCAGAGTC TACAGGGGAACCAAACAACATGACCCTCTTGGCAGAAGAAGCCCGAAGGCTTGCAGAGCGTCATAAACAG GAAGCTGATGACATTGTACGAGTGGCAAAGACAGCCAACGAGACCTCAGCTGAGGCATATAATCTGCTTT TGAGGACCCTGGCAGGAGAAAATCAAACTGCATTGGAGATTGAAGAGCTTAACAGGAAGTATGAACAAGC AAAGAACATCTCTCAGGACCTGGAGAAGCAGGCTGCCAGAGTCCATGAGGAAGCCAAGCGAGCAGGTGAC AAAGCCGTGGAGATCTATGCCAGTGTGGCCCAGCTGACCCCTGTGGACTCGGAGGCTCTAGAGAGTGAAG CAAATAAAATCAAGAAAGAAGCTGCAGATCTGGACCGTCTGATTGACCAGAAGCTAAAAGATTACGAGGA CCTTAGGGAAGACATGCGAGGAAAGGAACATGAAGTAAAGAACCTTCTAGAGAAGGGGAAAGCCGAACAG CAGACCGCTGACCAACTCCTAGCTCGAGCCGACGCTGCCAAGGCTCTTGCTGAAGAAGCTGCTAAAAAGG GACGCAATACCTTACAAGAAGCTAACGACATTCTCAACAACCTGAAAGATTTTGATAGACGAGTGAACGA TAACAAGACAGCTGCTGAAGAAGCTCTAAGGAGAATTCCCGCCATCAACCGGACCATAGCTGAAGCCAAT GAGAAGACAAGGGAGGCACAGCTGGCGCTGGGCAATGCAGCAGCTGACGCCACGGAGGCCAAGAACAAAG CCCACGAGGCGGAGAGGATTGCCAGTGGCGTGCAGAAGAATGCCACCAGTACCAAGGCGGATGCGGAGAG AACCTTCTCGGAAGTGACAGACCTGGATAACGAGGTGAATGGCATGTTGAGGCAGCTAGAGGAAGCGGAG AATGAGCTGAAGAGGAAGCAAGATGATGCCGACCAGGACATGATGATGGCGGGAATGGCTTCACAGGCTG CTCAGGAAGCTGAACTTAATGCCAGAAAAGCCAAAAACTCTGTCAGCAGCCTCCTCAGCCAACTGACCGA CCTCTTGGAGCAGCTGGGACAGCTGGACACCGTGGACCTGAACAAGCTCAACGAGATCGAAGGCTCTCTG AACAAAGCCAAAGACGAAATGAAGGTGAGCGACCTGGACAGGAAGGTGTCCAACCTGGAGAACGAGGCCC GGAAGCAGGAGGCAGCCATCTTGGACTATGACCGAGACATAGCAGAGATCATAAAGGACATTCACAACCT GGAGGACATCAAGAAGACACTACCAACAGGCTGCTTTAACACCCCGTCCATCGAGAAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217665 representing NM_053966 Red=Cloning site Green=Tags(s) MTGGRRAALALQLQRRLWLLLAVLAAVACWVRAAMDECADEGGRPQRCMPEFVNAAFNVTVVATNTCGTP PEEYCVQTGVTGVTKSCHLCDAGQLHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPNSINLTLHLGKA FDITYVRLKFHTSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGFIRTGGDEQQALCTDEFSDI SPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYAISDFAV GGRCKCNGHASECVKNEFGKLVCNCKHNTYGVDCEKCLPFFNDRPWRRATAESANQCLPCDCNGRSQECY FDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEACSPCHCSPVGSLSTQCDSYGRCSCKPGV MGDKCDRCQPGFHSLTEAGCRPCSCDPSGSTDECNVETGRCVCKDNVEGFSCERCKPGFFNLESSNPKGC TPCFCFGHSSVCTNAVGYSVYDISSTFQIDEDGWRVEQRDGSEASLEWSSDRQDIAVISDTYFPRYFVAP AKFLGNQVLSYGQNLSFSFRVDRRDTRLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYIFRLHEATD YPWRPALSPFEFQKLLNNLTSIKIRGTYSERSAGYLDDVTLQSARPGPGVPATWVESCTCPVGYGGQFCE TCLPGYRRETPSLGPYSPCVLCTCNGHSETCDPETGVCDCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQP CPCPGGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNC NRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVS GRDCGACDPGFYNLQSGQGCESCDCHALGSTNGQCDIRTGQCECQPGITGQHCERCETNHFGFGPEGCKP CDCHHEGSLSLQCKDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKVAEHRVKLQEL ESLIANLGTGDETVTDQAFEDRLKEAEREVTDLLREAQEVKDVDQNLMDRLQRVNNSLHSQISRLHNIRN TIEETGILAERARSRVESTEQLIEIASRELEKAKMAAANVSITQPESTGEPNNMTLLAEEARRLAERHKQ EADDIVRVAKTANETSAEAYNLLLRTLAGENQTALEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGD KAVEIYASVAQLTPVDSEALESEANKIKKEAADLDRLIDQKLKDYEDLREDMRGKEHEVKNLLEKGKAEQ QTADQLLARADAAKALAEEAAKKGRNTLQEANDILNNLKDFDRRVNDNKTAAEEALRRIPAINRTIAEAN EKTREAQLALGNAAADATEAKNKAHEAERIASGVQKNATSTKADAERTFSEVTDLDNEVNGMLRQLEEAE NELKRKQDDADQDMMMAGMASQAAQEAELNARKAKNSVSSLLSQLTDLLEQLGQLDTVDLNKLNEIEGSL NKAKDEMKVSDLDRKVSNLENEARKQEAAILDYDRDIAEIIKDIHNLEDIKKTLPTGCFNTPSIEKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053966
ORF Size	4821 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053966.2, NP_446418.1
RefSeq Size	7636 bp
RefSeq ORF	4824 bp
Locus ID	117036
MW	177.4 kDa
Gene Summary	an extracellular matrix protein [RGD, Feb 2006]

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