RPL35A (AK055653) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DBA5; L35A
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK055653, the custom clone sequence may differ by one or more nucleotides TACAAAACTCAAATGTTAAATTTGTCTGAAAAATATATTTCTACATGATCTGATAAAGTTCAGATAAGGG GCATTCTGTTCCTAAGAATGTCCTAAAAATGGAAAGCTGATAAAAGATTACGCTTAGAATTATACAGGTA AAAGACCTACAACAGTTGTTCTCTTGTTTTGATCCCATAGGTTATTTGTTATTACTCTTCTTTAACAGAG TGATTTTCTATTCTTAACCTTCTTAAGAGCCAATACAGAGTAGAAAGAAATATTTTCAGAAAGCCTTTTT AATAGTAACTTAATGTAAAAATTGTTTATCCTTTGGTTGAGGTTTCCCATTTCCATTTTTAAGCTTATCT GAACTGTGAACATGAAAAACGTAATGATTCATTGGTCAAGAAGCTACCATTTGAGGGCTCTAAAGTTTCT TTTAGATTTCCTTTTTTATATCAGTACAATTAAAATTTGATTTATCAAGTAGCAGCAGCTTCTTGATATT GTTACATACATTAGCTTGTTCATAAAATGTTAGAGTTAAAACTCATTAAAAATGCTATCGTTAAACATAA ACTAATTAAAATTCAGTATACGAGATTCTCCCTTCAAAAACATCTTATTTTTATTTTATTTAGTACTTGC TTCAGTGTTTTCTAGGAATATCTAGTAAAGGCCTTTACTGTTTTCCACATGGCCTAATTTTATATTGGAC TACAATTTTTGAGAAGGAATGCCAAATACAAAATTTTGAATCAATAAATTACAATTATAATTTTTATACT TATTTTCAAAGTTAATTTACTTTAGATGGCAGTAAAATCACTGAACTTCTGATTGAAAGAAAATTTTTTA GTTTGAAATTTCCTTCTGGAAAGGGGTTTGCAGTCCCTTTGGAAAGGAGTAAGCAATCCTGGAAGTAATT GAGTATTTAATCCTCCTATATTTTTTCTGAAGATTTTTGTGTCAGTATACTTTCTTTATGCTGGCTAGAA AGATCAGTTCTCAAGAGTTGCAGGATAAAATCAAATTAACTTTCATGTTTCAGAACTAAAACATATCCTT GCATTTATGGGAAGGATAGGTAAAAATGGAGATGGTGGCTGATAAAATAAAAATCTTTGAAACTATGTTT TTACAAGCCCAAAGCCTTTTGGAGTTGAAAGTATTAAAAAAAAGTATGATATGCTATCTTCAGGTTATGT ATATGATCACAGATAATAGCTCTGATGGTCACAGAGCTTGCTGATGGCTTCCAAAGGATGGCTCCTGCCT TTTACCTTGTTACCACAGCTCCTCATAGGGCACTTTGAGTGGCAGTTCTGGAATCTGAAAAAGAAAGGAT AGTGTAAGAATGGTGGTAAAATAAAAAGAACTGTCTTTATAGTTCCTGCTTTATAGGCTCAATAAGGATT TTTCTCTTACCGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTTCTAAAAGGAACTATGTCT GGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGCGGGGTCTCCGGAACCAAAGGGAGCACACAG CTCTTCTTAAAATTGAAGGTGTTTACGCCCGAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTA TGTATATAAAGCAAAGAACAACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGA AAAGTAACTCGGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAGTCAATAAATAA ATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACCTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK055653 unedited CGGCCGCGAATTCGGCACCAGGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGAC TTCTAAAAGGAACTATGTCTGGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGC GGGGTCTCCGGAACCAAAGGGAGCACACAGCTCTTCTTAAAATTGAAGGTGTTTACGCCC GAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTATGTATATAAAGCAAAGAACA ACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGAAAAGTAACTC GGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAAT CAATAAATAAATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACC TTAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCT GAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAG TTGCCACTCCAGTGCCCACCAGCCTTGTCCTTAATAAATTAAGTTGCATCATTTTGTCTG ACTAGGTGTCCTCTTAATATAATGGGTGAGGGGGGGGGTTTTTTTAAACCAGGGCCA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK055653 unedited NTCCGGTTCATCTATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTT TTAAGGTAGTAAGTTTTTTAATCCACTTAAAATACAAGAGCACAAATCCACATTTATTTA TTGATTTTTCGTTAGTTTAAATCCTTGAGGGGTACAGCATCACTCGGATTCTGTGTCCAA TGGCCTTAGCAGGAAGATTGCTTCGGAATTTGGCACGAACCATGCCACTGTTTCCATGGG CCCGAGTTACTTTTCCCCAGATGACTCTGGTTTTGTTTGGTTTGCCGCCAGGAGTGACTG TGTTGTTCTTTGCTTTATATACATAAGCGCATCTCTTGCCCAAATAGAATTCTGTTTCAT CTCGGGCGTAAACACCTTCAATTTTAAGAAGAGCTGTGTGCTCCCTTTGGTTCCGGAGAC CCCGCTTATAGCCAGCAAAAATGGCCTTGGACCACAGCCTTCCAGACATAGTTCCTTTTA GAAGTCCCGTTCCCAGCAGGCCTCCACAGGAGCCAAGATGGCCTGGTGCCGAATTCGCGG CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG GTGGAGACTTGGAAATCCCCGTGAGTCANACCGCTATCCACGCCCATTGGTGTACTGCCA AAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTANGAAA GTCCCGNTAGGTCATGTACTGGGCATAAATGCCAGCGGGCCATTTACCGTCATTGACA
Restriction Sites	NotI-NotI
ACCN	AK055653
Insert Size	500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK055653.1
RefSeq Size	1872 bp
RefSeq ORF	1872 bp
Locus ID	6165
Domains	Ribosomal_L35Ae
Protein Pathways	Ribosome
Gene Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]

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