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PYGL (NM_002863) Human Untagged Clone
CAT＃: SC118381
PYGL (untagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1
CNY 6,208.00
CNY 7,220.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GSD6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118381 sequence for NM_002863 edited (data generated by NextGen Sequencing)
ATGGCGAAGCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTG GGCGTGGAGAACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTG GTCAAGGACCGCAACGTGGCCACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTG CGCGACCACCTGGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTACGACAAGTGCCCC AAGAGGGTATATTACCTCTCTCTGGAATTTTACATGGGCCGAACATTACAGAACACCATG ATCAACCTCGGTCTGCAAAATGCCTGTGATGAGGCCATTTACCAGCTTGGATTGGATATA GAAGAGTTAGAAGAAATTGAAGAAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTT GCTGCCTGCTTCTTGGATTCCATGGCAACCCTGGGACTTGCAGCCTATGGATACGGCATT CGGTATGAATATGGGATTTTCAATCAGAAGATCCGAGATGGATGGCAGGTAGAAGAAGCA GATGATTGGCTCAGATATGGAAACCCTTGGGAGAAGTCCCGCCCAGAATTCATGCTGCCT GTGCACTTCTATGGAAAAGTAGAACACACCAACACCGGGACCAAGTGGATTGACACTCAA GTGGTCCTGGCTCTGCCATATGACACCCCCGTGCCCGGCTACATGAATAACACTGTCAAC ACCATGCGCCTCTGGTCTGCTCGGGCACCAAATGACTTTAACCTCAGAGACTTTAATGTT GGAGACTACATTCAGGCTGTGCTGGACCGAAACCTGGCCGAGAACATCTCCCGGGTCCTC TATCCCAATGACAATTTTTTTGAAGGGAAGGAGCTAAGATTGAAGCAGGAATACTTTGTG GTGGCTGCAACCTTGCAAGATATCATCCGCCGTTTCAAAGCCTCCAAGTTTGGCTCCACC CGTGGTGCAGGAACTGTGTTTGATGCCTTCCCGGATCAGGTGGCCATCCAGCTGAATGAT ACTCACCCTGCACTCGCGATCCCTGAGCTGATGAGGATTTTTGTGGATATTGAAAAACTG CCCTGGTCCAAGGCATGGGAGCTCACCCAGAAGACCTTCGCCTACACCAACCACACAGTG CTCCCGGAAGCCCTGGAGCGCTGGCCCGTGGACCTGGTGGAGAAGCTGCTCCCTCGACAT TTGGAAATCATTTATGAGATAAATCAGAAGCATTTAGATAGAATTGTGGCCTTGTTTCCT AAAGATGTGGACCGTCTGAGAAGGATGTCTCTGATAGAAGAGGAAGGAAGCAAAAGGATC AACATGGCCCATCTCTGCATTGTCGGTTCCCATGCTGTGAATGGCGTGGCTAAAATCCAC TCAGACATCGTGAAGACTAAAGTATTCAAGGACTTCAGTGAGCTAGAACCTGACAAGTTT CAGAATAAAACCAATGGGATCACTCCAAGGCGCTGGCTCCTACTCTGCAACCCAGGACTT GCAGAGCTCATAGCAGAGAAAATTGGAGAAGACTATGTGAAAGACCTGAGCCAGCTGACG AAGCTCCACAGCTTCCTGGGTGATGATGTCTTCCTCCGGGAACTCGCCAAGGTGAAGCAG GAGAATAAGCTGAAGTTTTCTCAGTTCCTGGAGACGGAGTACAAAGTGAAGATCAACCCA TCCTCCATGTTTGATGTCCAGGTGAAGAGGATACATGAGTACAAGCGACAGCTCTTGAAC TGTCTGCATGTGATCACGATGTACAACCGCATTAAGAAAGACCCTAAGAAGTTATTCGTG CCAAGGACAGTTATCATTGGTGGTAAAGCTGCCCCAGGATATCACATGGCCAAAATGATC ATAAAGCTGATCACTTCAGTGGCAGATGTGGTGAACAATGACCCTATGGTTGGAAGCAAG TTGAAAGTCATCTTCTTGGAGAACTACAGAGTATCTCTTGCTGAAAAAGTCATTCCAGCC ACAGATCTGTCAGAGCAGATTTCCACTGCAGGCACCGAAGCCTCGGGGACAGGCAATATG AAGTTCATGCTAAATGGGGCCCTAACTATCGGGACCATGGATGGGGCCAATGTGGAAATG GCAGAAGAAGCTGGGGAAGAGAACCTGTTCATCTTTGGCATGAGGATAGATGATGTGGCT GCTTTGGACAAGAAAGGGTACGAGGCAAAAGAATACTATGAGGCACTTCCAGAGCTGAAG CTGGTCATTGATCAAATTGACAATGGCTTTTTTTCTCCCAAGCAGCCTGACCTCTTCAAA GATATCATCAACATGCTATTTTATCATGACAGGTTTAAAGTCTTTGCAGACTACGAAGCC TATGTCAAGTGTCAAGATAAAGTGAGTCAGCTGTACATGAATCCAAAGGCCTGGAACACA ATGGTACTCAAAAACATAGCTGCCTCGGGGAAATTCTCCAGTGACCGAACAATTAAAGAA TATGCCCAAAACATCTGGAACGTGGAACCTTCAGATCTAAAGATTTCTCTATCCAATGAA TCTAACAAAGTCAATGGAAATTGA Clone variation with respect to NM_002863.4 1020 c=>t >OriGene 5' read for NM_002863 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCAGCCCAGCGCCTCCG GCCGCACTTCCAGCTCTCTGCGCAGCCCGCCGCGCAGCCCGCCGCCCCAGCCATGGCGAA GCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGA GAACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTGGTCAAGGA CCGCAACGTGGCCACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTGCGCGACCA CCTGGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTACGACAAGTGCCCCAAGAGGGT ATATTACCTCTCTCTGGAATTTTACATGGGCCGAACATTACAGAACACCATGATCAACCT CGGTCTGCAAAATGCCTGTGATGAGGCCATTTACCAGCTTGGATTGGATATAGAAGAGTT AGAAGAAATTGAAGAAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTTGCTGCCTG CTTCTTGGATTCCATGGCAACCCTGNGACTTGCAGCCTATGGATACGGCATTCGGTATGA ATATGGGATTTTCAATCAGAAGATCCGAGATGGATGGCAGGTAGAAGAAGCAGATGATTG GCTCAGATATGGAAACCCTTGGGAGAAGTCCCGCCCAGAATTCATGCTGCCTGTGCACTT CTNATGGAAAGTAGAACACACCAACACCGGACCAAGTGATTGACACTCAAGTGGTCCTGG CTCTGCATATGACCCCCCGTGCCGGCTACATGATACACTGTCACACCTGCCCTCTGTCTG CTCGGCACCAATGACTTTACCTANAACCTTATGTTGAGACTCATCCAGCTGGCTGACCGA ACCTGCCGAGACACTNCCGGTCT >OriGene 3' read for NM_002863 unedited
CTATGGACCGGCGGCCCGCATTCTNANGATNCGNAGTTTTCTTTCTTTTTTCTTTTTTCT TTAGACCCTTGGAAAATTGACACTTTATTTTTAAGCTCTATTACATATAATTTCCCTCCC CATTCCCAGAGATACTCAACTATTATAGATTATTAGCTAACAAAACAAAAACCAGTGAAT GTTGTAAAAATGTTCAAGTTCAGTAAGAAGCTATGTTTTCTAGAGACATTGTAGAGTTCA ATTTCCATTGACTTTGTTAGATTCATTGGATAGAGAAATCTTTAGATCTGAAGGTTCCAC GTTCCAGATGTTTTGGGCATATTCTTTAATTGTTCGGTCACTGGAGAATTTCCCCGAGGC AGCTATGTTTTTGAGTACCATTGTGTTCCAGGCCTTTGGATTCATGTACAGCTGACTCAC TTTATCTTGACACTTGACATAGGCTTCGTAGTCTGCAAAGACTTTAAACCTGTCATGATA AAATAGCATGTTGATGATATCTTTGAAGAGGTCAGGCTGCTTGGGAGAAAAAAAGCCATT GTCAATTTGATCAATGACCAGCTTCAGCTCTGGAAGTGCCTCATAGTATTCTTTTGCCTC GTACCCTTTCTTGTCCAAAGCAGCCACATCATCTATCCTCATGCCAAAGATGAACAGGTT CTCTTCCCCAGCTTCTTCTGCCATTTCCACATTGGCCCCATCCATGGTCCCGATAGTTAG GGCCCCATTTAGCATGAACTTCATATTGCCTGTCCCCGAGGCTTCGGTGCCTGCAGTGGA AATCTGCTCTGACAGATCTGTGGCTGGAATGACCTTTTCAGCAAGAGAACTCTGTAGTTC TCCAAGAGATGACTTTCAACTTGCTTCCACCATAGGGTCATTGNTACCACATCTGCCACT GAGTGATAGCTTTAGATCATTTTGCCTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002863 |
| Insert Size | 2950 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002863.3, NP_002854.3 |
| RefSeq Size | 2830 bp |
| RefSeq ORF | 2544 bp |
| Locus ID | 5836 |
| UniProt ID | P06737 |
| Domains | phosphorylase |
| Protein Families | Druggable Genome |
| Protein Pathways | Insulin signaling pathway, Starch and sucrose metabolism |
| Gene Summary | This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210683 | PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 |
CNY 3,990.00 |
|
| RC210683L1 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210683L2 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210683L3 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210683L4 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210683 | PYGL (tGFP-tagged) - Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 |
CNY 4,370.00 |
