LCMT1 (BC029609) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029609 edited ACTGAGCCGCGCCAGCTGAGCCAGGTGGAGCCCTACTTTCCTCTCCTGCTTTCTGCCTGT GACTAGCGCCGTCCCCTACCGCCCGTCGACCCCACTTCCATGTCTGGCGGACACAGCTCC CCGGAACCTCCACGCCCATGGCCACTAGACAGAGGGAGTCTTCCTTCACCTCCTGCTTTT CCACCTGGAATTGCGACGCGGGCGACGAGGGCATGGGCTGCACCTGCGAAGATGCTTCCT TGTGCAAGAAGCGCCTGTCGGGCGCGGGATTCGGGGCTGGCATCTGGGACGCGGGCTGAG GTGGGAGGCGGGCCTGCATCTGAAGAATACGCTCGGAGGCTGGCAGGTTGCTGCCCCCGC CTCGCACGACCCTCGCTTCCCACCTGTGAAATGCACAGAACAGGGCTTCATTTATTTAAC GAATCGTTTCTGAGCTCCTGCTGTGAGCCAGGCTTGGAGCAAGCCTGGGTACTGTGGATG GGAGCAGGCCCCTGAACCAGGCGGACGGGGTCTTGGCGTTCTCGGAGCTAAACGCTGCTA GAAAGACAAACATGATGCACCTAACTACTGTCTCCGTAGATTATGAGGATGAGAGGTGGA GGTTCTCTCTCTTTTAGGTATGATCCCCGTTGTGCACCTGAAACTCAGGCGATGACTTTC AGTGAAGGAAATGACCTGTCTAGGGCCACATAATACCTGTTTGAAAAGCCAGTACCACAC TAAGTCTCCACTATTAAGGGTGTGATGGTGAGGGTGGGGTCGTAGGGAGCACAGGTATGT GTCAGAAAGAGAAAACATGAGGCTTGAGTGTCGTCCATTTATTCACTCATTCATTCTACA AAAATGGAGAGCTTACTTTGTGCCAGGGCCTTATTTGGGCTGGTTGGTGGGGATTCAGTA ATGGGAATACACCAAGAACAAGACAGACGTGCTGCCAGACATACTGGAACTCACGGTTTA GTTGGGAAAGACTGACAGGAAATAATTATCCATGAGATACATAATTAAAATTTGGTGTTG TGCACTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029609 unedited GGGCTCGNGTTTTGTATACGACTCACTATAGGGCGGCCGCGACTTCGCCCTTCCACGGAC CTGACCATGACCCTCCACACCAAAGGCATCTGGGGTGGCCCTACTGCATCAGATCCAAGG GAACGAGCTGGAGCCCCTGAACCGTCCGCAGCTCAAGATCCCCCTGGAGCGGCCCCTGGG CGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTA CGAGGTCAACGCCGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATGGATC AGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGACTGAGCCGCGCCAGCTGAGCCA GGTGGAGCCCTACTTTCCTCTCCTGCTTTCTGCCTGTGACTAGCGCCGTCCCCTACCGCC CGTCGACCCCACTTCCATGTCTGGCGGACACAGCTCCCCGGAACCTCCACGCCCATGGCC ACTAGACAGAGGGAGTCTTCCTTCACCTCCTGCTTTTCCACCTGGAATTGCGACGCGGGC GACGAGGGCATGGGCTGCACCTGCGAAGATGCTTCCTTGTGCAAGAAGCGCCTGTCGGGC GCGGGATTCGGGGCTGGCATCTGGGACGCCGGCTGAGGTGGGAGGCGGGCCTGCATCTGA AGATACGCTCGGAGGCTGGCAGGTTGCTGCCCCCGCCTGGCACGACCCTCGCTTCCACCT GTGAAATGCACAGAACAGGGCTTCATTTATTTAACCAATCGTTTCTGAGCTCCTGCTGTG AGCCAGGCCTGGGAGCAAGCCTGGGTACCGGGGGATGGGACCATGGCCCCTGAACCAGGC GTGAGGGGGTCTTGGCGTTCCTGGAAGCTAAACCCCTGTGGGAAGGCAAACATGGTGGCC CCCAACACTGGGTCCCTAAATATGAGGAGAAAA
Restriction Sites	Please inquire
ACCN	BC029609
Insert Size	1066 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC029609.1
RefSeq Size	1066 bp
Locus ID	51451
Protein Pathways	Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
Gene Summary	LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]

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