CHRNA6 (BC014456) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	acetylcholine receptor alpha 6 neural; Acra6; alpha6 nAChR; CHNRA6; cholinergic receptor, nicotinic, alpha polypeptide 6; Nica6; nicotinic acetylcholine receptor subunit alpha 6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014456 edited CCACAATCCTTTGCCTAGGAAAAAGGAATCCAAGTGTGTTTTAACCATGCTGACCAGCAA GGGGCAGGGATTCCTTCATGGGGGCTTGTGTCTCTGGCTGTGTGTGTTCACACCTTTCTT TAAAGGCTGTGTGGGCTGTGCAACTGAGGAGAGGCTCTTCCACAAACTGTTTTCTCATTA CAACCAGTTCATCAGGCCTGTGGAAAACGTTTCCGACCCTGTCACGGTACACTTTGAAGT GGCCATCACCCAGCTGGCCAACGTGGATGAAGTAAACCAGATCATGGAAACCAATTTGTG GCTGCGTCACATCTGGAATGATTATAAATTGCGCTGGGATCCAATGGAATATGATGGCAT TGAGACTCTTCGCGTTCCTGCAGATAAGATTTGGAAGCCCGACATTGTTCTCTATAACAA TGCTGTTGGTGACTTCCAAGTAGAAGGCAAAACAAAAGCTCTTCTTAAATACAATGGCAT GATAACCTGGACTCCACCAGCTATTTTTAAGAGTTCCTGCCCTATGGATATCACCTTTTT CCCTTTTGATCATCAAAACTGTTCCCTAAAATTTGGTTCCTGGACGTATGACAAAGCTGA AATTGATCTTCTAATCATTGGATCAAAAGTGGATATGAATGATTTTTGGGAAAACAGTGA ATGGGAAATCATTGATGCCTCTGGCTACAAACATGACATCAAATACAACTGTTGTGAAGA GATATACACAGATATAACCTATTCTTTCTACATTAGAAGATTGCCGATGTTTTACACGAT TAATCTGATCATCCCTTGTCTCTTTATTTCATTTCTAACCGTGTTGGTCTTTTACCTTCC TTCGGACTGTGGTGAAAAAGTGACGCTTTGTATTTCAGTCCTGCTTTCTCTGACTGTGTT TTTGCTGGTCATCACAGAAACCATCCCATCCACATCTCTGGTGGTCCCACTGGTGGGTGA GTACCTGCTGTTCACCATGATCTTTGTCACACTGTCCATCGTGGTGACTGTGTTTGTGTT GAACATACACTACCGCACCCCAACCACGCACACAATGCCCAGGTGGGTGAAGACAGTTTT CCTGAAGCTGCTGCCCCAGGTCCTGCTGATGAGGTGGCCTCTGGACAAGACAAGGGGCAC AGGCTCTGATGCAGTGCCCAGAGGCCTTGCCAGGAGGCCTGCCAAAGGCAAGCTTGCAAG CCATGGGGAACCCAGACATCTTAAAGAATGCTTCCATTGTCACAAATCAAATGAGCTTGC CACAAGCAAGAGAAGATTAAGTCATCAGCCATTACAGTGGGTGGTGGAAAATTCGGAGCA CTCGCCTGAAGTTGAAGATGTGATTAACAGTGTTCAGTTCATAGCAGAAAACATGAAGAG CCACAATGAAACCAAGGAGGTAGAAGATGACTGGAAATACGTGGCCATGGTGGTGGACAG AGTATTTCTTTGGGTATTTATAATTGTCTGTGTATTTGGAACTGCAGGGCTATTTCTACA GCCACTACTTGGGAACACAGGAAAATCTTAAAATGTATTTTCTTTTATGTTCAGAAATTT ACAGACACCATATTTGTTCTGCATTCCCTGCCACAAGGAAAGGAAAGCAAAGGCTTCCCA CCCAAGTCCCCCATCTGCTAAAACTGATGGCTACATGGAAAAAGAGGACTTTCTTGCACA TTAGGAAGCAGAATATCCTCCAGTGTAGCACAGGGGGAGAAGTGAGAAGTGTTATGACAT TAGTGCCCTGATTGGGCAGCACCCACGGCTACAGTATCAGGTCAGGTTTCTCGTGTCTGT GTGTTTGGCTTCTAAAGACAAGAATGACTTACAATACAGCCTAATTATGTAATTAACATC AAGCCACACTAAAAAGTTCTGCTGTACCTCCAGTGGATGCTGTTTCTCAGTATTATGACA TGTGACAGGTGAACTCTGGGAATTGGACCATGCAACATTTGTGAACTGTTTGCAACACTT TGTGAACTATTGCATCCTTGATTTCTTGTGCATGTCTCTGTAATGTAACTCACCCTCCTC AGCATTATTTGATACACTGATTGTTAGTTTTAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014456 unedited NNCCACTTCAACATTTGTNATACGACTTCACTATNAGGGACGGCCGCGNNAATTTCGGCA CGNAGGCCACNNAATCCTTTGCCTAGGNAAAAAGGAATCCAAGTGTGTTTTAACCATGCT GACCAGCAAGGGGCAGGGATTCCTTCATGGGGGCTTGTGTCTCTGGCTGTGTGTGTTCAC ACCTTTCTTTAAAGGCTGTGTGGGCTGTGCAACTGAGGAGAGGCTCTTCCACAAACTGTT TTCTCATTACAACCAGTTCATCAGGCCTGTGGAAAACGTTTCCGACCCTGTCACGGTACA CTTTGAAGTGGCCATCACCCAGCTGGCCAACGTGGATGAAGTAAACCAGATCATGGAAAC CAATTTGTGGCTGCGTCACATCTGGAATGATTATAAATTGCGCTGGGATCCAATGGAATA TGATGGCATTGAGACTCTTCGCGTTCCTGCAGATAAGATTTGGAAGCCCGACATTGTTCT CTATAACAATGCTGTTGGTGACTTCCAAGTAGAAGGCAAAACAAAAGCTCTTCTTAAATA CAATGGCATGATAACCTGGACTCCACCAGCTATTTTTAAGAGTTCCTGCCCTATGGATAT CACCTTTTTCCCTTTTGATCATCAAAACTGTTCCCTAAAATTTGGTTCCTGGACGTATGA CAAAGCTGAAATTGATCTTCTAATCATTGGATCAAAAGTGGATATGAATGATTTTTGGGA AAACAGTGAATGGGAAATCATTGATGCCTCTGGCTACAAACATGACATCAAATACAACTG TTGTGAAGAGATATACACAGATATAACCTATTCTTTCTACATTAGAAGATTGCCGATGTT TTACACGATTAATCTGATCATCCCTTGTCTCTTGATNTCATTTCTAAACCGTGTGGTCTT TTACCTTCC
Restriction Sites	Please inquire
ACCN	BC014456
Insert Size	2090 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC014456.1, AAH14456.1
RefSeq Size	2090 bp
RefSeq ORF	1482 bp
Locus ID	8973
Protein Families	Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Gene Summary	This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence. [provided by RefSeq, Dec 2010]

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