TPCN1 (AK093490) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TPC1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC124239 sequence for AK093490 edited (data generated by NextGen Sequencing) ATGGCTGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGT GCCCCACTGGCTCCTTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCAAAAATGGCGGC AGCTATGCCATCCACGACTCCCAGGCCCCCAGTCTCAGCTCTGGGGGTGAGAGTTCCCCC TCCAGCCCCGCACACAACTGGGAGATGAATTACCAAGAGGCAGCAATCTACCTCCAGGAA GGCGAGAACAACGACAAGTTCTTCACCCACCCCAAGGATGCCAAGGCGCTGGCGGCCTAC CTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCCACGGCCCTGCTGCTGCTG CTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATCTATGTCCAC GCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGC TGGCTGGGCCTCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTG GTGGTGCAGTTTGTCGAGGCCATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTG ACCCGAGCACTGCGCTGCATTTTCCTGGTGGACTGTCGGTATTGCGGTGGCGTCCGGCGC AACCTGCGGCAGATCTTCCAGTCCCTGCCGCCCTTCATGGACATCCTCCTGCTGCTGCTG TTCTTCATGATCATCTTTGCCATCCTCGGTTTCTACTTGTTCTCCCCTAACCCTTCAGAC CCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTCCTTCTGACCACAGCC AATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTC ATCGTGTACCTCTCCATTGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTC GACACCTTCAATGACATTGAGAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACC GCTATCCAGCATGCCTACCGCCTGCTCATCAGCCAGAGGAGGCCTGCCGGCATCTCCTAC AGGCAGTTTGAAGGCCTCATGCGCTTCTACAAGCCCCGGATGAGTGCCAGGGAGCGCTAT CTTACCTTCAAGGCCCTGAATCAGAACAACACACCCCTGCTCAGCCTAAAGGACTTTTAC GATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAGAAAAACAGAGAGCACTGGTTT GATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATCCTTGTGAAGTCC AAGGCCTTCCAGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTCGTG GAGACATTTATGCTGAAAGGTGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTC GTCTTTCTAACTATCTATGGGGTGGAGCTGTTCCTGAAGGTTGCCGGCCTGGGCCCTGTG GAGTACTTGTCTTCCGGATGGAACTTGTTTGACTTCTCCGTGACAGTGTTCGCCTTCCTG GGACTGCTGGCGCTGGCCCTCAACATGGAGCCCTTCTATTTCATCGTGGTCCTGCGCCCC CTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAGCGCTACCGCAACGTGCTGGACACCATG TTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTGCTCATCTTTTACTACTCC TTCGCCATCGTGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGCTGCAACACG AGTACAGTGGCAGATGCCTACCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTG GAGGAAGGCTACTATTATCTCAATAATTTTGACAACATCCTCAACAGCTTTGTGACCCTG TTTGAGCTCACAGTTGTCAACAACTGGTACATCATCATGGAAGGCGTCACCTCTCAGACC TCCCACTGGAGCCGCCTCTACTTCATGACCTTTTACATTGTGACCATGGTGGTGATGACG ATCATTGTCGCCTTTATCCTCGAGGCCTTCGTCTTCCGAATGAACTACAGCCGCAAGAAC CAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAGAAGGAAATCTCCAAAGAAGAGCTG GTTGCCGTCCTGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCGGATGTCACCAGGCTG CTGGAGACCCTCTCCCAGATGGAGAGATACCAGCAACATTCCATGGTGTTTCTGGGACGG CGATCAAGGACCAAGAGCGACCTGAGCCTGAAGATGTACCAGGAGGAGATCCAGGAGTGG TATGAGGAGCATGCCAGGGAGCAAGAGCAGCAGCGACAACTCAGCAGCAGTGCAGCCCCC GCCGCCCAGCAGCCCCCAGGCAGCCGCCAGCGCTCCCAGACCGTTACCTAG Clone variation with respect to AK093490.1 21 t=>c;75 c=>t;918 c=>t;1105 c=>t;1203 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093490 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCTGCCCTGGTGGCA GTGGCTGAAGTGGCGGCGGCTTCGGCGGCTGCGGCGGCTGCAACAGCTTCGGGCTCGGGG TTTTGGCGGCGGCGCCGGCGGGCTAGGCTGCGCGGTGCGGACCCCGGCGCGCGGTCCGGG TTGCTGGGGCGGCGCGTAGATGGAGTCTTGCTACATTGCCCAGGCTGGTCTTGAACTGCT AGGCTCAAGCAGTTCTCCCACCTTGACCTCCCAAAGCGCTGAGATTACAGAAGATGCCTC TAATGGAGGAGTTTCTGAGCAGCACCCCTGGCCCAGTGGCTTTGAAAGGGAGCTCAAACC AGAGACTATTTCAAGCCCTGGATATCATATCCTGAGGGCCACAGGAGAAGAGAACATGGC TGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGTGCCCC ACTGGCTCCTTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCANAAATGGCGGCAGCTA TGCCATTCACGACTNCCCAGCCCCCAGTCTCAGCTCTGGGGGTGAAGAGTTCCCCCTTCA GCCCCCGNCCAAAACTGGGGAAAGAAATTCCCAGAGGCCGCATTCTACCTCCCGAAGGGG AAAAACCCGACAGGTTTTTCTCCCCCCCCCAGGGAGCCAAGGGGCTTGGGGGCTCACCTT TTTGGACCAAAAAACCCTTTTTTACTTGGGGAAAATGGGCCCGGCCCCCGGGGGGGGGGG GGTCTCCCCCTTGGGGGGGGCCCCCGGGCCCCCCAAAATGGGGGGTGGGGTTTTTTTTCC CCCCCCCCCCCCTCACAANTTTTTCCCCTCCGGTGGGGGGGGGGTGTAACTCCCCTAAAA AAACCCCCCCGCCGCCGCCCA
Restriction Sites	NotI-NotI
ACCN	AK093490
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK093490.1, BAG52729.1
RefSeq Size	2817 bp
RefSeq ORF	2817 bp
Locus ID	53373
Domains	ion_trans
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
Gene Summary	Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008]

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