XRCC4 (BC005259) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNA repair protein XRCC4; X-ray repair, complementing defective, repair in Chinese hamster; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross complementing protein 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005259 edited AAACCTTGATCTGTGAAAGCGGGCGTTTTGGAAGATACCGGAAGTAGAGTCACGGAGAGG TATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCTGAACCCAGTATAACT CATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTTGTTATTACACTTACT GATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAGAGATTTCCCAAGAAGCTGAT GACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAAGCATTGTTGTCAGGA GCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCTTGTTATTTCTTCTTT GAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAACCTAGAGAAAGTTGAA AACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGACACCATTGCAGAAAAT CAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGCTTCTGAGAGATTGGAATGAT GTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTGGAGACTGATCTTTAT AAGCGGTTTATTCTGGTGTTGAATGAGAAGAAAACAAAAATCAGAAGTTTGCATAATAAA TTATTAAATGCAGCTCAAGAACGAGAAAAGGACATCAAACAAGAAGGGGAAACTGCAATC TGTTCTGAAATGACTGCTGACCGAGATCCAGTCTATGATGAGAGTACTGATGAGGAAAGT GAAAACCAAACTGATCTCTCTGGGTTGGCTTCAGCTGCTGTAAGTAAAGATGATTCCATT ATTTCAAGTCTTGATGTCACTGATATTGCACCAAGTAGAAAAAGGAGACAGCGAATGCAA AGAAATCTTGGGACAGAACCTAAAATGGCTCCTCAGGAGAATCAGCTTCAAGAAAAGGAA AAAGGGAGGAAGAAGGAGACTTCAGAGAAAGAGGCCGTGTGATGGAAGCAAAATCAGAGA GAAAAGATGCTAAGCTGCTGCCTTTGAATAAGGAGGAACGTGAGCCAACAGATCCAAGAA CTGAAGCTCTAGGCAGTGGAAAAGCCAAGGAAACTGATCTTCCCTTCGAGCCTCCGGAGG GAGTGTAGCCCTGCCAACACCTTTATTTTAGCCCCATAACAGTCATTTCAGACTTGTGGC CTCCAGAATTTTAAGAGAATAAATTTTTATTGTATTAAGCCATGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005259 unedited TAGGATTTTGTAATACGACTCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGGA AACCTTGATCTGTGAAAGCGGGGGTTTTGGAGGATACCGGAAGTAGAGTCACGGAGAGGT ATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCTGAACCCAGTATAACTC ATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTTGTTATTACACTTACTG ATGGGGATTCANCATGGACTGGGACAGTTTCTGAATCAGAGATTTCCCAAGAAGCTGATG ACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAAGCATTGTTGTCAGGAG CAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCTTGTTATTTCTTCTTTG AGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAACCTAGAGAAAGTTGAAA ACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGACCCATTGGCAGAAATCA AGCCAAAATGAGCACCTGCCAAAAGAAATGAAAGGCTTCTGAGAGATGGGATGATGTTCA AGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTGGAGACTGATCTTTATAAGCG GGTTATTCTGGGGGTGGATGAGAAGGAAACAAAAATCAGAGATGTGGTAATAAATTATTA AATGCGGCTCAGGAACGAGAAAGGGCCTCAAACAGGAGGGGAAACTGCCATCTGGTCCTG AATGACTGCTGGCCCAGATCCACTCCATTATGAGGGTCCTGTTAGAGAAATGGAAACCCA CCCGGTCCCTCTGGGGTGGGTTCCCCTGGGGAAACGAAAAAGAGTCCCTTTTTTCAAGCC TTGGGGCCCTTGATTTGGCCCACTGAAAAAGGGGCCCGCAATGCGCAAAACCTTGGGGCA AACCC
Restriction Sites	Please inquire
ACCN	BC005259
Insert Size	1300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005259.1, AAH05259.1
RefSeq Size	1274 bp
Locus ID	7518
Protein Families	Druggable Genome
Protein Pathways	Non-homologous end-joining
Gene Summary	The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]

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