United States
Germany
Japan
United Kingdom
China
GOT2 (NM_002080) Human Untagged Clone
CAT＃: SC127269
GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE82; KAT4; KATIV; KYAT4; mitAAT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127269 sequence for NM_002080 edited (data generated by NextGen Sequencing)
ATGGCCCTGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGC CTCGCCGCCGCGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCT CCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATG AATCTGGGAGTTGGTGCCTACCGGGATGATAACGGAAAGCCTTACGTGCTGCCTAGCGTC CGCAAGGCAGAGGCCCAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGG GGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAACAGCGAAGTC TTGAAGAGTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATC GGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCA ACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGG TATTATGACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAA ATACCAGAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGAC CCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGAATCTCTTTGCG TTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCT GTGCGCCACTTCATCGAACAGGGCATTAATGTTTGTCTCTGCCAATCATATGCCAAGAAC ATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAA GCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCC CTCAATGGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGG CTGCAAGAAGTGAAAGGCATGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCC AACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACATCACCGACCAAATTGGCATG TTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCATC TACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTAC CTTGCCCATGCCATTCACCAGGTCACCAAGTAA Clone variation with respect to NM_002080.2 213 t=>c;228 t=>g;816 c=>t;1037 t=>g >OriGene 5' read for NM_002080 unedited
GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCCCTGTGTGTGTGTC CGCTCGCCCTCTGCTCCGTCCTGCGGCTGCCCACTGCCCTCCTACGGTCCACCATGGCCC TGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGCCTCGCCG CCGCGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATC CCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGG GAGTTGGTGCCTACCGGGATGATAACGGAAAGCCTTACGTGCTGCCTAGCGTCCGCAAGG CAGAGGCCCAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGGGGACTGG CTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAACAGCGAAGTCTTGAAGA GTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCA GTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGG GAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATG ACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAAGATATTTCAAAATACCAGA GCAGAGTGTTCCTCTTCTGCATGCCTGCGCCCACAATCCCAGGGAGTGGACCCGCGTCCG GAAAGTGGGAGGAAATACCACCAGCGTGAAGAAAAGGAATCTCTTTGCGTNCTTTGACTT GCCCTACACAGCCTTGCCAGTGTGGATGGTGATAAGATGCCTGGGCTGGGCCCCACTCAT CG >OriGene 3' read for NM_002080 unedited
GGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGGTCTACAGTAATTTTATTGT AACAGAGAAACCAGGCCGCAATAAGTCTACATGGTTAGAGCAGATGGTGGTTCTTTCCAG TGGGTGAAGCCTGAACCCAGCTAGCAGCACGCAAAGCCTAGAATATCCTCTTTCCTGGTA CCACCCTGCGCCGCTGGACTCACAGTGTGAGATGAGAGGCTGCACACCACATTCAACAAA GGGAGGAGGTCCAACCGGGCAGAGACAACATCCCAACTGGAGAAACTTGCACTATCATTC AAGAGGATGCCGAGATAAAGTACAGTTTCCTACTCTCCACTATTCTTTGCTGAAAAGGGT GAGGGTACGGCATCAGCAGAAAATTATGTTCTTCATTCTTAAATAAATCTCGATAGGATG GAGTTGATGGTCAATCAAGCAGTAGAATGGGATGAAAATCTGCAATAGTTAAAAAATCTT TGCGTGTAAGGAAAAGAACGGCAATGACAAGGGAGAAAAGAGAGCCTTCTCTTTTTCTTA AATAAGACAGGAAACTGCATTATTTCCCTTGCTGGAGTGGCAGCAGCCTCATTGGTTTTC ATAAAACATCATTTCTGCAGTGCGGCCTGTTGATAAGGGTGTTCCAAGATGGGAGGACAG ACACGAGGCCGACTACTCATGACAGAACTAGGCCTTCTCTCAAAGCCTCTAACGTGTGCT GTTTCTCACGAGGAACCTGACACTTCAGNTAAAGCTTCTGAATATTGCAATGGCTATTGA CAGGTTTTTGTGCGATGACTTCTGCCATGTAACTCTTGAGAAAGTTGAGAAAAAGGACGC GAGAGTTCGGTTATATTCCAACGCACCCTGATGCCTTGCCTGGGACTACAGGTCTTTTGA AACATCAATGTGAAGGTAACCTGGGCTGAAGAAGATCATACCCCCATCACCTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002080 |
| Insert Size | 2410 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002080.2, NP_002071.2 |
| RefSeq Size | 2473 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 2806 |
| UniProt ID | P00505 |
| Domains | aminotran_1_2 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism |
| Gene Summary | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Targeted exome sequencing of Krebs cycle genes reveals candidate cancer predisposing mutations in pheochromocytomas and paragangliomas
,Remacha, L;Comino-Méndez, I;Richter, S;Contreras, L;Currás-Freixes, M;Pita, G;Letón, R;Galarreta, A;Torres-Pérez, R;Honrado, E;Jiménez, S;Maestre, L;Moran, S;Esteller, M;Satrústegui, J;Eisenhofer, G;Robledo, M;Cascon, A;,
Clin. Cancer Res.
,PubMed ID 28720665
[GOT2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201826 | GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC201826L3 | Lenti-ORF clone of GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC201826L4 | Lenti-ORF clone of GOT2 (mGFP-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RG201826 | GOT2 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 7,088.00 |
|
| SC320593 | GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
