PSMD4 (NM_153822) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_153822, the custom clone sequence may differ by one or more nucleotides ATGGTGTTGGAAAGCACTATGGTGTGTGTGGACAACAGTGAGTATATGCGGAATGGAGACTTCTTACCCA CCAGGCTGCAGGCCCAGCAGGATGCTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTGAGAA CAACGTGGGCCTTATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCACCCCAGACACTGGCCGT ATCCTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACCTTCTGCACGGGCATCCGCGTGGCCC ATCTGGCTCTGAAGCACCGACAAGGCAAGAATCACAAGATGCGCATCATTGCCTTTGTGGGAAGCCCAGT GGAGGACAATGAGAAGGATCTGGTGAAACTGGCTAAACGCCTCAAGAAGGAGAAAGTAAATGTTGACATT ATCAATTTTGGGGAAGAGGAGGTGAACACAGAAAAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAG ATGGAACCGGTTCTCATCTGGTGACAGTGCCTCCTGGGCCCAGTTTGGCTGATGCTCTCATCAGTTCTCC GATTTTGGCTGGTGAAGGTGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAATTTGGAGTAGATCCC AGTGCTGATCCTGAGCTGGCCTTGGCCCTTCGTGTATCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAGG AGGCCCGGCGGGCAGCTGCAGCTTCTGCTGCTGAGGCCGGGATTGCTACGACTGGGACTGAAGGTGAAAG AGGTGGAATCCGAAGTCCTGGGACTGCGGGATGCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_153822 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTCTCTCTCTCTCTCAC CCGGTCGGGAGGGAGGAAGGTGGCAAGATGGTGTTGGAAAGCACTATGGTGTGTGTGGAC AACAGTGAGTATATGCGGAATGGAGACTTCTTACCCACCAGGCTGCAGGCCCAGCAGGAT GCTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTGAGAACAACGTGGGCCTT ATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCACCCCAGACACTGGCCGTATC CTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACCTTCTGCACGGGCATCCGC GTGGCCCATCTGGCTCTGAAGCACCGACAAGGCAAGAATCACAAGATGCGCATCATTGCC TTTGTGGGAAGCCCAGTGGAGGACAATGAGAAGGATCTGGTGAAACTGGCTAAACGCCTC AAGAAGGAGAAAGTAAATGTTGACATTATCAATTTTGGGGAAGAGGAGGTGAACACAGAA AAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAGATGGAACCGGTTCTCATCTGGTG ACAGTGCCTCCTGGGCCCAGTTTGGCTGATGCTCTCATCAGTTCTCCCGATTTTGCCTGG TGAAGGTGGTGCCATGCCTGGTCTTGCTGCCAGTGACCTTTGAATTTGGAGTAGATCCCA GTGCTGATCCTGAACTGGCCCTTGGCCCTTCTGGTTTCTATGAACACCANCGGCACCGGC AAGAAGAGGAGGCCCGGCTGCCAGTTGTACTTTTGCTGCTGAAGCCCGGACTGCTACCAC TGGGACTGAAGTGAAAAAGCACCACACAATGCCCTGCTGAAGATGACCATCACCCCCAAG ATTTGCGCGCCTGGGCTTTCTGACCAAACAAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_153822 unedited GGCAGCTATACGCAGTTTGTAATGGTGACAGCAAACACACATNCTAACCCTATATTCCGT GTGTCCCATGCGTCCCCTAAGCAGACTGAGCTACCCTTTCCCTCCAGTCTCACTTCTTGT CTTCCTCCTTCTTGTCCTTCTTGCCGTCCTTGGTGGCCTGGGAGGCCAGGGAGCCCATAC CATTTCGAATGGCTTCATTGTTGGGATCCACACCTGGGAGGTCCTCTAGGACACTCTGAG GGAGACTCGGGGTCCTGCATCACGTCGTAATCATCCTCCTCCTTGGCTGGCTCGGATGTG TCCATAGCTGAGCTGGCATCAATGTCTGCTGATTCCGACTGGCCAAACTCTGCTCCCTGC AGGGACATCTGCATGGCATAAGCAATCTGCTCTTCCTCAGCCATACTGCTTAGGTCAGGA AGCCCAGTGCGGCCAAACTCTTGCTGGCTGATGGTCATCTTCAGCAGGGCATCGGCTGAG TCTCTTTCACCTTCATTCCCAGTCGTATCAATACCGGCCTCACCACCATAAGCTGTACCT GCCCGCCGGGCCTACTCCTACTGCCGNTGACGGTGCTCTTTCATACATACACGAATGGCC AACGCCAGCTCAGGATCAGCACTGAGTATCTACTCCAAATTCAAAGTCACTGGCACCAAG ACCCACCATGGCCACCACCTTCACCACGCGAATTCGGAGAACTGATGATAGCATCAACCA GCACGGGCCCACGAGGTACTTTCACAGATGAGATCCGGGTCCCATCTTGCCTTTAACCTT TTACCAAGGCTCTCAGCTTCTCCTGGTTAACCTCCCTTTCCCCAATGGGAAAGGCAACCT CACTATCCCCTCTTCAGCGTATACCGTCGTCGACACCTGTCCATGTCCCCCTCGGCTCCC CCATGCCCGAGACCTCCTGGCAGTTGCCTTGCGCCCTCCACCCAGGGCCCTCGATCCGTG CTAAG
Restriction Sites	NotI-NotI
ACCN	NM_153822
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153822.1, NP_722544.1
RefSeq Size	1527 bp
RefSeq ORF	1527 bp
Locus ID	5710
Protein Pathways	Proteasome
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2) has a distinct C-terminus compared to isoform 1.

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