PI 3 Kinase Class 3 (PIK3C3) (AK022653) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hVps34; VPS34; Vps34
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK022653, the custom clone sequence may differ by one or more nucleotides TTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCATGGACAA GCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTACAAAACAT GGCTTCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACT TTTTTAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGGTCATGGACACTTACGT TAAAAGCTGTGCTGGATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGATAACCTT TTGCTAACAAAAACAGGTAACAATTAATGACTACCAGTAGACATACATTGTATATGTCCATGGTTTTTAC CCCTGAATCTATGTACTAACAAGATAAGTTGCGGCCTGGCGCGGTGGCTCACGCCCGTAATCCCAGCACT TTGGGAGGCCAAGACGGGTGGATCACCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAAC CCCGTCTCTACTAAACACACAAAATTTGCCGGGCGTGGTGGTACATGCCTGTAATCCCAACTACTCAGGA GGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCACCATTGCAC TGCAGACTAGGCAACAAGATCAAAACGCCATCTTAGAAAAAAAAAAAAAAAAAAGATGAGTTGCGTAATT GAAGATAAATCAGAGAGTCTTAAAAATTCCTTTAAATGTTACCTTGGAAGAACATTTAGGGAAAGTCCTT TCCCTTTTAAGAATTGCTTTAGTAGTAATCCTACAGATGGTCATTTTGCCCACACTTCTGTACTGGAAAA TGCCTCTTTTTATGGAAAGCCAATAATGGATACAAGTAGCTTAGTCTCTTACTGTATAGCTCTAGTTAGA TTGTTAAAATGTCCTTTCTCTTACAAAGTGTGCTTAATTCACTTTAATATCTACCTTTAATTATTTGCTT TTCCCCTTGGAGTTCTATGGGATAATCATTCTCAGTCCCCTTTGTCCTGACAACTCTTTAGCCCTATGTA TTTATCTTTATGCAGACAGCTTCCAGACTTACAGCTTTTTACCTCAGTCCATTTCATTAACTTCAAATTG AAATATCGAACTGCTTACTTGAAATTGTCATTATATATGGCCAGTTGTAAAAGGTTGTCCTTTCCTCTAC TACCTGCCTAAAACCTGTTTCTCACCTCATTTTTCCATTTCAATAGATAGCAACATCACCTACCCAGTTG TTCAAGCTCTAGACCTCAAAGTCATTCTTAACATGTCTCTTGGCCTCATCTCATATACTGAACCTATTAG CAAATCTTGTCTACTCCTCAACTCCAAAATAGATCATGAAGTTACCCACTGTCTTCATTTTTACTACTTT GACCCCACTTGAAGCAAACAATATATGTTGCCTAAACAATTTGAATGAAACCTGAACCCTTTATTGGGGC CTTGTGGCTTGTCCAGGCAGGCCATTTGTCTGAATGTCAAACCACTCCTGCCTCAGACAAGAGCCTTTGT TGTTTTCTCTTTCTGGAAAGTTATTTATTCAGATCTTTGCAGGGCTGACTTTAGTTATTAAGTTGAAGTT CTAATGGCATCTCTTTAGAGAAGCCCTTTCTAACTCCTTGATCTGTAATAACATATCTGCCCTCTTCCTC CAATGCTCTCTACTTATTACTGTATTGTTTATAGCACTTATCACTAGCTGAAGTTACCTTTTTTGTTTAA TTCAACAACTATTTATTGAACAGCTAGTAGATGCTGGGAATACCAAAGTTAACAGAAGAGACAGAAATCC TTGCTCTCCTAGAGCTTGTTGAGTTCTTATCATCATCTTCTCCCCTTCAGGATGCAAGTTCCATGAGAGC AGGAACCTTTGTCCATTGGGGTTACCCCCATGCCTCTGGACCTTGAACACCACCTAGCTAAAGTGTTCAA TAAATCAGTGTTGAATTAATGATGAATAAATACTTCTGCTCTTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK022653 unedited GTACCAAGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTTAAAAGGCGTTTTGAT CTTGTTGCCTAGTCTGCAGTGCAATGGTGCAATCTCGGCTCACTGCAACCTCCGCCTCCC GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGTTGGGATTACAGGCATGTACCA CCACGCCCGGCAAATTTTGTGTGTTTATTTTATACGGGGTTTCTCCATGTTGGTCAGGCT GGTCTCGAACTCCCAACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATT ACGGGCGTGAGCCACCGCGCCAGGCCGCAACTTATCTTGTTAGTACATAGATTCAGGGGT AAAAACCATGGGCATATACAATGTATGTCTACTGGTAGTCATTAATTGTTACCTGTTTTT GTTAGCAAAAGGTTATCCAGGTGCCTGTCTCCAACTCCAAGTATATAGGTGATCACGCAA TATCCAGCACAGCTTTTAACGTAAGTGTCCATGACCTCAGCACTAATCCCATTTGGCCCA TTCTCACTTGGTGCATATTTTCTAAAAAAGTTCTGAATGCTTCCCTCTGTATCAAGAACT TCAGCCACAGGAACTGACTGGATAAACTGCATGAAGCCATGTTTTGTACTGGTGGCTAAC ACCTTATAAGGTGTCAATTTCAAGTCCAGATTTTCTTTCCGTAACACCTTGTCCATGAGT GAAATGATTTGAAGAATAAGTTGATCTTGACGTAAATCATCTCCATGCTTAAATATAACT GGATATTTGCCTCCATCTTCCGTCTTANAAAACAACTGTGCAGGCATAAAGGCACTCTTA AACAGTGTAGCTTGTTCCCGGAATATTCCTCTAATTNTCACTTGGGGTTTCTAAAGGCAA CGGGATAAGTCCCACATCTGACAAATTATCTTT
Restriction Sites	NotI-NotI
ACCN	AK022653
Insert Size	2840 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK022653.1
RefSeq Size	2074 bp
RefSeq ORF	2074 bp
Locus ID	5289
Domains	PI3_PI4_kinase
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy
Gene Summary	Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.