Thyroglobulin (TG) (NM_003235) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AITD3; TGN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303286 representing NM_003235. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTGGTCCTGGAGATCTTCACCCTGCTGGCCTCCATCTGCTGGGTGTCGGCCAATATCTTCGAG TACCAGGTGGATGCCCAGCCCCTTCGTCCCTGTGAGCTGCAGAGGGAAACGGCCTTTCTGAAGCAAGCA GACTACGTGCCCCAGTGTGCAGAGGATGGCAGCTTCCAGACTGTCCAGTGCCAGAACGACGGCCGCTCC TGCTGGTGTGTGGGTGCCAACGGCAGTGAAGTGCTGGGCAGCAGGCAGCCAGGACGGCCTGTGGCTTGT CTGTCATTTTGTCAGCTACAGAAACAGCAGATCTTACTGAGTGGCTACATTAACAGCACAGACACCTCC TACCTCCCTCAGTGTCAGGATTCAGGGGACTACGCGCCTGTTCAGTGTGATGTGCAGCAGGTCCAGTGC TGGTGTGTGGACGCAGAGGGGATGGAGGTGTATGGGACCCGCCAGCTGGGGAGGCCAAAGCGATGTCCA AGGAGCTGTGAAATAAGAAATCGTCGTCTTCTCCACGGGGTGGGAGATAAGTCACCACCCCAGTGTTCT GCGGAGGGAGAGTTTATGCCTGTCCAGTGCAAATTTGTCAACACCACAGACATGATGATTTTTGATCTG GTCCACAGCTACAACAGGTTTCCAGATGCATTTGTGACCTTCAGTTCCTTCCAGAGGAGGTTCCCTGAG GTATCTGGGTATTGCCACTGTGCTGACAGCCAAGGGCGGGAACTGGCTGAGACAGGTTTGGAGTTGTTA CTGGATGAAATTTATGACACCATTTTTGCTGGCCTGGACCTTCCTTCCACCTTCACTGAAACCACCCTG TACCGGATACTGCAGAGACGGTTCCTCGCAGTTCAATCAGTCATCTCTGGCAGATTCCGATGCCCCACA AAATGTGAAGTGGAGCGGTTTACAGCAACCAGCTTTGGTCACCCCTATGTTCCAAGCTGCCGCCGAAAT GGCGACTATCAGGCGGTGCAGTGCCAGACGGAAGGGCCCTGCTGGTGTGTGGACGCCCAGGGGAAGGAA ATGCATGGAACCCGGCAGCAAGGGGAGCCGCCATCTTGTGCTGAAGGCCAATCTTGTGCCTCCGAAAGG CAGCAGGCCTTGTCCAGACTCTACTTTGGGACCTCAGGCTACTTCAGCCAGCACGACCTGTTCTCTTCC CCAGAGAAAAGATGGGCCTCTCCAAGAGTAGCCAGATTTGCCACATCCTGCCCACCCACGATCAAGGAG CTCTTTGTGGACTCTGGGCTTCTCCGCCCAATGGTGGAGGGACAGAGCCAACAGTTTTCTGTCTCAGAA AATCTTCTCAAAGAAGCCATCCGAGCAATTTTTCCCTCCCGAGGGCTGGCTCGTCTTGCCCTTCAGTTT ACCACCAACCCAAAGAGACTCCAGCAAAACCTTTTTGGAGGGAAATTTTTGGTGAATGTTGGCCAGTTT AACTTGTCTGGAGCCCTTGGCACAAGAGGCACATTTAACTTCAGTCAATTTTTCCAGCAACTTGGTCTT GCAAGCTTCTTGAATGGAGGGAGACAAGAAGATTTGGCCAAGCCACTCTCTGTGGGATTAGATTCAAAT TCTTCCACAGGAACCCCTGAAGCTGCTAAGAAGGATGGTACTATGAATAAGCCAACTGTGGGCAGCTTT GGCTTTGAAATTAACCTACAAGAGAACCAAAATGCCCTCAAATTCCTTGCTTCTCTCCTGGAGCTTCCA GAATTCCTTCTCTTCTTGCAACATGCTATCTCTGTGCCAGAAGATGTGGCAAGAGATTTAGGTGATGTG ATGGAAACGGTACTCAGCTCCCAGACCTGTGAGCAGACACCTGAAAGGCTATTTGTCCCATCATGCACG ACAGAAGGAAGCTATGAGGATGTCCAATGCTTTTCCGGAGAGTGCTGGTGTGTGAATTCCTGGGGCAAA GAGCTTCCAGGCTCAAGAGTCAGAGGTGGACAGCCAAGGTGCCCCACAGACTGTGAAAAGCAAAGGGCT CGCATGCAAAGCCTCATGGGCAGCCAGCCTGCTGGCTCCACCTTGTTTGTCCCTGCTTGTACTAGTGAG GGACATTTCCTGCCTGTCCAGTGCTTCAACTCAGAGTGCTACTGTGTTGATGCTGAGGGTCAGGCCATT CCTGGAACTCGAAGTGCAATAGGGAAGCCCAAGAAATGCCCCACGCCCTGTCAATTACAGTCTGAGCAA GCTTTCCTCAGGACGGTGCAGGCCCTGCTCTCTAACTCCAGCATGCTACCCACCCTTTCCGACACCTAC ATCCCACAGTGCAGCACCGATGGGCAGTGGAGACAAGTGCAATGCAATGGGCCTCCTGAGCAGGTCTTC GAGTTGTACCAACGATGGGAGGCTCAGAACAAGGGCCAGGATCTGACGCCTGCCAAGCTGCTAGTGAAG ATCATGAGCTACAGAGAAGCAGCTTCCGGAAACTTCAGTCTCTTTATTCAAAGTCTGTATGAGGCTGGC CAGCAAGATGTCTTCCCGGTGCTGTCACAATACCCTTCTCTGCAAGATGTCCCACTAGCAGCACTGGAA GGGAAACGGCCCCAGCCCAGGGAGAATATCCTCCTGGAGCCCTACCTCTTCTGGCAGATCTTAAATGGC CAACTCAGCCAATACCCGGGGTCCTACTCAGACTTCAGCACTCCTTTGGCACATTTTGATCTTCGGAAC TGCTGGTGTGTGGATGAGGCTGGCCAAGAACTGGAAGGAATGCGGTCTGAGCCAAGCAAGCTCCCAACA TGTCCTGGCTCCTGTGAGGAAGCAAAGCTCCGTGTACTGCAGTTCATTAGGGAAACGGAAGAGATTGTT TCAGCTTCCAACAGTTCTCGGTTCCCTCTGGGGGAGAGTTTCCTGGTGGCCAAGGGAATCCGGCTGAGG AATGAGGACCTCGGCCTTCCTCCGCTCTTCCCGCCCCGGGAGGCTTTCGCGGAGCAGTTTCTGCGTGGG AGTGATTACGCCATTCGCCTGGCGGCTCAGTCTACCTTAAGCTTCTATCAGAGACGCCGCTTTTCCCCG GACGACTCGGCTGGAGCATCCGCCCTTCTGCGGTCGGGCCCCTACATGCCACAGTGTGATGCGTTTGGA AGTTGGGAGCCTGTGCAGTGCCACGCTGGGACTGGGCACTGCTGGTGTGTAGATGAGAAAGGAGGGTTC ATCCCTGGCTCACTGACTGCCCGCTCTCTGCAGATTCCACAGTGCCCGACAACCTGCGAGAAATCTCGA ACCAGTGGGCTGCTTTCCAGTTGGAAACAGGCTAGATCCCAAGAAAACCCATCTCCAAAAGACCTGTTC GTCCCAGCCTGCCTAGAAACAGGAGAGTATGCCAGGCTGCAGGCATCGGGGGCTGGCACCTGGTGTGTG GACCCTGCATCAGGAGAAGAGTTGCGGCCTGGCTCGAGCAGCAGTGCCCAGTGCCCAAGCCTCTGCAAT GTGCTCAAGAGTGGAGTCCTCTCCAGGAGAGTCAGCCCAGGCTATGTCCCAGCCTGCAGGGCAGAGGAT GGGGGCTTTTCCCCAGTGCAATGTGACCAGGCCCAGGGCAGCTGCTGGTGTGTCATGGACAGCGGAGAA GAGGTGCCTGGGACGCGCGTGACCGGGGGCCAGCCCGCCTGTGAGAGCCCGCGGTGTCCGCTGCCATTC AACGCGTCGGAGGTGGTTGGTGGAACAATCCTGTGTGAGACAATCTCGGGCCCCACAGGCTCTGCCATG CAGCAGTGCCAATTGCTGTGCCGCCAGGGCTCCTGGAGCGTGTTTCCACCAGGGCCATTGATATGTAGC CTGGAGAGCGGACGCTGGGAGTCACAGCTGCCTCAGCCCCGGGCCTGCCAACGGCCCCAGCTGTGGCAG ACCATCCAGACCCAAGGGCACTTTCAGCTCCAGCTCCCGCCGGGCAAGATGTGCAGTGCTGACTACGCG GATTTGCTGCAGACTTTCCAGGTTTTCATATTGGATGAGCTGACAGCCCGCGGCTTCTGCCAGATCCAG GTGAAGACTTTTGGCACCCTGGTTTCCATTCCTGTCTGCAACAACTCCTCTGTGCAGGTGGGTTGTCTG ACCAGGGAGCGTTTAGGAGTGAATGTTACATGGAAATCACGGCTTGAGGACATCCCAGTGGCTTCTCTT CCTGACTTACATGACATTGAGAGAGCCTTGGTGGGCAAGGATCTCCTTGGGCGCTTCACAGATCTGATC CAGAGTGGCTCATTCCAGCTTCATCTGGACTCCAAGACGTTCCCAGCGGAAACCATCCGCTTCCTCCAA GGGGACCACTTTGGCACCTCTCCCAGGACATGGTTTGGGTGCTCGGAAGGATTCTACCAAGTCTTGACA AGTGAGGCCAGTCAGGACGGACTGGGATGCGTTAAGTGTCCTGAAGGAAGCTATTCCCAAGATGAGGAA TGCATTCCTTGTCCTGTTGGATTCTACCAAGAACAGGCAGGGAGCTTGGCCTGTGTCCCATGTCCTGTG GGCAGAACGACCATTTCTGCTGGAGCTTTCAGCCAGACTCACTGTGTCACTGACTGTCAGAGGAACGAA GCAGGCCTGCAATGTGACCAGAATGGCCAGTATCGAGCCAGCCAGAAGGACAGGGGCAGTGGGAAGGCC TTCTGTGTGGACGGCGAGGGGCGGAGGCTGCCATGGTGGGAAACAGAGGCCCCTCTTGAGGACTCACAG TGTTTGATGATGCAGAAGTTTGAGAAGGTTCCAGAATCAAAGGTGATCTTCGACGCCAATGCTCCTGTG GCTGTCAGATCCAAAGTTCCTGATTCTGAGTTCCCCGTGATGCAGTGCTTGACAGATTGCACAGAGGAC GAGGCCTGCAGCTTCTTCACCGTGTCCACGACGGAGCCAGAGATTTCCTGTGATTTCTATGCTTGGACA AGTGACAATGTTGCCTGCATGACTTCTGACCAGAAACGAGATGCACTGGGGAACTCAAAGGCCACCAGC TTTGGAAGTCTTCGCTGCCAGGTGAAAGTGAGGAGCCATGGTCAAGATTCTCCAGCTGTGTATTTGAAA AAGGGCCAAGGATCCACCACAACACTTCAGAAACGCTTTGAACCCACTGGTTTCCAAAACATGCTTTCT GGATTGTACAACCCCATTGTGTTCTCAGCCTCAGGAGCCAATCTAACCGATGCTCACCTCTTCTGTCTT CTTGCATGCGACCGTGATCTGTGTTGCGATGGCTTCGTCCTCACACAGGTTCAAGGAGGTGCCATCATC TGTGGGTTGCTGAGCTCACCCAGTGTCCTGCTTTGTAATGTCAAAGACTGGATGGATCCCTCTGAAGCC TGGGCTAATGCTACATGTCCTGGTGTGACATATGACCAGGAGAGCCACCAGGTGATATTGCGTCTTGGA GACCAGGAGTTCATCAAGAGTCTGACACCCTTAGAAGGAACTCAAGACACCTTTACCAATTTTCAGCAG GTTTATCTCTGGAAAGATTCTGACATGGGGTCTCGGCCTGAGTCTATGGGATGTAGAAAAGACACAGTG CCAAGGCCAGCATCTCCAACAGAAGCAGGTTTGACAACAGAACTTTTCTCCCCTGTGGACCTCAACCAG GTCATTGTCAATGGAAATCAATCACTATCCAGCCAGAAGCACTGGCTTTTCAAGCACCTGTTTTCAGCC CAGCAGGCAAACCTATGGTGCCTTTCTCGTTGTGTGCAGGAGCACTCTTTCTGTCAGCTCGCAGAGATA ACAGAGAGTGCATCCTTGTACTTCACCTGCACCCTCTACCCAGAGGCACAGGTGTGTGATGACATCATG GAGTCCAATGCCCAGGGCTGCAGACTGATCCTGCCTCAGATGCCAAAGGCCCTGTTCCGGAAGAAAGTT ATACTGGAAGATAAAGTGAAGAACTTTTACACTCGCCTGCCGTTCCAAAAACTGATGGGGATATCCATT AGAAATAAAGTGCCCATGTCTGAAAAATCTATTTCTAATGGGTTCTTTGAATGTGAACGACGGTGCGAT GCGGACCCATGCTGCACTGGCTTTGGATTTCTAAATGTTTCCCAGTTAAAAGGAGGAGAGGTGACATGT CTCACTCTGAACAGCTTGGGAATTCAGATGTGCAGTGAGGAGAATGGAGGAGCCTGGCGCATTTTGGAC TGTGGCTCTCCTGACATTGAAGTCCACACCTATCCCTTCGGATGGTACCAGAAGCCCATTGCTCAAAAT AATGCTCCCAGTTTTTGCCCTTTGGTTGTTCTGCCTTCCCTCACAGAGAAAGTGTCTCTGGACTCGTGG CAGTCCCTGGCCCTCTCTTCAGTGGTTGTTGATCCATCCATTAGGCACTTTGATGTTGCCCATGTCAGC ACTGCTGCCACCAGCAATTTCTCTGCTGTCCGAGACCTCTGTTTGTCGGAATGTTCCCAACATGAGGCC TGTCTCATCACCACTCTGCAAACCCAACCTGGGGCTGTGAGATGTATGTTCTATGCTGATACTCAAAGC TGCACACATAGTCTGCAGGGTCAGAACTGCCGACTTCTGCTTCGTGAAGAGGCCACCCACATCTACCGG AAGCCAGGAATCTCTCTGCTCAGCTATGAGGCATCTGTACCTTCTGTGCCCATTTCCACCCATGGCCGG CTGCTGGGCAGGTCCCAGGCCATCCAGGTGGGTACCTCATGGAAGCAAGTGGACCAGTTCCTTGGAGTT CCATATGCTGCCCCGCCCCTGGCAGAGAGGCGCTTCCAGGCACCAGAGCCCTTGAACTGGACAGGCTCC TGGGATGCCAGCAAGCCAAGGGCCAGCTGCTGGCAGCCAGGCACCAGAACATCCACGTCTCCTGGAGTC AGTGAAGATTGTTTGTATCTCAATGTGTTCATCCCTCAGAATGTGGCCCCTAACGCGTCTGTGCTGGTG TTCTTCCACAACACCATGGACAGGGAGGAGAGTGAAGGATGGCCGGCTATCGACGGCTCCTTCTTGGCT GCTGTTGGCAACCTCATCGTGGTCACTGCCAGCTACCGAGTGGGTGTCTTCGGCTTCCTGAGTTCTGGG TCCGGAGAGGTGAGTGGCAACTGGGGGCTGCTGGACCAGGTGGCGGCTCTGACCTGGGTGCAGACCCAC ATCCGAGGATTTGGCGGGGACCCTCGGCGCGTGTCCCTGGCAGCAGACCGTGGCGGGGCTGATGTGGCC AGCATCCACCTTCTCACGGCCAGGGCCACCAACTCCCAACTTTTCCGGAGAGCTGTGCTGATGGGAGGC TCCGCACTCTCCCCGGCCGCCGTCATCAGCCATGAGAGGGCTCAGCAGCAGGCAATTGCTTTGGCAAAG GAGGTCAGTTGCCCCATGTCATCCAGCCAAGAAGTGGTGTCCTGCCTCCGCCAGAAGCCTGCCAATGTC CTCAATGATGCCCAGACCAAGCTCCTGGCCGTGAGTGGCCCTTTCCACTACTGGGGTCCTGTGATCGAT GGCCACTTCCTCCGTGAGCCTCCAGCCAGAGCACTGAAGAGGTCTTTATGGGTAGAGGTCGATCTGCTC ATTGGGAGTTCTCAGGACGACGGGCTCATCAACAGAGCAAAGGCTGTGAAGCAATTTGAGGAAAGTCGA GGCCGGACCAGTAGCAAAACAGCCTTTTACCAGGCACTGCAGAATTCTCTGGGTGGCGAGGACTCAGAT GCCCGCGTCGAGGCTGCTGCTACATGGTATTACTCTCTGGAGCACTCCACGGATGACTATGCCTCCTTC TCCCGGGCTCTGGAGAATGCCACCCGGGACTACTTTATCATCTGCCCTATAATCGACATGGCCAGTGCC TGGGCAAAGAGGGCCCGAGGAAACGTCTTCATGTACCATGCTCCTGAAAACTACGGCCATGGCAGCCTG GAGCTGCTGGCGGATGTTCAGTTTGCCTTGGGGCTTCCCTTCTACCCAGCCTACGAGGGGCAGTTTTCT CTGGAGGAGAAGAGCCTGTCGCTGAAAATCATGCAGTACTTTTCCCACTTCATCAGATCAGGAAATCCC AACTACCCTTATGAGTTCTCACGGAAAGTACCCACATTTGCAACCCCCTGGCCTGACTTTGTACCCCGT GCTGGTGGAGAGAACTACAAGGAGTTCAGTGAGCTGCTCCCCAATCGACAGGGCCTGAAGAAAGCCGAC TGCTCCTTCTGGTCCAAGTACATCTCGTCTCTGAAGACATCTGCAGATGGAGCCAAGGGCGGGCAGTCA GCAGAGAGTGAAGAGGAGGAGTTGACGGCTGGATCTGGGCTAAGAGAAGATCTCCTAAGCCTCCAGGAA CCAGGCTCTAAGACCTACAGCAAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_003235
Insert Size	8307 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003235.4
RefSeq Size	8453 bp
RefSeq ORF	8307 bp
Locus ID	7038
UniProt ID	P01266
Protein Families	Druggable Genome
Protein Pathways	Autoimmune thyroid disease
MW	304.8 kDa
Gene Summary	Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

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