PRPF40B (NM_012272) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HYPC
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_012272, the custom clone sequence may differ by one or more nucleotides ATGCCCCCTCCAGGGATCCCCCCACCCTTTCCTCCGATGGGGCTACCCCCCATGAGTCAG AGACCACCAGCTATCCCCCCCATGCCACCTGGCATCCTGCCCCCAATGCTTCCACCAATG GGGGCGCCACCACCACTCACACAGATACCAGGAATGGTACCTCCGATGATGCCAGGAATG CTGATGCCAGCGGTGCCTGTCACCGCAGCGACGGCTCCGGGTGCGGACACCGCCAGCTCT GCTGTGGCTGGGACAGGCCCTCCGAGGGCCCTATGGAGTGAGCATGTGGCCCCAGATGGG CGCATCTACTACTACAATGCTGACGACAAGCAGTCCGTGTGGGAGAAGCCCAGCGTGCTC AAGTCCAAGGCAGAGCTGCTCCTGTCCCAATGTCCCTGGAAAGAGTACAAGTCGGACACA GGCAAACCTTATTACTATAACAACCAGAGTAAAGAGTCCCGCTGGACCCGGCCCAAGGAT CTGGATGACCTAGAGGTTCTAGTCAAACAAGAGGCTGCAGGGAAACAGCAGCAGCAGCTG CCACAGACACTTCAGCCACAGCCACCTCAGCCACAGCCTGACCCCCCACCTGTGCCTCCT GGCCCCACCCCAGTGCCCACAGGCCTCCTGGAACCTGAGCCAGGTGGGAGTGAAGATTGT GATGTGTTGGAGGCCACCCAGCCCCTGGAACAGGGGTTCCTGCAGCAGCTGGAGGAGGGC CCCAGCAGTTCTGGACAGCATCAGCCACAGCAGGAGGAGGAGGAATCAAAGCCAGAACCA GAGAGGTCTGGCCTCAGTTGGAGCAACCGGGAGAAGGCAAAGCAGGCATTCAAGGAACTG CTGAGGGACAAGGCTGTCCCCTCCAATGCCTCATGGGAACAGGCCATGAAGATGGTGGTC ACCGACCCCCGTTACAGTGCCTTGCCTAAACTGAGTGAGAAAAAGCAGGCATTCAATGCC TACAAGGCGCAGCGGGAGAAGGAGGAGAAGGAGGAGGCCCGGCTAAGGGCCAAAGAGGCC AAGCAGACCCTGCAGCATTTCCTGGAGCAGCATGAACGCATGACCTCCACCACCCGCTAC CGGCGGGCAGAACAGACCTTTGGGGAGCTGGAGGTCTGGGCTGTGGTCCCTGAGAGGGAT CGAAAAGAGGTTTATGATGATGTCCTCTTCTTCCTGGCCAAGAAGGAGAAGGAACAGGCC AAGCAGCTCCGGCGCCGCAATATCCAGGCCCTAAAGAGCATCCTGGATGGGATGAGTAGT GTCAACTTCCAAACCACGTGGTCCCAGGCCCAGCAGTACCTCATGGATAACCCCAGCTTT GCTCAGGACCATCAGCTGCAGAACATGGACAAGGAAGATGCACTGATCTGTTTTGAGGAG CACATCCGAGCTTTGGAGAGGGAAGAGGAGGAGGAACGGGAGCGGGCCCGGCTTCGGGAG CGACGCCAACAACGCAAGAATCGGGAGGCCTTCCAGACCTTCCTGGACGAGCTGCATGAG ACAGGGCAGCTGCACTCTATGTCCACCTGGATGGAGCTATATCCAGCAGTCAGCACTGAT GTCCGCTTTGCCAACATGCTGGGCCAGCCGGGCTCCACCCCTCTGGACTTATTCAAGTTC TATGTGGAGGAGTTGAAGGCACGATTCCATGATGAAAAGAAGATCATTAAGGACATCCTT AAGGTGAACACGGCCTTTGAGGACTTCGCCCACGTCATAAGCTTTGACAAGAGGGCTGCC GCACTGGACGCAGGCAACATCAAGCTGACCTTCAATAGTCTGCTGGAGAAAGCAGAGGCA CGGGAGAGGGAGCGGGAGAAGGAGGAGGCACGCAGGATGCGGCGCAGGGAAGCTGCCTTT CGAAGCATGCTGAGGCAGGCTGTGCCTGCTCTGGAGCTAGGCACTGCCTGGGAAGAGGTC CGTGAGCGTTTTGTGTGTGACTCAGCCTTTGAGCAGATCACCCTGGAGTCGGAGCGGATC CGGCTCTTCCGGGAGTTCCTACAGGTGCTGGAGCAGACTGAATGCCAGCACCTCCACACC AAAGGCCGAAAGCATGGCAGGAAAGGCAAGAAGCACCATCACAAGCGTTCCCACTCACCT TCAGGCTCTGAGTCAGAAGAAGAGGAGCTGCCCCCACCATCTCTCCGGCCCCCCAAGCGG AGGAGGCGGAACCCCTCAGAGTCAGGCTCTGAGCCCTCTTCCTCACTTGATTCAGTTGAA AGTGGGGGTGCTGCCCTTGGAGGACGGGGCTCCCCTTCCTCCCATCTTCTTGGAGCAGAT CATGGCCTTCGGAAAGCCAAGAAACCAAAAAAGAAAACTAAGAAGAGAAGACACAAGTCG AATAGTCCTGAGAGTGAGACAGACCCTGAGGAGAAAGCTGGCAAGGAGAGCGATGAGAAA GAACAAGAACAGGACAAGGACAGGGAGCTCCAACAGGCAGAGCTCCCTAACCGTTCCCCA GGCTTTGGAATCAAGAAGGAGAAGACAGGCTGGGACACGTCAGAAAGTGAGCTGAGTGAG GGTGAGCTGGAGAGGCGGCGGCGGACACTCCTACAGCAGCTGGATGATCACCAGTGA
Restriction Sites	Please inquire
ACCN	NM_012272
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012272.1, NP_036404.1
RefSeq Size	3698 bp
RefSeq ORF	2577 bp
Locus ID	25766
UniProt ID	Q6NWY9
Protein Pathways	Spliceosome
Gene Summary	This gene encodes a WW-domain containing protein similar to yeast splicing factor PRP40. This protein has been shown to interact with Huntingtin and methyl CpG binding protein 2 (MeCP2). Alternative splicing results in different transcript variants. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1. It initiates translation at a downstream start codon. The encoded isoform (2) is shorter than isoform 1.

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